Reference Values of Lymphocyte Subsets in Healthy, HIV-Negative Children in Cameroon

Sagnia, Bertrand; Ndongo, Francis Atéba; Tetang, Suzie Moyo; Torimiro, Judith; Cairo, Cristiana; Domkam, Irenée; Agbor, Geraldine; Mve, Emmanuel; Tocke, Olive; Fouda, Emilien; Oukem‐Boyer, Odile Ouwe Missi; Colizzi, Vittorio

doi:10.1128/cvi.00483-10

Cited by 29 publications

(31 citation statements)

References 23 publications

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“…A comparison is provided in Tables 3 and 4 of the results obtained from the present study with previously published reference intervals of lymphocyte subsets from children in Hong Kong (8), the United States (6), Cameroon (9), and Italy (7), using similar flow cytometric technologies with monoclonal antibodies. We combined the boys and girls from each age group of the study.…”

Section: Discussionmentioning

confidence: 95%

“…Lymphocyte subset distributions by age are reported in Table 1 (percentages) and Table 2 (absolute counts 310 9 /L), which also list WBC and lymphocyte values. We tabulated the 10th and 90th percentiles, in addition to 11 (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18) 11 (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) 11 (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) …”

Section: Resultsmentioning

confidence: 99%

“…Changes in antibody production are the hallmark of various human B-cell deficiencies, known as primary antibody deficiencies (PADs); these conditions are the most common primary immunodeficiencies (PIDs) (5). Some studies (1,2,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) have demonstrated that age, sex, race, geographic area, and nutritional status can influence variation in Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China lymphocyte subsets, and undernutrition due to insufficient intake of energy and macronutrients and/or due to deficiencies in specific micronutrients can impair the immune system, such as suppressing immune functions that are fundamental to host protection (20), which could in turn influence the distribution of different lymphocyte subsets. Normally, the immune system is functionally immature at birth, and it undergoes sequential development that is genetically determined and additionally stimulated by antigen exposure (21).…”

Section: Introductionmentioning

confidence: 99%

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Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

Jia

Zhang

et al. 2015

Cytometry Pt A

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Immunophenotyping of blood lymphocytes has become an important tool in the diagnosis of immunologic and hematologic disorders such as immunodeficiencies, lymphoproliferative and autoimmune diseases. Lymphocyte subsets include total T-cells, and NK-cells (CD3 2 CD16 1 CD56 1 ). Specific lymphocyte subset reference intervals should be locally established for meaningful comparison and to obtain an accurate interpretation of the results. Reference intervals of lymphocyte subsets for Chinese children are scarce. We performed dual-platform flow cytometry to determine the reference intervals of the percentages and absolute counts of lymphocyte subsets, including total T-cells, TH cells, TC cells, B-cells, and NK-cells in 1,027 ethnic Han children aged 4 months to 7 years in Henan, China. The children were divided into seven age groups. The percentages and absolute counts differed significantly with age, with the percentages of TH cells and B cells and the CD4/CD8 ratio peaking during the first year, while the percentages of total T cells, TC cells, and NK cells were obviously increased with age; girls showed a trend toward having a higher percentage of TH cells and a higher CD4/CD8 ratio than boys. The absolute counts of lymphocyte subsets peaked during first year and then decreased steadily with age. The reference intervals of lymphocyte subsets among children from China differed from the reported values in Hong Kong, the United States, Cameroon, and Italy. The differences observed could be due to genetic and environmental factors, coupled with the methodology used. The reference intervals of lymphocyte subsets could be used as initial national reference ranges in guidelines for children aged 4 months to 7 years. V C 2015 International Society for Advancement of Cytometry

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Section: Discussionmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

Jia

Zhang

et al. 2015

Cytometry Pt A

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“…43 We examined 5 subsets defined by CD56 and CD16 expression (gating strategy is shown in supplemental Figure 2 pos (median 13.5%; P 5 .006) NK cells compared with Nandi children (median 67.25% and 6.07%, respectively). This alteration in relative NK cell subset proportions is illustrated in Figure 1D using representative flow cytometry plots from children within each group.…”

Section: Cd56mentioning

confidence: 99%

Poorly cytotoxic terminally differentiated CD56negCD16pos NK cells accumulate in Kenyan children with Burkitt lymphomas

et al. 2018

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“…CD4 T cell counting was performed in parallel on two different flow cytometers. The first was the FACSCalibur (Becton Dickinson Immunocytometry System [BDIS], San Jose, CA), a dedicated clinical reference flow cytometer for CD4 T cell counting installed at the CIRCB, using Multitest reagent (BDIS) and MultiSet V2.2 software (BDIS) for calculating the absolute and percentage values of CD4 T cells according to the instructions provided by the manufacturer, as previously described (28,29). The second was the Auto40 flow cytometer (Apogee Flow Systems Ltd.) equipped with a green laser at 532 nm, a side scatter detector, two fluorescence channels, and means for direct volumetric counting without requiring a red blood cell lysis step.…”

Section: Methodsmentioning

confidence: 99%

Validation of a Single-Platform, Volumetric, CD45-Assisted PanLeucogating Auto40 Flow Cytometer To Determine the Absolute Number and Percentages of CD4 T Cells in Resource-Constrained Settings Using Cameroonian Patients' Samples

Mbopi-Kéou

Mion

Sagnia

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTThe study evaluated the single-platform, volumetric, CD45-assisted PanLeucogating Auto40 flow cytometer (Apogee Flow Systems Ltd., Hemel Hempstead, United Kingdom) for CD4 T cell numeration, compared to the reference FACSCalibur flow cytometer. Results of absolute counts and percentages of CD4 T cells by Auto40 and FACSCalibur of 234 tripotassium EDTA (K3-EDTA)-blood samples from 146 adults and 88 children (aged from 18 months to 5 years), living in Yaoundé, Cameroon, were highly correlated (r2= 0.97 andr2= 0.98, respectively). The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur absolute CD4 T cell counts were +9.6 cells/μl, with limits of agreement from −251 to 270 cells/μl, and +4.1%, with limits of agreement from −16.1 to 24.4%, respectively. The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur CD4 T cell results expressed as percentages were +0.05% CD4 (95% confidence interval [CI], −0.03 to 0.41), with limits of agreement from −6.0 to 5.9% CD4, and +1.0%, with limits of agreement from −32.3 to 34.4%, respectively. The Auto40 counting allowed identification of the majority of adults with CD4 T cell counts below 200 cells/μl (sensitivity, 87%; specificity, 98%) or below 350 cells/μl (sensitivity, 92%; specificity, 98%) and of children with CD4 T cell counts below 750 cells/μl (sensitivity, 82%; specificity, 98%) or below 25% CD4+(sensitivity, 96%; specificity, 99%). The Auto40 analyzer is a reliable alternative flow cytometer for CD4 T lymphocyte enumeration to be used in routine immunological monitoring according to the WHO recommendations for HIV-infected adults as well as children living in resource-constrained settings.

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Reference Values of Lymphocyte Subsets in Healthy, HIV-Negative Children in Cameroon

Cited by 29 publications

References 23 publications

Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

Poorly cytotoxic terminally differentiated CD56negCD16pos NK cells accumulate in Kenyan children with Burkitt lymphomas

Validation of a Single-Platform, Volumetric, CD45-Assisted PanLeucogating Auto40 Flow Cytometer To Determine the Absolute Number and Percentages of CD4 T Cells in Resource-Constrained Settings Using Cameroonian Patients' Samples

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