Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases

Abstract

Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSBT, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, atpA, and 60 kDa heat-shock protein (hsp60) genes, demonstrated that these isolates form a novel lineage within the genus Helicobacter. Analyses of 16S rRNA, atpA, and hsp60 gene sequences showed that isolate TSBT was most closely related to H. cetorum MIT99-5656T (98.5% similarity), H. pylori ATCC 43504T (76.7% similarity), and H. pylori ATCC 43504T (78.0% similarity), respectively. Type strains of Helicobacter showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name Helicobacter delphinicola sp. nov. with type strain TSBT (= JCM 32789T = TSD-183T). Future studies will confirm whether H. delphinicola plays a role in lesion etiopathogenesis in cetaceans.

Helicobacter turcicus sp. nov., a catalase-negative new member of the Helicobacter genus, isolated from Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey

“…canicola [12] are associated with gastroenteritis in human beings [14]. Also, some Helicobacter species in animals may cause gastritis, typhlocolitis, and hepatitis [15–21].…”

“…Nalidixic acid and cephalothin resistance were detected for isolates. The phenotypic characteristics that differentiated 11 isolates from other Helicobacter species are given in Table 3, [3,4,20,21,[58][59][60]. The table contains standard strains close to H. turcicus sp.…”

“…In addition, various enterohepatic species of the Helicobacter genus such as H. cinaedi [5,6], H. canis [7], H. pullorum [8][9][10][11], H. valdiviensis [12], H. winghamensis [13] and H. canicola [12] are associated with gastroenteritis in human beings [14]. Also, some Helicobacter species in animals may cause gastritis, typhlocolitis, and hepatitis [15][16][17][18][19][20][21].…”

Vitamin B12 biosynthesis of Cetobacterium ceti isolated from the intestinal content of captive common bottlenose dolphins (Tursiops truncatus)

“…Despite increasing interest in the comprehensive intestinal microbiota of cetaceans, few studies have investigated the characteristics of each bacterial species in the cetacean intestine. Therefore, our knowledge of the function of cetacean intestinal bacteria is limited to lactic acid bacteria [18,19] and some potential pathogens [11,[20][21][22].…”

Valid publication of new names and new combinations effectively published outside the IJSEM

“…18 The effective publication states that the type strain was also deposited as CCTCC AB2013042, but no documentation was supplied. 19 The effective publication states that the type strain was also deposited as KEMC 224-056, but no documentation was supplied. 20 The authors list GCA 902153315-GCA 902153385 as the genome assemblies, but these appear to be incorrect.…”