The Occurrence of Renin Inhibitor in Rice: Isolation, Identification, and Structure-Function Relationship

Inhibitory effect of catechin-related compounds on renin activity

“…It was reported that soybean saponin could inhibit human renin with an IC 50 value of 59.9 μg/mL (8). Fatty acids recognized as renin inhibitors in rice had an IC 50 value range of 28.3-41.1 μM (22). It was also confirmed that baicalin exerted renin inhibition with an IC 50 value of 120.36 μM (6).…”

“…It was suggested that EGCg exerted the bioactivity by forming an enzyme-substrate-inhibitor complex, which might influence the conformation of the active site of renin and consequently result in the loss of its enzymatic activity. The limited studies that have been performed in this regard reported that an uncompetitive inhibition pattern against renin was observed for the polyphenolic extract of Gongronema latifolium leaves (2) and flaxseed protein hydrolysate (23), while oleic acid and linoleic acid exhibited their renin-inhibitory activities as competitive inhibitors (22). It was found that gallated catechins, that is, ECg and EGCg, showed higher renin-inhibitory activities than the ungallated forms, suggesting that esterification with gallic acid could lead to quite favorable influence on elevating the renin-inhibitory activities of EC and EGC.…”

“…It has been reported that, for the renin-inhibitory activity of saponins, it was essential to have a glucuronic acid residue present at the first inner position of the 3β-hydroxyl sugar chain (21). In another study, the structure-function relationships of fatty acids were investigated, and it was discovered that unsaturated fatty acids exhibited inhibitory activities on recombinant human renin, while such an effect was not detected in saturated fatty acids (22). All these results implied that the renin-inhibitory activities from different categories of compounds could be attributed to the presence of special functional structures.…”

Reduction of Blood Pressure by Soybean Saponins, Renin Inhibitors from Soybean, in Spontaneously Hypertensive Rats

“…The same method as that for renin inhibition was used to measure the IC 50 value for pepstatin, a specific inhibitor of aspartic protease, as 20.4 mM, and it was reported that oleic acid and linoleic acid competitively inhibited renin activity, with IC 50 ¼ 28:3 and 37.4 mM, respectively. 8) In addition, we have previously reported that an IC 50 value of soyasaponin I for human renin of 30 mg/ml (31.8 mM), 4) while Kitagawa et al have reported that the content of soyasaponin I in total soyasaponin of soybean was about 50%. 9) Given that other soyasaponins (e.g., soyasaponin II (also known as soyasaponin Bc)) also inhibited human renin, 10) the IC 50 value for soybean saponin for human renin appears to be reasonable.…”

Chitinase fromAutographa californicaMultiple Nucleopolyhedrovirus: Rapid Purification from Sf-9 Medium and Mode of Action

“…1) Using this system, we easily produced and purified rh-renin, which was then used to survey human renin inhibitors. [2][3][4] During the expression and purification of rh-renin by baculovirusinfected Sf-9 cells, we found a large quantity of chitinase that was expressed in the culture medium at the late stage of cultivation. The chitinase was also adsorbed onto a pepstatin-affinity column, which is used for renin purification.…”