Regeneration of calabrian pine from juvenile needles

Adventitious shoot formation and plant regeneration from Pinus pinaster Sol. ex Aiton

“…After the induction phase, the transfer of the explants to DCR hormone-free medium containing AC was essential for shoot elongation. AC was previously reported as having a positive effect on elongation of axillary buds (7,12) and of adventitious buds on needles (1,9,10) of Pinus pinaster. We report a reduction in shoot elongation for the highest AC concentration, which is in agreement with the harmful effects of high AC concentration reported for Pinus contorta (33).…”

Micropropagation Via Axillary Bud Proliferation from Seedlings and Juvenile Shoots ofPinus patulaSchiedeetDeppe

“…Furthermore, according to Mott (1981) adequate shoot elongation is also very important for successful rooting. The elongation medium contained activated charcoal as this has been found to have a positive effect on shoot growth (Abdullah and Grace, 1987;George, 1996) and is a procedure adopted by most workers in the field (e.g. Jang and Tainter, 1991;Martinez Pulido et al, 1994).…”

Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability

“…The two outermost layers of cells correspond to palisade parenchyma, which is covered by an epidermis layer. Adventitious organs were induced only from the outermost parenchyma layers (Abdullah and Grace, 1987). Immature needles (Fig.…”