Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

ERDAĞ, BAKIR Buket;

doi:10.1501/vetfak_0000002786

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Catalase is enzyme dispersed in all animal tissues with its highest activity found in red blood cells and the liver. Catalase disintegrates hydrogen peroxide and protects tissues from highly reactive hydroxyl radicals [26]. Catalase activity is reduced during diabetes and this may result in deleterious effects due to the accumulation of superoxide radicals and hydrogen peroxides.…”

Section: Discussionmentioning

confidence: 99%

Balanites aegyptiaca ameliorates insulin secretion and decreases pancreatic apoptosis in diabetic rats: Role of SAPK/JNK pathway

Hassanin

Mahmoud

Hassan

et al. 2018

Biomedicine & Pharmacotherapy

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SAPK-JNK pathway performs a significant role in the pathogenesis of type 2 diabetes. Balanites aegyptiaca (BA) is used as an anti-diabetic agent in folk medicine however its hypoglycemic mechanism is not fully elucidated. The current study aimed to evaluate the effect of crude extract, butanol, and dichloromethane fractions from BA on the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK-JNK) pathway in experimental diabetic rats. Six groups of male Wistar rats were included: normal control, diabetic, diabetic rats treated with crude, butanol or dichloromethane fraction from BA (50 mg/kg BW) and diabetic rats treated with gliclazide as a reference drug for one month. Our results suggested a protective role of treatment of diabetic rats with BA against oxidative stress-induced SAPK-JNK pathway. Moreover, BA treatment produced a reduction in plasma glucose, HbA, lactic acid, lipid profile, malondialdehyde levels and produced an increase in insulin, reduced glutathione levels, catalase and superoxide dismutase activities compared with untreated diabetic rats. Moreover, it decreased apoptosis signal-regulating kinase 1, c-Jun N-terminal kinase 1, protein 53 and increased insulin receptor substrate 1 in rat pancreas while it increased glucose transporter 4 in rat muscle. Analysis of BA extracts by LC-HRMS revealed the presence of different saponins with reported hypoglycemic effect. In conclusion, BA exerted hypoglycemic, hypolipidemic, insulinotropic and antioxidant effects. Additionally, it reduced apoptosis in pancreatic β-cells and increased glucose uptake in muscle. These results suggest that the hypoglycemic effect of BA is due to the inhibition of the SAPK-JNK pathway.

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Section: Discussionmentioning

confidence: 99%

Balanites aegyptiaca ameliorates insulin secretion and decreases pancreatic apoptosis in diabetic rats: Role of SAPK/JNK pathway

Hassanin

Mahmoud

Hassan

et al. 2018

Biomedicine & Pharmacotherapy

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Determination of Acute Toxicity and the Effect of Sub-Lethal Concentration of Quinalphos on Protein and Lipid Peroxidation in <i>Oreochromis niloticus</i>

Shaji,

Abraham,

Mathew

2023

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Quinalphos is an organophosphate used in agriculture that is considered to have potential hazardous effects on nontarget organisms. The present study was designed to determine the median lethal concentration (LC50) of quinalphos (an organophosphate pesticide) on the fish, Oreochromis niloticus, for 96 hours. The LC50 value of quinalphos on Oreochromis niloticus for 96 hours was determined as 3.65 μl/L. The study also focuses on the immediate effect of exposure to sub-lethal concentrations of quinalphos on protein and lipid peroxidation rate after 24 hours in brain and muscle tissues. Protein was found to be decreasing while lipid peroxidation rate increased in both tissues after 24 hours of exposure. By the estimation of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT) activity, it was also identified that quinalphos is capable of impairing the antioxidant defense mechanism of the non-target organism and thereby increase the oxidative stress.

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The effect of ginger essential oil (Zingiber officinale) on catalase in rat kidney tissue

Yıldız Asker,

Aras,

Ölmez

et al. 2024

J Ad VetBio Sci Tech

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We were aimed to investigate the effect of ginger essential oil (Zingiber officinale) on catalase release in rat kidney tissue by histopathological and immunohistochemically method. This study, 21 male Wistar albino rats were used. Rats were divided into three groups: control, 100 mg/kg ginger essential oil (G100), and 500 mg/kg ginger essential oil (G500). Hematoxylin-eosin staining method was used for histopathological evaluations. Immunohistochemically localization of catalase in kidney tissue was determined by streptavidin-biotin peroxidase method. As a result of histopathological evaluations, an increase in glomerulus diameter was observed in kidney tissues of G100 and G500 groups. In addition, vacuolar degeneration was observed in the proximal and distal tubule epithelial cells in the renal cortex of the G100 group. The immunoreactivity of catalase in the renal cortex region; In the control group, it is strong in the proximal tubules and weak in the mesangial cells. While moderate severity in the proximal tubules and weak in the mesangial cells in the G100 group, very weak catalase immunoreactivity was observed in the G500 group. Strong catalase immunoreactivity was detected in the proximal and collecting ducts of the kidney medulla regions of the rats in all groups. We think that ginger essential oil can be used in appropriate doses and times to reduce kidney damage caused by oxidative stress in the kidney.

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Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

Cited by 3 publications

References 18 publications

Balanites aegyptiaca ameliorates insulin secretion and decreases pancreatic apoptosis in diabetic rats: Role of SAPK/JNK pathway

Balanites aegyptiaca ameliorates insulin secretion and decreases pancreatic apoptosis in diabetic rats: Role of SAPK/JNK pathway

Determination of Acute Toxicity and the Effect of Sub-Lethal Concentration of Quinalphos on Protein and Lipid Peroxidation in <i>Oreochromis niloticus</i>

The effect of ginger essential oil (Zingiber officinale) on catalase in rat kidney tissue

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