Regeneração de raízes e plântulas de sementes de Eugenia spp. (Myrtaceae) de diferentes estádios de maturação

Delgado, Liliana Ferreira; Barbedo, Claudio José

doi:10.1590/2236-8906-04/2020

Cited by 6 publications

(2 citation statements)

References 25 publications

(37 reference statements)

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“…Some species of this genus, for example, have shown considerable capacity in tolerating water deficit conditions for the germination and the initial phases of seedling establishment (McLaren and McDonald, 2003;Inocente and Barbedo, 2019), as well as for tolerating flooded conditions (Calvi et al, 2017b). In addition, these seeds can germinate and produce new plants even when a large part of their reserve tissue is lost, for example, through predation (Teixeira and Barbedo, 2012;Alonso et al, 2019;Alonso and Barbedo, 2020;Delgado and Barbedo, 2020).…”

Section: Resultsmentioning

confidence: 99%

Regeneration of roots and seedlings from Eugenia involucrata seeds under water deficit conditions

Inocente

Barbedo

2021

J. Seed Sci.

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The aim of this study was to analyze the regenerative capacity of Eugenia involucrata seeds as a possible strategy to ensure the production of new seedlings when they are going through a period of water deficit. Progressive water deficit conditions over time (up to 60 days for seeds and 270 days for seedlings) and in intensity (up to -5 MPa for seeds and -10 MPa for seedlings) were simulated, and seedling regeneration was analyzed. The results showed that these seeds can survive even under the most extreme conditions (-5 MPa for 60 days) and some seedling tissues as well (-5 MPa for 270 days). Furthermore, roots and seedlings continued to develop from apparently necrotic tissue on the seed surface, showing that, when necessary, these seeds use their mechanism of formation of new roots and seedlings to ensure species survival under water stress conditions.

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Section: Resultsmentioning

confidence: 99%

Regeneration of roots and seedlings from Eugenia involucrata seeds under water deficit conditions

Inocente

Barbedo

2021

J. Seed Sci.

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“…Entretanto, a formação da parte aérea foi afetada negativamente em sementes fracionadas na metade, apresentando apenas 54% de plântulas completas, 20% em ganho de produção de plântulas quando comparado ao tratamento de sementes intactas, devido à redução de reserva das sementes (GOMES et al, 2016). A formação de plântulas completas em sementes fracionadas decai, não sendo garantida pela presença do processo germinativo a partir de frações, por apresentar menor quantidade de reservas disponíveis no seu desenvolvimento (SILVA et al, 2003;TEIXEIRA;BARBEDO, 2012;ALONSO et al, 2019;BARBEDO, 2020).…”

Section: Sacarose (G Lunclassified

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

Júnior¹,

de²

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In vitro cultivation of cerejeira-do-Rio-Grande (Eugenia involucrata DC.) and cambucizeiro (Campomanesia phaea (O. Berg) Landrum) Brazilian biodiversity is one of the richest in the world with different biomes that comprise its flora and fruit species with great commercial exploitation value. The Atlantic Forest, the third-largest biome in the country, is considered a global hotspot for conservation and biological diversity, with numerous native fruits, including species of the Myrtaceae family, such as the cerejeira-do-Rio-Grande (Eugenia involucrata DC.) and the cambucizeiro (Campomanesia phaea (O. Berg) Landrum). These species have great potential for commercial use, in addition to being extremely important for reforestation and urban afforestation. The cerejeira-do-Rio-Grande and cambucizeiro fruits have high nutritional value, being commercialized fresh or processed, contributing to the diversification of production of Brazilian fruit growers. The commercial multiplication of these fruit trees is carried out through the seminal route and is hampered due to the low storage potential of the seeds. Conventional methods of asexual multiplication in both fruit trees are inefficient, and scientific studies willing to optimize them are scarce. Micropropagation, multiplication of plants through in vitro culture techniques, is an alternative method for large-scale production of commercial seedlings of these fruit trees. Therefore, this work aimed to establish protocols of germination and in vitro multiplication of cerejeira-do-Rio-Grande using nodal explants from seedlings germinated in aseptic conditions and to evaluate the different processes and culture medium components for the establishment and multiplication of nodal and leaf explants of cerejeira-do-Rio-Grande and cambucizeiro, originated from plants grown in the greenhouse. The best conditions for seed germination and seedling development of cerejeira-do-Rio-Grande are when they are cultivated in an MS medium supplemented with 15 g L -1 of sucrose, removing the tegument and cultivating in the dark; for in shoot induction and development, the use of 2.5 mg L -1 of 6benzylaminopurine (BAP) and JADS culture medium is recommended; in the subculture, the best results were obtained when adding 1.0 to 2.5 mg L -1 of BAP; finally, it is possible to obtain high rates of rooting of shoots when using WPM, ½ WPM, ¼ WPM, and JADS culture media without any growth regulators. The seedling acclimatization is achieved by using a commercial potting mix (based on pine bark, peat, and vermiculite) mixed with vermiculite in a 1:1 ratio. For the in vitro culture of cerejeira-do-Rio-Grande, explants from plants kept in the greenhouse, the lowest contamination rate, oxidation, and greater survival rate of nodal explants, the collection must be carried out in the autumn, performing the asepsis with sodium hypochlorite (NaClO) containing 1.25% of active chloride. The addition of 1.0 mg L -1 of BAP in the presence of 0.1 mg L -1 of ANA (naphthalene acetic acid) and 2.5 mg L -1 of BAP e...

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The origin of new roots from cut seeds of Eugenia species

et al. 2022

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Studies on seed germination in Eugenia species after embryo fractionation have demonstrated that cotyledon cells have capacity for de-differentiation and consequent production of roots. However, there is no information about the origin of those new roots. Thus, the aim of this study was to characterize anatomically the cotyledon regions of seeds of five species of Eugenia to elucidate the tissue that originates such roots. Seeds were sectioned across the hillum region and immediately placed to germinate. As soon as the fractions of these cut seeds develop roots they were fixed and processed to the usual techniques for light microscopy. The adventitious roots originated only when the seed was fractioned, never occurring spontaneously in uninjured seeds. Adventitious roots were formed from perivascular parenchyma cells, located close to the injured region of the cotyledons. These cells divided periclinally and proliferated, giving rise to a root meristem. Therefore, new seedlings probably have a different genome than the mother plant, but they would be identical to the embryo that was a result of fertilization.

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Regeneração de raízes e plântulas de sementes de Eugenia spp. (Myrtaceae) de diferentes estádios de maturação

Cited by 6 publications

References 25 publications

Regeneration of roots and seedlings from Eugenia involucrata seeds under water deficit conditions

Regeneration of roots and seedlings from Eugenia involucrata seeds under water deficit conditions

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

The origin of new roots from cut seeds of Eugenia species

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