Micropropagation Via Axillary Bud Proliferation from Seedlings and Juvenile Shoots of<i>Pinus patula</i>Schiede<i>et</i>Deppe

Watt, M. P.; Ramgareeb, S.; Hope, B.; Blakeway, F. C.; Denison, N. P.

doi:10.1080/10295925.1998.9631173

Cited by 1 publication

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References 24 publications

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“…Within four and five weeks of culture, roots emerged at the base of some shoots while other shoots rooted after ten weeks of culture (Figure 3). This rooting rate in this period of time is relatively good when compared to other studies with Pinus that showed rooting varying between 40 and 50% in the same period (Cuesta et al, 2008;Stojičić et al, 1999;Watt et al, 1998;Gladfelter and Phillips, 1987;Scaltsoyiannes et al, 1994;Stiff et al, 1989). The mean number of roots per shoot was similar for all treatments, around 1.5 (Table 5).…”

Section: Root Medium Rooting (%)supporting

confidence: 65%

Propagation from axillary buds and anatomical study of adventitious roots of Pinus taeda L.

Le¹,

Oliveira²,

Quoirin³

et al. 2013

Afr. J. Biotechnol.

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Micropropagation of Pinus taeda by axillary bud proliferation technique and anatomical study of adventitious roots were studied. Apical and basal segments were established in vitro in Murashige and Skoog (MS) culture medium. For multiple shoot induction, the explants were transferred to WV3 medium with or without 6-benzylaminopurine (BAP) (0.12, 0.25 or 0.50 µM). The effect of the culture medium and combinations of α-naphthalene acetic acid (NAA) with BAP on adventitious rooting were evaluated. The basal segments exhibited higher multiplication rates (3.7 shoots per explant) than apical segments during establishment of in vitro culture. The best rooting rate (40%) was achieved by induction of roots in water-agar (WA) culture medium supplemented with 2.69 µM NAA and 0.44 µM BAP, for 9 days, followed by transfer into GDm/2 medium. Adventitious roots can be derived from the cambium cells, from adventitious meristem, or from a callus developed at the base of the shoots. When formed indirectly, roots can be derived from peripheral cells of the callus. In treatment resulting in the best rooting rate, 58.3% of the roots were formed directly. The callus formation at stem basis did not prevent rooting and did not impair acclimatization. The plantlets were successfully acclimatized with 90% survival.

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