Reference material of Prussian blue nanozymes for their peroxidase-like activity

Dong, Haijiao; Wang, Guancheng; Feng, Kaizheng; Wu, Xiaohan; Fan, Yao-Yao; Zhang, Wei; Ma, Ming; Gu, Ning; Zhang, Yu

doi:10.1039/d2an01401g

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…Their application ensures the accuracy and comparability of measurement results across various laboratories, thus providing a reliable framework for evaluating the performance of nanozymes in a standardized manner. 114 This significant accomplishment fills a critical void in the field of nanozymes by establishing reference materials. The newly developed PBNZ reference materials are poised to replace natural HRP and serve as a robust benchmark for evaluating analytical methods and laboratory measurement capabilities.…”

Section: Discussionmentioning

confidence: 92%

Prussian blue nanozymes: progress, challenges, and opportunities

Long

Duan

et al. 2023

Nanoscale

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Section: Discussionmentioning

confidence: 92%

Prussian blue nanozymes: progress, challenges, and opportunities

Long

Duan

et al. 2023

Nanoscale

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“…Our previous studies have provided compelling evidence that the Prussian blue nanozyme (PBNZ) possesses outstanding enzymatic activities, including mimicking catalase (CAT) and superoxide dismutase (SOD), enabling the effective ROS scavenging. Furthermore, we have observed promising preliminary results indicating that the PBNZ contributes to the restoration of macrophage-mediated inflammation resolution. − The encouraging results obtained highlight the PBNZ’s potential as a standalone preresolving agent for alleviating oxidative stress and resolving inflammation within the plaque microenvironment. To further enhance its capacity for modulating the proinflammatory plaque microenvironment, careful design of PBNZ and thoughtful consideration of strategies related to how it activates macrophage-mediated inflammation resolution are crucial.…”

mentioning

confidence: 79%

Design of a Multifunctional Nanozyme for Resolving the Proinflammatory Plaque Microenvironment and Attenuating Atherosclerosis

He,

Han,

Wang

et al. 2023

ACS Nano

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Persistent inflammation within atherosclerotic plaques is a crucial factor contributing to plaque vulnerability and rupture. It has become increasingly evident that the proinflammatory microenvironment of the plaque, characterized by heightened monocyte recruitment, oxidative stress, and impaired clearance of apoptotic cells, plays a significant role in perpetuating inflammation and impeding its resolution. Consequently, targeting and eliminating these proinflammatory features within the plaque microenvironment have emerged as a promising therapeutic approach to restore inflammation resolution and mitigate the progression of atherosclerosis. While recent advancements in nanotherapeutics have demonstrated promising results in targeting individual proinflammatory characteristics, the development of an effective therapeutic strategy capable of simultaneously addressing multiple proinflammatory features remains a challenge. In this study, we developed a multifunctional nanozyme based on Prussian blue,

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“…Stability refers to the capacity of the properties of an RM to remain consistent within predefined values for a specific duration. A longer specified time interval indicates better stability of the property value . Both short- and long-term stability tests were conducted to assess the stability of the RM matrix.…”

Section: Methodsmentioning

confidence: 99%

Development of an Ephedrine In-House Matrix Reference Material and Its Application to Doping Analysis

Kang,

Seo,

Lee

et al. 2024

ACS Omega

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Biomatrix-based reference materials (RMs) improve the quality of laboratory test results by better representing actual samples. However, a matrix RM of ephedrine (EP) for threshold substances that require accurate analysis results has not yet been developed. Therefore, this study aimed to develop an in-house matrix RM for EP and subsequently apply it to analytical procedures. During the development of the inhouse matrix EP RM, the system underwent homogeneity and stability studies. Additionally, it was subjected to interlaboratory comparison study in 11 laboratories, including 10 World Anti-Doping Agency (WADA)-accredited laboratories and our laboratory. Stability testing revealed no significant changes in the RM characteristics. For homogeneity, 10 random batches out of 200 were analyzed to confirm the uniformity within and between bottles. These results, combined with data from 11 laboratories, ensured retroactive validation. The traceability value of the in-house matrix EP RM was assigned as 9.83 ± 0.57 μg/mL (k = 2) by interlaboratory comparison studies and traceable uncertain evaluation. The feasibility of this method as a single calibration standard was confirmed in two laboratories. This substance is reliable and consistent for quality control during EP quantification, ensuring accurate and trustworthy outcomes. Consequently, this study establishes a framework and guidelines for producing in-house matrix RMs and serves as a reference for generating similar matrix RMs in other contexts.

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Reference material of Prussian blue nanozymes for their peroxidase-like activity

Abstract: Nanozymes with their wide applications have rapidly attracted tremendous attention from various fields in the last decade. However, research on the standardization of nanozymes is still lacking. Currently, the accurate...

Cited by 13 publications

References 25 publications

Prussian blue nanozymes: progress, challenges, and opportunities

Prussian blue nanozymes: progress, challenges, and opportunities

Design of a Multifunctional Nanozyme for Resolving the Proinflammatory Plaque Microenvironment and Attenuating Atherosclerosis

Development of an Ephedrine In-House Matrix Reference Material and Its Application to Doping Analysis

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