Reference Baseline Data for Gastric Cytology in Healthy Bottlenose Dolphins (Tursiops truncatus) Under Human Care

Fiorucci, Letizia; Grande, Francesco Dal; Flanagan, Carla; Silva, Joana; Silva, Nuno U.; Sampayo, José; Macrelli, Roberto

doi:10.1578/am.41.3.2015.345

Cited by 3 publications

(11 citation statements)

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“…The mean pH of the gastric samples across all ulcer grades in this study was within the normal range for fasted, clinically healthy dolphins (1.0–3.0, Fiorucci et al, 2015). The results of our study further support Fiorucci et al's (2015) observation that there is no correlation between the pH of gastric fluid and the number of epithelial cells or WBC in the dolphins studied. However, they also found no correlation between the number of epithelial cells and WBC (r 2 = .08), in direct contrast to our observations, which showed that there was a significant correlation between the number of epithelial cells and WBC.…”

Section: Discussionsupporting

confidence: 60%

“…A gastric ulcer is defined as a defect in the wall of the gastric mucosa that extends deep into the gastric submucosa (Yeomans & Naesdal, 2008). The clinical signs of gastric ulcers in humans and other mammals are similar in dolphins and include abdominal tenderness, mental depression, anorexia, inappetence, unresponsiveness, vomiting and regurgitation (Avalos‐Téllez et al, 2010; Fiorucci et al, 2015; C. M. G. Harper et al, 2000). However, diagnosing gastric ulcers based on clinical signs alone can be misleading, since they may also be a sign of other diseases of the stomach, such as gastritis or peptic ulcer disease (Kayacetin & Guresci, 2014; Malfertheiner et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have identified gastric ulcers in the forestomach and occasionally in the main stomach of both wild and captive dolphins (C. M. G. Harper et al, 2000). Ulceration in the pyloric stomach cannot be determined in living dolphins by endoscopy, since the channel connecting the main and pyloric stomachs has a small diameter, is intramural and ‘J’ shaped (Fiorucci et al, 2015; Van Bonn & Dover, 2018). However, ulceration has been reported in the pyloric chambers of Stenella longirostris , Peponocephala electra (Motta et al, 2008) and Stenella frontalis (Suárez et al, 2010) studied through necropsy.…”

Section: Introductionmentioning

confidence: 99%

“…Gastric ulceration has traditionally been diagnosed using one of three methods: (1) radiography, (2) cytology revealing the presence of red blood cells (RBC) and white blood cells (WBC) in the gastric fluid of an individual (Sweeney & Ridgway, 1975) or (3) through endoscopy (termed ‘gastroscopy’). Gastroscopy is preferable to the first two methods since it allows for direct visual examination of the oesophagus, forestomach and entrance of the main stomach via live relayed video imagery (Fiorucci et al, 2015; C. M. G. Harper et al, 2000). A diagnosis of gastric ulcers in the stomach is usually made through gastroscopy when a break in the stomach mucosa >3–5 mm in diameter is observed (Malfertheiner et al, 2009; Yeomans & Naesdal, 2008).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Diagnosing gastric ulcers in bottlenose dolphins (Tursiops sp.) using gastroscopy and cytology

2023

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Gastric ulcers have been reported in a range of cetacean species. Bottlenose dolphins (Tursiops spp.), the most common cetacean species held in captivity, are known to experience gastric ulcers in both wild and captive environments. Documented causes of gastric ulceration include bacterial infection by Helicobacter sp., parasitic infections, high dietary histamine and foreign body ingestion. Gastric ulceration without any obvious cause might be related to stress. Currently, the most accurate way to determine the presence of gastric ulcers in captive dolphins is through direct examination of the stomach mucosa using endoscopy (gastroscopy); a procedure that requires substantial animal training and specialised medical equipment. In this study, we investigate whether cytology of the gastric fluid, collected through the less intensive method of intubation, can be used as an alternative to gastroscopy to predict the presence and severity of gastric ulcers in eight captive bottlenose dolphins at uShaka Sea World, South Africa. An ulcer grading scale was developed to quantify the severity of the dolphins' gastric ulcers observed using gastroscopy. Gastric ulcer severity was then compared with the cytological data collected from gastric fluid samples taken during the gastroscopic examinations. The cytological findings were consistent with other studies, but ulcer severity was not found to be linked to the cytological parameters measured. From these results we suggest that routine cytology of the gastric fluid is not a viable alternative to gastroscopy for diagnosing gastric ulcers in bottlenose dolphins.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Diagnosing gastric ulcers in bottlenose dolphins (Tursiops sp.) using gastroscopy and cytology

2023

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POCUS: Marine Mammals

Lisciandro¹,

Gambino²,

Moore³

et al. 2021

Point‐of‐Care Ultrasound Techniques for the Small Animal Practitioner

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Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases

Segawa

Ohno²,

Tsuchida

et al. 2020

Dis. Aquat. Org.

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Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSBT, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, atpA, and 60 kDa heat-shock protein (hsp60) genes, demonstrated that these isolates form a novel lineage within the genus Helicobacter. Analyses of 16S rRNA, atpA, and hsp60 gene sequences showed that isolate TSBT was most closely related to H. cetorum MIT99-5656T (98.5% similarity), H. pylori ATCC 43504T (76.7% similarity), and H. pylori ATCC 43504T (78.0% similarity), respectively. Type strains of Helicobacter showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name Helicobacter delphinicola sp. nov. with type strain TSBT (= JCM 32789T = TSD-183T). Future studies will confirm whether H. delphinicola plays a role in lesion etiopathogenesis in cetaceans.

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Reference Baseline Data for Gastric Cytology in Healthy Bottlenose Dolphins (Tursiops truncatus) Under Human Care

Cited by 3 publications

References 0 publications

Diagnosing gastric ulcers in bottlenose dolphins (Tursiops sp.) using gastroscopy and cytology

Diagnosing gastric ulcers in bottlenose dolphins (Tursiops sp.) using gastroscopy and cytology

POCUS: Marine Mammals

Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases

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