Effects of calcium gluconate and ascorbic acid on controlling shoot necrosis during micropropagation of primocane-fruiting raspberry (Rubus idaeus L.) cultivars

Amalia, Fridia; Debnath, Samir C.; Yeoung, Young Rog

doi:10.5897/ajb2014.14201

Cited by 5 publications

(3 citation statements)

References 22 publications

(27 reference statements)

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“…Moreover, several species responded well to reduced MS salts. Amalia et al (2014) also noticed some (unquantified) reduction in STN of raspberry (Rubus idaeus L.) shoots when 50 or 100 mg/l (0.284-0.568 mM) of ascorbic acid was used, but not as effectively as the use of 1 g/l Ca-gluconate. The reduction in STN was also genotype dependent.…”

Section: Choice Of Basal Mediummentioning

confidence: 99%

“…Ca-gluconate has been reported as a way to alleviate STN in herbal medicinal plants (Srivastava and Joshi 2013), woody shrubs (Amalia et al 2014), fruit trees (Abousalim and Mantell 1994;Pérez-Tornero and Burgos 2000), and other trees ( De Block 1990;Pasqua et al 2002). As far as we know, the only report to assess the individual role of the gluconate − ion (C 6 H 11 O 7 − ) in plant growth and development was Nezami-Alanagh et al (2017).…”

Section: Interaction Of Other Ions On Stn: the Ion-confounding Effectmentioning

confidence: 99%

“…Increasing Ca 2+ in medium of several tree species has been shown to alleviate STN (McCown and Sellmer 1987). Cagluconate is an organic form of Ca that allows Ca 2+ to be released into aqueous solutions, explaining why it has occasionally been used to alleviate STN, but it negatively affects shoot growth (Singha et al 1990;Amalia et al 2014). We suspect that the use of gas-permeable culture vessels, such as the Vitron or Miracle Pack (Teixeira da Silva et al 2006), could reduce hyperhydricity, reduce the accumulation of ethylene, increase transpiration and consequently increase the transport of Ca 2+ to the shoot apical meristem, although this hypothesis has yet to be tested on in vitro plant cultures displaying STN.…”

Section: Calcium and Calcium Signalingmentioning

confidence: 99%

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Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

Silva

Nezami-Alanagh

Barreal

et al. 2020

Planta

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Main conclusion Shoot tip necrosis is a physiological condition that negatively impacts the growth and development of in vitro plant shoot cultures across a wide range of species. Abstract Shoot tip necrosis is a physiological condition and disorder that can arise in plantlets or shoots in vitro that results in death of the shoot tip. This condition, which can spread basipetally and affect the emergence of axillary shoots from buds lower down the stem, is due to the cessation of apical dominance. STN can occur at both shoot multiplication and rooting stages. One of the most common factors that cause STN is nutrient deficiency or imbalance. Moreover, the presence or absence of plant growth regulators (auxins or cytokinins) at specific developmental stages may impact STN. The cytokinin to auxin ratio within an in vitro plant can be modified by varying the concentration of cytokinins used in the culture medium. The supply of nutrients to in vitro shoots or plantlets might also affect their hormonal balance, thus modifying the occurrence of STN. High relative humidity within culture vessels and hyperhydricity are associated with STN. An adequate supply of calcium as the divalent cation (Ca2+) can hinder STN by inhibiting the accumulation of phenolic compounds and thus programmed cell death. Moreover, the level of Ca2+ affects auxin transport and ethylene production, and higher ethylene production, which can occur as a result of high relative humidity in or poor ventilation of the in vitro culture vessel, induces STN. High relative humidity can decrease the mobility of Ca2+ within a plant, resulting in Ca2+ deficiency and STN. STN of in vitro shoots or plantlets can be halted or reversed by altering the basal medium, mainly the concentration of Ca2+, adjusting the levels of auxins or cytokinins, or modifying culture conditions. This review examines the literature related to STN, seeks to discover the associated factors and relations between them, proposes practical solutions, and attempts to better understand the mechanism(s) underlying this condition in vitro.

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Section: Choice Of Basal Mediummentioning

confidence: 99%