Regenerated cellulose-silk fibroin blends fibers

Morphology and properties of cellulose/silk fibroin blend fiber prepared with 1-butyl-3-methylimidazolium chloride as solvent

“…The size distribution of soybean protein is about 50-100 nm. Marsano et al (2008) made cellulose/silk fibroin blend membranes and fibers with Nmethylmorpholine N-oxide hydrates (NMMO/H 2 O) as co-solvent. The phase size of silk fibroin is 2-10 lm in the blend membranes.…”

Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori

“…Silk fibroin was extracted from the degummed silk threads by using seven different extraction solutions and conditions with a ratio of 1:30 (w/v) of degummed silk to each extraction solution. These extraction solutions and conditions were Ajisawa's solution (calcium chloride:methanol:water in a mole ratio of 1:2:8) at 80 • C [12], 9 M lithium thiocyanate at 40 • C [13], 9 M lithium bromide at 60 • C [14], 65% lithium chloride at 40 • C, 75% lithium chloride at 40 • C and 85% lithium chloride at 40 • C and 60 • C. After completely dissolving silk threads, the fibroin extracted solution was filtered through a Whatman Paper No. 1, and dialyzed against distilled water for 24 h, 4 • C using cellulose tubular membranes (12,000-14,000 molecular weight cut-off; BDH, USA), followed by a lyophilized step to obtain dried silk fibroin powder.…”

“…To compare patterns of light-chain fibroin and heavy-chain fibroin obtained from different extraction means, fibroins of Thai silk cocoon (B. mori, local stain Nangnoisrisaket) were extracted by seven extraction methods at the ratio of 1:30 (w/v), including the extraction by Ajisawa's reagent at 80 • C [12], 9 M lithium thiocyanate (LiSCN) at 40 • C [13], 9 M lithium bromide (LiBr) at 60 • C [14], 65% lithium chloride (LiCl) at 40 • C and 75% LiCl at 40 • C, 85% LiCl at 40 • C [18] and 85% LiCl at 60 • C. The results are shown in Table 1, 9 M LiSCN could completely dissolve degummed silk within only 10 min, while Ajisawa's reagent used 15 min, and the other reagents took about 25-40 min. The extractions by 9 M LiBr and Ajisawa's reagents yielded high fibroin levels; 67.75% and 65.75% of cocoon weight, respectively.…”

“…In addition, different mass ratios between two fibroins should also affect the properties of produced scaffolds. To prepare silk fibroin for electrospinning, several extraction reagents were reported to extract silk fibroin from silk threads, including Ajisawa's reagent [12], 9 M lithium thiocyanate [13] and 9 M lithium bromide [14]. Although these fibroin extraction reagents are commonly used, there is no comparison on yield, protein integrity, type and mass ratio of light-chain fibroin and heavy-chain fibroin.…”

Effect of coagulation agents on morphology and structure of cellulose/silk fibroin films with 1-butyl-3-ethylimidazolium chloride as solvent

“…Nonetheless, when an ethanol-water mixture was used to coagulate blended cellulose/silk fibroin dissolved in N-methylmorpholine N-oxide (NMMO) a phase separation occurred. 6 Ethanol was also used to regenerate cellulose/silk fibroin from N, N-dimethylacetamide/LiCl (DMAc/LiCl). Even though there was no visible phase separation, microvoids and a low degree of crystallinity in the blend structures was reported.…”