Refined Localization of Human Peroxisomal 3-Oxoacyl-CoA Thiolase (ACAA) to 3p22

Ottone, Francesca; Raimondi, Elena; Rocchi, Mariano; Giussani, F.; Malcovati, Massimo; Tenchini, Maria Luisa

doi:10.1159/000154263

Cited by 2 publications

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“…The genes of most of the peroxisomal β-oxidation enzymes have been assigned recently to different human chromosomes, either by chromosomal in situ hybridization or by molecular biological analysis of rodent/human somatic cell hybrid panels, revealing no arrangement in gene clusters (Bout et al 1989;Hoefler et al 1994;Varanasi et al 1994;Ottone et al 1995;Baumgart et al 1996b;Moghrabi et al 1997). Despite strong conservation of partial sequences in both human oxidase mRNAs, no cross-hybridization between the palmitoyl-CoA oxidase gene or branched-chain acyl-CoA oxidase gene was observed .…”

Section: Figmentioning

confidence: 99%

Application of in situ hybridization, cytochemical and immunocytochemical techniques for the investigation of peroxisomes

Baumgart

1997

Histochemistry and Cell Biology

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In situ hybridization, cytochemical and immunocytochemical techniques have contributed significantly to the understanding of the biology of peroxisomes, since they permit in situ demonstration of the sites of synthesis and distribution of peroxisomal proteins without the necessity of homogenization and subcellular fractionation of tissues or cultured cells. This article reviews the results of research on mammalian peroxisomal metabolism, biogenesis and proliferation in which morphological techniques have played a significant role in the elucidation of the biological problem. Some new data on peroxisomal heterogeneity and morphogenesis are included. The morphological methods applied have made it possible to characterize the differences in distribution of mRNAs encoding peroxisomal proteins in different tissues, as well as to monitor the marked heterogeneity in the protein composition and in the activity of specific enzymes in the peroxisomal population of single cells, or in tissues with complex organization (e.g. liver and kidney). In addition, the dynamic alterations and high plasticity of the peroxisomal compartment--partly dependent on contact of the peroxisomes to the microtubular network-are presented.

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