On‐Off PVC Membrane Based Potentiometric Immunosensor for Label‐Free Detection of Alpha‐Fetoprotein

Zhou, Lu; Yuan, Ruo

doi:10.1002/elan.200603836

Cited by 13 publications

(6 citation statements)

References 21 publications

(14 reference statements)

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“…Reusing of an immunosensor is very appealing due the possibility of application to many samples in a short period at a low cost relatively to the disposable ones [54]. Accordingly, several regeneration reagents which have already been successfully used by Park et al [55].in 2000 were tested.…”

Section: Regeneration and Stability Of The Immunosensormentioning

confidence: 99%

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Silva

Magalhães

Barroso

et al. 2019

Talanta

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Polymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization. Additionally, a marker ion was used to detect the antibody-antigen binding event at the electrode surface. The immunosensor construction was performed in several steps: i) gold salt ions extraction in PVC membrane; ii) AuNPs formation using Na 2 EDTA as reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in pipette-tip electrodes. The potential shift observed in potentiometric measurements was derived simply from the blocking effect in the ionic flux caused by antigen-antibody conjugation, without no extra steps, mimetizing the ion-channel sensors. A detection limit of 6 cells mL −1 was attained. As proof-of-concept, recovery studies were performed in spiked commercial apple juice samples with success. Due to the simplicity of use, the appealing cost of equipment and sensor production and being able to provide a quick analytical response (less than 1 h for a complete assay, including sample preparation for analysis), this scheme represents a good prototype device for the detection of foodborne pathogens like ST or other immune-responsive bacteria.

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Section: Regeneration and Stability Of The Immunosensormentioning

confidence: 99%

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Silva

Magalhães

Barroso

et al. 2019

Talanta

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show abstract

“…The pH value of the detection solution was also an important parameter, and unsuitable pH may cause protein denaturalization or immunosensor unstable. 38 As shown in Fig. 5B, the peak current change was increased with the increment of pH value from 5 to 7 and then decreased.…”

Section: Optimization Of Determination Conditions For Immunoassaymentioning

confidence: 71%

Electrochemical Immunosensor for Aflatoxin B<sub>1</sub> Based on Polyaniline/Graphene Nanohybrids Decorated with Au Nanoparticle

et al. 2017

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A novel method for preparing polyaniline/graphene (PANI/GN) nanocomposites was demonstrated by liquid-liquid interface polymerization method. Then the gold nanoparticles (Au NPs) were uniformly decorated on the PANI/GN surface to form gold/polyaniline/graphene (Au/PANI/GN) nanohybrids. The Au/PANI/GN nanohybrids film showed good electron transfer ability, which ensured high sensitivity to detect AFB 1 in a range from 0.05 to 25 ng/mL with a detection limit of 0.034 ng/mL obtained at 3σ (n = 10). The proposed immunosensor provided a simple tool for AFB 1 detection.

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“…The membrane composition was as follows: 3.0 wt% o-phenylenediamine, 29.8 wt% membrane solvent DOP and 67.2 wt% PVC. The o-phenylenediaminemodified PVC membrane was fixed on the easy-replace plastic part and joint with PVC tube [27]. After washing with distilled water, the aminated PVC membrane electrode was dipped into 16-nm gold colloids for 4 h at 4 8C.…”

Section: Preparation Of Multifunctional Pvc Membrane Electrodementioning

confidence: 99%

Double‐Layer Nanogold and Poly(amidoamine) Dendrimer‐ Functionalized PVC Membrane Electrode for Enhanced Electrochemical Immunoassay of Total Prostate Specific Antigen

Wang¹,

Tao²,

Meng³

2009

Electroanalysis

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A simple and portable electrochemical immunosensor for the detection of total prostate specific antigen (t-PSA) in human serum was developed using a double-layer nanogold particles and dendrimer-functionalized polyvinyl chloride (PVC) membrane as immunosensing interface. To fabricate such a multifunctional PVC electrode, an o-phenylenediamine-doped PVC membrane was initially constructed, then nanogold particles and poly(amidoamine) G4-dendrimer with a sandwich-type format were assembled onto the PVC membrane surface, and then t-PSA antibodies (anti-PSA) were adsorbed on the nanogold surface. The detection principle of the immunosensor is based on the change in the electric potential before and after the antigen-antibody interaction. The experimental conditions and the factors influencing the performance of the immunosensor were investigated. Under optimal conditions, the proposed immunosensor exhibits good electrochemical behavior in the dynamic range of 0.5 -18 ng/mL relative to t-PSA concentration with a relative low detection limit of 0.1 ng/mL (S/N ¼ 3). The precision, reproducibility, and stability of the immunosensor are acceptable. In addition, 43 serum specimens were assayed by the as-prepared immunosensor, and consistent results were obtained in comparison with those obtained by the standard enzymelinked immunosorbent assay (ELISA). Compared with the conventional ELISAs, the developed immunoassay system was simple and rapid without labeling and separation steps. Importantly, the immobilization and detection methodologies could be extended for the immobilization and detection of other biomarkers.

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On‐Off PVC Membrane Based Potentiometric Immunosensor for Label‐Free Detection of Alpha‐Fetoprotein

Cited by 13 publications

References 21 publications

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Electrochemical Immunosensor for Aflatoxin B<sub>1</sub> Based on Polyaniline/Graphene Nanohybrids Decorated with Au Nanoparticle

Double‐Layer Nanogold and Poly(amidoamine) Dendrimer‐ Functionalized PVC Membrane Electrode for Enhanced Electrochemical Immunoassay of Total Prostate Specific Antigen

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