Regeneration from Leaf Explants of Bulgarian Raspberry Cultivars and Elites

Abstract

IntroductionThe development of regeneration procedures from various plant organs allows the establishment of useful genetic variation. Such procedures are an obligatory prerequisite of numerous biotechnology and classical breeding programs. This is particularly true for perennial crops such as the small fruits or fruit trees where the classical breeding process is rather extensive and prolonged.

Effects of calcium gluconate and ascorbic acid on controlling shoot necrosis during micropropagation of primocane-fruiting raspberry (Rubus idaeus L.) cultivars

“…Plant genotype often profoundly affects explant response to in vitro culture as proposed by Burbulis et al (2012). Similar observation on other raspberry cultivars was reported by Georgieva et al (2004) where raspberry cultivar 'Bulgarski Rubin' started shoot initiation three weeks after culture initiation while 'Shopska Alena' started one week later. The different responses of raspberry cultivars in in vitro culture might be caused by the endogenous hormonal balance in plant tissue as mentioned by Tanaka et al (2012) that the difference in adventitious bud regeneration between cultivars might be caused by differences in endogeneous hormones levels between cultivars.…”

Screening of Bulgarian Raspberry Cultivars and Elites for Osmotic Tolerance in Vitro

“…Elite 1 and Elite 3 were tested for osmotic stress tolerance. In vitro plants were obtained as previously described (7). In short: initial explants were taken form 1-year old shoots of 2-years old mother plants grown under greenhouse conditions.…”

Genetic Resources of small Fruits, Present and Future Development

“…The effective protocols for rooting and acclimatization of in vitro plants have also been described (18,25,30,42,46,50,72). The adaptation of protocols for micro propagation and rooting of raspberry depends in grate degree of genotypes.…”