Application of in situ hybridization, cytochemical and immunocytochemical techniques for the investigation of peroxisomes

Baumgart, Eveline

doi:10.1007/s004180050160

Cited by 32 publications

(30 citation statements)

References 126 publications

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“…The kidney tissues at postnatal Day 28 ( Figure 2H) and Week 10 ( Figure 2I), whose cortices were positively stained in the full thickness, exhibited a characteristic staining pattern, with the straight part or S3 segment of the proximal tubules in the outer stripe of the outer medulla and medullary rays being most intense. This staining pattern for catalase has been reported in mature kidney tissues in previous studies employing the DAB reaction and in situ hybridization (Beard and Novikoff 1969;Schad et al 1996;Baumgart 1997). The absorption of the antibody with the antigen made the intensity of staining apparently decrease ( Figure 2J).…”

Section: Light Microscopic Immunohistochemistrysupporting

confidence: 84%

Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues

Johkura

Usuda

Liang

et al. 1998

J Histochem Cytochem.

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SUMMARYWe studied the developmental changes in the localization of peroxisome-specific enzymes in rat kidney tissues from embryonic Day 16 to postnatal Week 10 by immunoblot analysis and immunohistochemistry, using antibodies for the peroxisomal enzymes catalase, D-amino acid oxidase, L-␣-hydroxyacid oxidase (isozyme B), and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein. Peroxisomal enzymes were detected in the neonatal kidney by immunoblot analysis and their amount increased with kidney development. By light microscopic immunohistochemistry, they were first localized in a few proximal tubules in the juxtamedullary cortex of 18-day embryos. The distribution of proximal tubules positive for them expanded towards the superficial cortex with development. The full thickness of the cortex became positive for the staining by 14 days after birth. Peroxisomes could be detected by electron microscopy in structurally immature proximal tubules in 18-day embryos. Their size increased and the ultrastructure of subcompartments became clear with continuing development of proximal tubules. These results show that peroxisomal enzymes appear in the immature proximal tubules in the kidney of embryos and that the ultrastructure of the peroxisomes and localization of the peroxisomal enzymes develop along with the maturation of proximal tubules and kidney tissues. (J Histochem Cytochem 46:1161-1173, 1998)

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Section: Light Microscopic Immunohistochemistrysupporting

confidence: 84%

Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues

Johkura

Usuda

Liang

et al. 1998

J Histochem Cytochem.

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“…Peroxisomes are surrounded by a single membrane and contain a fine granular matrix (9, 10) (for an historical review on peroxisomes see [11]). De Duve et al (12) coined the term peroxisome for this organelle in 1965 after discovering the enzymes urate and D-amino acid oxidases, both of which produce H 2 O 2 , and catalase, which degrades H 2 O 2 , in the peroxisomal matrix (13).…”

Section: Introductionmentioning

confidence: 99%

Peroxisomes in cardiomyocytes and the peroxisome / peroxisome proliferator-activated receptor-loop

Colasante¹,

Chen²,

Ahlemeyer³

et al. 2015

Thromb Haemost

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SummaryIt is well established that the heart is strongly dependent on fatty acid metabolism. In cardiomyocytes there are two distinct sites for the β-oxidisation of fatty acids: the mitochondrion and the peroxisome. Although the metabolism of these two organelles is believed to be tightly coupled, the nature of this relationship has not been fully investigated. Recent research has established the significant contribution of mitochondrial function to cardiac ATP production under normal and pathological conditions. In contrast, limited information is available on peroxisomal function in the heart. This is despite these organelles harbouring metabolic pathways that are potentially cardioprotective, and findings that patients with peroxisomal diseases, such as adult Refsum´s disease, can develop heart failure. In this article, we provide a comprehensive overview on the current knowledge of peroxisomes and the regulation of lipid metabolism by PPARs in cardiomyocytes. We also present new experimental evidence on the differential expression of peroxisome-related genes in the heart chambers and demonstrate that even a mild peroxisomal biogenesis defect (Pex11α -/-) can induce profound alterations in the cardiomyocyte´s peroxisomal compartment and related gene expression, including the concomitant deregulation of specific PPARs. The possible impact of peroxisomal dysfunction in the heart is discussed and a model for the modulation of myocardial metabolism via a peroxisome/PPAR-loop is proposed.

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“…Whereas peroxisomes in different tissues of normal rodents stain uniformly with the alkaline DABmethod, localization of the activity of several different peroxisomal oxidases with the cerium method and by immunocytochemistry has revealed a strong heterogeneity in enzyme composition within the peroxisomal compartment in a single cell, or in different regions of the same tissue (Angermü ller, 1989;Baumgart, 1997;Fahimi and Baumgart, 1999;Fahimi et al, 1996a;Van den Munckhoff, 1996).…”

Section: Role Of Morphological Techniques In Studying the Peroxisomalmentioning

confidence: 98%

“…However, only a few studies have been carried out to show the specific expression pattern of the corresponding mRNAs in situ (for reviews see: Baumgart, 1997, 2001a. Nonradioactive in situ hybridization using digoxigenin-labeled cRNA-probes on paraffin-embedded rat liver sections, however, revealed that intrahepatic lobular gradients are generated for several "peroxisomal" mRNAs in the liver of rats after treatment with a single dose of the hypolipidemic drug bezafibrate, revealing the distinct transcriptional regulation of the corresponding genes in different parts of the liver lobule (Baumgart et al, 2001a;Schad et al, 1996).…”

Section: Role Of Morphological Techniques In Studying the Peroxisomalmentioning

confidence: 99%

“…In the past few years, our groups have been involved in the establishment of morphological techniques for the localization of peroxisomal proteins and their corresponding mRNAs in different species, tissues, and cell lines (Baumgart 1994(Baumgart , 1997Baumgart et al, 1997;Fahimi et al, 1996b;Fahimi and Baumgart, 1999;Grabenbauer et al, 2001;Kovacs et al, 2001;Schrader et al, 1995). We have applied these techniques for studying fetal and newborn mice in the course of ontogenesis (Grabenbauer et al, 2001) and for the characterization of the phenotype of knockout mice with a peroxisomal biogenesis disorders (PEX5 Ϫ/Ϫ mouse: Baes et al, 1997;; PEX11␤ Ϫ/Ϫ mouse: .…”

Section: Introductionmentioning

confidence: 99%

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A review of morphological techniques for detection of peroxisomal (and mitochondrial) proteins and their corresponding mRNAs during ontogenesis in mice: Application to the PEX5‐knockout mouse with Zellweger syndrome

Baumgart

Fahimi

Steininger

et al. 2003

Microscopy Res & Technique

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In the era of application of molecular biological gene-targeting technology for the generation of knockout mouse models to study human genetic diseases, the availability of highly sensitive and reliable methods for the morphological characterization of the specific phenotypes of these mice is of great importance. In the first part of this report, the role of morphological techniques for studying the biology and pathology of peroxisomes is reviewed, and the techniques established in our laboratories for the localization of peroxisomal proteins and corresponding mRNAs in fetal and newborn mice are presented and discussed in the context of the international literature. In the second part, the literature on the ontogenetic development of the peroxisomal compartment in mice, with special emphasis on liver and intestine is reviewed and compared with our own data reported recently. In addition, some recent data on the pathological alterations in the liver of the PEX5(-/-) mouse with a peroxisomal biogenesis defect are briefly discussed. Finally, the methods developed during these studies for the localization of mitochondrial proteins (respiratory chain complexes and MnSOD) are presented and their advantages and pitfalls discussed. With the help of these techniques, it is now possible to identify and distinguish unequivocally peroxisomes from mitochondria, two classes of cell organelles giving by light microscopy a punctate staining pattern in microscopical immunohistochemical preparations of paraffin-embedded mouse tissues.

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Application of in situ hybridization, cytochemical and immunocytochemical techniques for the investigation of peroxisomes

Cited by 32 publications

References 126 publications

Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues

Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues

Peroxisomes in cardiomyocytes and the peroxisome / peroxisome proliferator-activated receptor-loop

A review of morphological techniques for detection of peroxisomal (and mitochondrial) proteins and their corresponding mRNAs during ontogenesis in mice: Application to the PEX5‐knockout mouse with Zellweger syndrome

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