Regeneration in vitro of the adult frog sciatic sensory axons

Edström, Anders; Edbladh, M.; Ekström, Per; Remgård, Pär

doi:10.3233/rnn-1990-13413

Cited by 10 publications

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“…Before discussing the effects of insulin, it seems appropriate to briefly deal with the question of initiation of mitosis, a matter of current debate. The present as well as earlier studies (Edbladh et al, 1990;Edstrom et al, 1990;Ekstrom, 1991) show that under culture conditions the frog sciatic nerve displays proliferation of Schwann cells, suggesting that the mitosis signal is to be found within the preparation. Likewise, proliferation of Schwann cells has been demonstrated in cat sciatic nerve explants (Crang and Blakemore, 1986).…”

Section: Discussionsupporting

confidence: 86%

“…Ara-C at a proliferation blocking concentration inhibited outgrowth when applied during the first 5 days of an 8 day incubation period. In contrast, it was without effect when applied during the last 3 days (Edbladh et al, 1990). In this preparation Schwann cell proliferation seems to influence the initiation of the axonal regrowth rather than its continuation.…”

Section: Introductionmentioning

confidence: 66%

“…Frogs ( R a m temporaria, The Frog Farm, Kelly, Ireland) were kept in water tanks at 20°C in an artificial 12 hr light/l2 hr dark cycle. Dissection of nerves were as described in detail previously (Edbladh et al, 1990). Briefly, the sciatic nerves were exposed, subjected to a crush lesion and then immediately dissected out with attached 8th and 9th dorsal root ganglia under sterile conditions.…”

Section: Materials and Methods Frog Nervesmentioning

confidence: 99%

“…However, the influence of proliferation on axonal regrowth could be restricted to the time immediately after the injury. Edbladh et al (1990) used a blocker of cell division, cytosine-P-D-arabinoside (ara-C) to study the effects of proliferation on axonal outgrowth in the in vitro regenerating frog sciatic nerve. Ara-C at a proliferation blocking concentration inhibited outgrowth when applied during the first 5 days of an 8 day incubation period.…”

Section: Introductionmentioning

confidence: 99%

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Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve

Ekström

Edbladh

Edström

1993

J of Neuroscience Research

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The present study showed that insulin (0.01 microgram/ml, approximately 2 nM) inhibited [3H]-thymidine incorporation in support cells, most likely Schwann cells, of the cultured frog sciatic nerve. A 25-35% inhibition took place in regenerating nerve preparations as well as in preparations devoid of neuronal protein synthesis, i.e., in isolated 5 mm nerve segments and in gangliectomized nerves, suggesting that the effect was direct and not mediated via the neuronal cells. The inhibition by insulin was time-dependent in that an effect was seen after 4 days but not at shorter or at longer periods of culturing. In separate experiments biotinylated insulin was shown to be taken up by Schwann cells in the regenerating nerve. Addition of serum increased the [3H]-thymidine incorporation severalfold and abolished the inhibitory action of insulin. Our results suggest that insulin, at a certain stage of the regeneration programme, exerts a direct, inhibitory effect on the proliferation of the Schwann cells in the cultured frog sciatic nerve.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 66%

Section: Materials and Methods Frog Nervesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve

Ekström

Edbladh

Edström

1993

J of Neuroscience Research

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“…In the present study I have sought to analyze the role of cAMP in nerve regeneration by using the in vitro regenerating adult frog sciatic nerve. This preparation displays both regrowth of sensory axons and injury-induced division of SC in a defined, serum-free culturing environment (Edstrom and Kanje, 1988;Edbladh et al, 1990;Edstrom et al, 1990). The advantages of these features are obvious.…”

Section: In Vivo Regenerationmentioning

confidence: 99%

Increased cyclic AMP in in vitro regenerating frog sciatic nerves inhibits Schwann cell proliferation bur has no effect on axonal outgrowth

Ekström¹

1995

J of Neuroscience Research

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In the present study the role of cAMP for axonal outgrowth and Schwann cell proliferation was studied using the cultured frog sciatic nerve. An intrinsic rise in nerve and ganglionic cAMP could be measured as a response to nerve injury, both in vitro and in vivo. Treatment with 0.1-1.0 microM forskolin, an activator of the cAMP-generating enzyme adenylyl cyclase, increased the cAMP content up to 13-fold, but was yet without effect on axonal outgrowth during an 8-day culturing period. HA-1004, an inhibitor of cAMP-dependent protein kinase, also lacked effect on the regeneration. In contrast, the proliferation of Schwann cells, measured as [3H]thymidine incorporation, was inhibited to about 70% of control by forskolin, whereas HA-1004 stimulated proliferation to approximately 130% of control. The results suggest that cAMP is involved in the injury-induced proliferation of Schwann cells of an adult peripheral nerve but that it lacks a central role in the regeneration of sensory axons of such nerves.

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Regeneration of an adult peripheral nerve preparation in culture

Kanje

1992

Mol Neurobiol

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The methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. A hypothesis is presented on the triggering of the cell body reaction. It is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. These proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process.

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Regeneration in vitro of the adult frog sciatic sensory axons

Cited by 10 publications

References 0 publications

Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve

Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve

Increased cyclic AMP in in vitro regenerating frog sciatic nerves inhibits Schwann cell proliferation bur has no effect on axonal outgrowth

Regeneration of an adult peripheral nerve preparation in culture

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