Reflexo do desequilíbrio ambiental na saúde: bactérias multirresistentes em ambiente hospitalar

Silva, Hyan Ribeiro da; Rocha, Álvaro Sepúlveda Carvalho; Rocha, Marcos Vitor Silva; Veras, Danielly Matos; Sousa, Olga Maria Castro de; Sousa, Gabriela da Costa; Almeida, Daniella Farias; Oliveira, Ana Lívia Paulino de; Bezerra, Gabrielly Soares Silva; Ribeiro, Dayse Anne dos Santos; Pereira, Nadia Maia; Alves, Ana Klara Rodrigues; Alves, Ana Karla Rodrigues; Nogueira, Fábio Dias; Rodrigues, Sandra Luiza Gouvêa; Pessoa, Gerson Tavares

doi:10.33448/rsd-v9i8.5604

Cited by 2 publications

(2 citation statements)

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“…Thus, the mechanism of action of this complex is similar to heparin, cyclodextrins derivatives (Karginov et al, 2007;Teixeira et al, 2009;Melo et al, 2015) and microcystins (Júnior et al, 2019). Those finding show tamoxifen as a putative drug to control multi-resistant bacteria in hospital environment (Silva et al, 2020).…”

Section: Resultsmentioning

confidence: 81%

Tamoxifen inhibits the anion channel induced by Staphylococcus aureus α-hemolysin: electrophysiological and docking analysis

Teixeira¹,

Júnior

Vieira³

et al. 2021

RSD

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To investigate the effects of tamoxifen on Staphylococcus aureus α-hemolysin channel (α-HL) in planar lipid bilayers with electrophysiological characterization and docking studies. Planar lipid bilayer membranes were prepared and α-HL (0.07 mg/mL) was added to the standard solution in cis compartment of the experimental chamber. All experiments were performed at room temperature using an Axopatch 200A amplifier in the voltage clamp mode. At pH 7.5, α-HL channels were usually in a high conductance ~4 nS and rarely switch to low conductance states. After the ion channel was incorporated in bilayer membrane, the tamoxifen was also added to the standard solution to the cis compartment. To docking studies, atomics coordinates for the α-HL heptameric channel was retrieved from PDB ID (7AHL) and the structure of tamoxifen was removed from the Pubchem, their coordinates were built and minimized with Avogadro software. The molecular docking experiments were performed using the Dockthor online portal. The tamoxifen inhibited (P < 0.05) α-HL channel conductance and it was a voltage-dependent manner. The three best docking solutions and the α-HL channel were evaluated, it was observed the connection mode with the highest affinity of interaction has a greater number of types of polar interaction. The residues present interactions of greater energy were 111 and 147 that form the remainders of the constriction in the channel of α-HL. The other conformers were accommodated in a region with more hydrophobic characteristics (valine 149). The mechanism of Staphylococcus aureus α-hemolysin inhibition by tamoxifen was blockade over the constriction of channel.

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Section: Resultsmentioning

confidence: 81%

Tamoxifen inhibits the anion channel induced by Staphylococcus aureus α-hemolysin: electrophysiological and docking analysis

Teixeira¹,

Júnior

Vieira³

et al. 2021

RSD

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“…Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), which is one of the main agents of nosocomial life-threatening infections, due to its difficult treatment (Silva et al, 2020), Yersinia enterocolitica, Salmonella enterica and Campylobacter spp. are pathogens of great one health concern and have already been isolated from the gastrointestinal tract of wild animals (Centers for Desease Control and Prevention -CDC, 2017;Chambers, 2001;Porrero et al, 2013;Silveira et al, 2018;Gomes et al, 2011;Dias, 2015).…”

Section: Introductionmentioning

confidence: 99%

MRSA and enterobacteria of one health concern in wild animals undergoing rehabilitation

Silveira

Moraes

Kaefer

et al. 2021

RSD

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The presence of contaminated animals in wildlife rehabilitation centers poses a threat for both animals and humans that come into contact with them or the contaminated environment. The aim of this study was to assess the presence of Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), as well as studying the biofilm formation capacity of these isolates, Yersinia enterocolitica, Salmonella enterica and Campylobacter spp. in wild animals from a rehabilitation center. During a two-year period, feces were collected from animals that were admitted to a wildlife rehabilitation center (n=324 samples). The pathogens were isolated from 51 (15.7%) animals of different species of mammals, birds and reptiles. Forty isolates (12.3%) were identified as S. aureus, of these, 32 (9.9%) were identified as MRSA and 72.5% were able to form biofilm. Y. enterocolitica was found in five mammals (5.1%), three reptiles (21.43%) and two birds (0.94%). Salmonella and Campylobacter were isolated from one bird each (0.67% and 0.67%, respectively). A wide diversity of animal species in rehabilitation centers, including birds, mammals and reptiles, can carry MRSA and enterobacteria of one health concern and eliminate in the feces. The presence of these pathogens in the gastrointestinal tract of wild animals admitted to a wildlife rehabilitation center shows the importance of microbiological monitoring of animals at the time of their admission and reinforces the need for specific hygienic-sanitary care.

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Reflexo do desequilíbrio ambiental na saúde: bactérias multirresistentes em ambiente hospitalar

Cited by 2 publications

References 0 publications

Tamoxifen inhibits the anion channel induced by Staphylococcus aureus α-hemolysin: electrophysiological and docking analysis

Tamoxifen inhibits the anion channel induced by Staphylococcus aureus α-hemolysin: electrophysiological and docking analysis

MRSA and enterobacteria of one health concern in wild animals undergoing rehabilitation

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