Refolding and Activation of Human Prorenin Expressed inEscherichia coli: Application of Recombinant Human Renin for Inhibitor Screening

Reduction of Blood Pressure by Soybean Saponins, Renin Inhibitors from Soybean, in Spontaneously Hypertensive Rats

“…Aliskiren, a novel orally effective direct renin inhibitor, has recently been approved for treating hypertension in the United States, European Union, and Japan. 2) We have already screened the human renin inhibitor from wild plants and foods, 3) and isolated soyasaponin I (also known as soyasaponin Bb), 4) a triterpenoid type of saponin, from soybean (Glycine max) as an active compound. 5) We investigated in this present study whether soybean saponin containing soyasaponin I could inhibit human renin activity in vitro, and have an anti-hypertensive effect on spontaneously hypertensive rats (SHR).…”

Inhibition of human renin activity by saponins

“…Recently, we developed a method of rapid purification of recombinant human (rh) renin expressed in a baculovirus-insect cell system (1,2,9). Using rh-renin as a target enzyme we screened various foodstuffs and found that fermented soybean paste (miso) exhibits renin inhibitory activity originating from soybean (15). Moreover, minor legumes (legumes except soybean and peanuts) also exhibit renin inhibitory activity and can be divided into two groups: high-renin-inhibitory and low-renin-inhibitory groups (10).…”

The Occurrence of Renin Inhibitor in Rice: Isolation, Identification, and Structure-Function Relationship

“…[2][3][4][5] Although renin is the most important enzyme in the RAS, screening for renin inhibitors among foodstuffs has not been well studied due to the complications of the renin assay. Recently, we screened for renin inhibitory compounds in various foodstuffs using recombinant human (rh)-renin as target enzyme, [6][7][8] and found that fermented soybean paste miso contains a renin inhibitory compound, soyasaponin I, which originates in soybean. 9) In this study, we screened for renin inhibitory activity in various foodstuffs using rh-renin and renin-specific substrate, and found renin inhibitory activity in rice.…”