Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues

Abstract

SUMMARYWe studied the developmental changes in the localization of peroxisome-specific enzymes in rat kidney tissues from embryonic Day 16 to postnatal Week 10 by immunoblot analysis and immunohistochemistry, using antibodies for the peroxisomal enzymes catalase, D-amino acid oxidase, L-␣-hydroxyacid oxidase (isozyme B), and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein. Peroxisomal enzymes were detected in the neonatal kidney by immunoblot analysis and their amount increased with kidney development. By light microscopic immunohistochemistry, they were first localized in a few proximal tubules in the juxtamedullary cortex of 18-day embryos. The distribution of proximal tubules positive for them expanded towards the superficial cortex with development. The full thickness of the cortex became positive for the staining by 14 days after birth. Peroxisomes could be detected by electron microscopy in structurally immature proximal tubules in 18-day embryos. Their size increased and the ultrastructure of subcompartments became clear with continuing development of proximal tubules. These results show that peroxisomal enzymes appear in the immature proximal tubules in the kidney of embryos and that the ultrastructure of the peroxisomes and localization of the peroxisomal enzymes develop along with the maturation of proximal tubules and kidney tissues. (J Histochem Cytochem 46:1161-1173, 1998)

Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

“…While an intense immunoreactivity was present in tubulus proximalis of mice in the control group, maize oil group, normal saline group and Onosma nigricaule group, a moderately intense immunoreactivity was present in the Onosma nigricaule+malathion group and a slightly intense immunoreactivity was determined in the malathion group. Similarly, while CAT immunoreactivity was observed on the tubulus epithelium of internal renal cortex and external renal cortex; there was no staining on the medulla in other studies (2,13,20). In our study, the CAT enzyme activity decreased in the malathion group, compared to other groups.…”

Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases

“…3, j-l). Catalase, which is the most abundant peroxisomal protein, resides in the electron-dense peripheral matrix of peroxisomes (22), and the colocalization observed correlated well with the expected membrane localization of SPoCk-C. Additionally, the pattern of SPoCk-C fluorescence matched the description and abundance of peroxisomes in the tubule as reported by Beard and Holtzman (6). Use of a polyclonal antibody generated for the NH 2 -terminal region of SPoCk-C in wild-type tubules showed a similar staining pattern to those seen in Fig.…”

Physiological functions of D-amino acid oxidases: from yeast to humans

“…Notably, up to now no evidence for the presence of different DAAO isoforms in various tissues of rat (as well as of pig and humans) was reported that should be related to distinct roles of the enzyme. The activity of the renal DAAO increases during the development of rat kidney coordinately with the H 2 O 2 -reducing activity of catalase, and reaches a maximum in 14 -28-day-old rats [119,120]. Recent studies indicated that DAAO could be involved in D-Ser-induced nephrotoxicity due to H 2 O 2 production in the straight part of the proximal tubules where this D-amino acid is readsorbed (Table 1) [121,122].…”