Refractive Index-Based Detection of Gradient Elution Liquid Chromatography using Chip-Integrated Microring Resonator Arrays

Wade, James H.; Bailey, Ryan C.

doi:10.1021/ac4035828

Cited by 35 publications

(48 citation statements)

References 30 publications

(53 reference statements)

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“…Unlike differential refractometers, microring resonators arrays are compatible with both isocratic and gradient separations methods. Proof-of-concept experiments have been performed on small molecule therapeutics (e.g., ibuprofen) (131). Optical resonators can also been integrated with other analytical tools, such as scanning probe microscopy.…”

Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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Optical resonator sensors are an emerging class of analytical technologies that use recirculating light confined within a microcavity to sensitively measure the surrounding environment. Bolstered by advances in microfabrication, these devices can be configured for a wide variety of chemical or biomolecular sensing applications. The review begins with a brief description of optical resonator sensor operation followed by discussions regarding sensor design, including different geometries, choices of material systems, methods of sensor interrogation, and new approaches to sensor operation. Throughout, key recent developments are highlighted, including advancements in biosensing and other applications of optical sensors. Alternative sensing mechanisms and hybrid sensing devices are then discussed in terms of their potential for more sensitive and rapid analyses. Brief concluding statements offer our perspective on the future of optical microcavity sensors and their promise as versatile detection elements within analytical chemistry.

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Section: Applications Of Optical Resonatorsmentioning

confidence: 99%

Applications of Optical Microcavity Resonators in Analytical Chemistry

Wade

Bailey

2016

Annual Rev. Anal. Chem.

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“…Another group of refractive index-based sensors have attractive specifications as non-destructive and universal detectors for liquid chromatographic separations, but a short dynamic range and sensitivity to minor thermal perturbations limit the utility of commercial refractive index detectors for many possible usages, especially those needs the use of gradient elution [22].…”

Section: Introductionmentioning

confidence: 99%

Refractometric study on binary, ternary, and quaternary solutions made by water, methanol, ethanol, glycerol, D-glucose monohydrate (DGMH), sucrose, and sodium chloride at T = 293.15 K and atmospheric pressure

Koohyar

Nasiri²,

Kiani

2019

J. Mex. Chem. Soc.

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The glycerol, D-glucose monohydrate (DGMH), sucrose, and sodium chloride are used in food industries and the measurement of properties for these components and their aqueous solutions can be important. In this research work, the refractive indices for binary solutions of (methanol + glycerol), (ethanol + glycerol), ternary solutions of (water + glycerol + DGMH), (water + glycerol + sucrose), (water + sucrose + DGMH), (water + sucrose + ethanol), (water + ethanol + DGMH), (water + NaCl + DGMH), (water + methanol + NaCl), (water + ethanol + NaCl), (water + NaCl + glycerol), (water + sucrose + NaCl), and quaternary solutions of (water + ethanol + sucrose + DGMH), (water + ethanol + sucrose + glycerol), (water + NaCl + sucrose + glycerol) were measured in wide range of mole fractions at T = 293.15 K and atmospheric pressure. For binary solutions of this study, the changes of refractive index on mixing, ∆nD, were calculated in each mole fraction at T = 293.15 K. Also, the refractive index of binary solutions was fitted by a semi-empirical equation. The constant of this equation, Kr, was represented by Koohyar et al. in 2011. This constant can be used to investigate power of interactions between solute and solvent molecules. For ternary and quaternary solutions of this study, a semi-empirical equation was used to determine refractive indices at given temperature. The comparison between calculated and experimental refractive indices shows that there is a good agreement between them especially in lower molal concentrations.

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“…Additionally, RID is sensitive to temperature fluctuation and has relatively poor sensitivity in the milligram per liter range. 33 These impediments eliminate the possibility of using gradient elution as the reference cell requires time for optical and thermal equilibration. 33,34 There are several factors of selectivity gained by hyphenating LC with MS, such as the formation of ions and potential fragmentation.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Methods for Quantitation of Sugars during the Corn-to-Ethanol Fermentation Process

et al. 2020

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The quantitation of sugars, including glucose, the primary fermentable sugar; maltose (DP2); and maltotriose (DP3), is a standard procedure during the corn-to-ethanol fermentation process. The quantitation of glucose by the Megazyme Assay utilizing glucose oxidase and peroxidase enzymes (GOPOD) and UV-Vis detection, high-performance liquid chromatography with refractive index detection (HPLC-RID), and liquid chromatography mass spectrometry (LC-MS) with electrospray ionization (ESI) and selected ion monitoring (SIM) was studied. Three biological flask fermentation replicates were analyzed every 12 h beginning at 14 h of fermentation (T14) until near completion of fermentation (T62). The method comparison results for glucose quantitation showed that the LC-MS SIM analysis had the lowest limit of quantitation (LOQ) at 2 ppm and the widest dynamic range of 2.7 orders of magnitude. The HPLC-RID analysis had a linear dynamic range (LDR) of 1.5 orders of magnitude with an LOQ of 1500 ppm. The Megazyme GOPOD analysis had an LDR of 0.9 orders of magnitude with an LOQ of 120 ppm. The HPLC-RID method was ideal for glucose quantitation when it was present in high concentrations. In contrast, maltose and maltotriose components were found to be present in lower concentrations, such that simultaneous quantitation of the three analytes is difficult during fermentation. The LC-MS method was the only method able to quantify the concentration of glucose successfully and simultaneously with DP2 and DP3 in all the fermentation broth samples collected from T14 through T62 during the corn-to-ethanol fermentation process.

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Refractive Index-Based Detection of Gradient Elution Liquid Chromatography using Chip-Integrated Microring Resonator Arrays

Cited by 35 publications

References 30 publications

Applications of Optical Microcavity Resonators in Analytical Chemistry

Applications of Optical Microcavity Resonators in Analytical Chemistry

Refractometric study on binary, ternary, and quaternary solutions made by water, methanol, ethanol, glycerol, D-glucose monohydrate (DGMH), sucrose, and sodium chloride at T = 293.15 K and atmospheric pressure

A Comparative Analysis of Methods for Quantitation of Sugars during the Corn-to-Ethanol Fermentation Process

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