Regenerable fiber-optic-based immunosensor

Abstract

An immunosensor is described that is based on fluorescently labeled F(ab') anti-human serum albumin antibody fragments covalently immobilized to the distal end of a fiber-optic probe. When human serum albumin is present, it is bound to the sensor and shields the fluorescent label from the solvent water, and a significant increase in the label fluorescence results. The sensor can be regenerated by simply immersing the sensing tip in chaotropic media. Under these conditions the antigen-antibody complex is selectively disrupted without adversely affecting the sensor. In the present configuration, the same sensor can be recycled over 50 times before the immunosurface inactivates significantly. With proper storage the sensor can last for up to 4 months.

Adsorption Dynamics of IgG and Its F(ab′)2and Fc Fragments Studied by Reflectometry

“…They were kindly donated by tests have been developed by immobilizing only the F(ab) Organon Teknika (Boxtel, The Netherlands). Both IgG's fragments (7,8) or F(ab) 2 fragments (9).…”

On‐Off PVC Membrane Based Potentiometric Immunosensor for Label‐Free Detection of Alpha‐Fetoprotein

“…Regeneration of the immunosensor can significantly reduce cost of analysis compared to disposable immunosensors. However, the immobilized immunoreagent or immunocomplex could also suffer from the functional damage of immunoactivity because the regeneration reagent provide drastic conditions (e.g., in alkaline or acidic solutions or with chaotropic agents) [18,19]. In this experiment, the disposable immunosensor was employed because of its low cost and easy fabrication.…”

Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti‐PCB Antibody

“…Some immunosensors require replacement of the chemistry at the device surface, which is costly and time consuming [17][18][19]. Efforts to prepare analytical devices with regenerable active antibody sites have made use of acidic [14,15] or chaotropic media [16]; this however might permanently alter the antibody conformation and result in loss of activity. A few reports have described the regeneration of active sites of antibodies by simply washing with a flowing electrolyte solution (i.e., mass action) [22][23][24].…”

“…An immunosensor based on the BLM transduction scheme should be regenerable and capable of multiple analyses. A number of immunosensors have been regenerated by placing the device in acidic [14,15] or in chaotropic media [16]. Regeneration of BLMs that contain antibody by using acidic conditions can not be done as this can disrupt membrane structure and can induce the formation of ion channels [39].…”