ReDuNing, a patented Chinese medicine, reduces the susceptibility to H1N1 influenza of mice loaded with restraint stress

Tang, Lu-Ping; Mao, Zhong-Fu; Li, Xiaoxiao; Chen, Min; Li, Shan-Bing; Tsoi, Bun; Cao, Ling-Fang; Li, Ling; Zeng, Ji-Mei; Wang, Zhiwei; Yu, Guiyuan; Xiao, Wei; Li, Yi-Fang; Kojima, Hiroshi; He, Rong‐Rong

doi:10.1016/j.eujim.2014.07.018

Cited by 22 publications

(14 citation statements)

References 30 publications

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“…Taken together, the antiviral effects of CHA against influenza virus were demonstrated in this study, which agrees with findings of previous studies1723242526. Importantly, CHA could be the potential antiviral material basis of Lonicera japonica Thunb34, Reduning35 and Shuanghuanglian injection36.…”

Section: Discussionsupporting

confidence: 92%

Antiviral activity of chlorogenic acid against influenza A (H1N1/H3N2) virus and its inhibition of neuraminidase

Ding

Cao

et al. 2017

Sci Rep

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151

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Lonicera japonica Thunb, rich in chlorogenic acid (CHA), is used for viral upper respiratory tract infection treatment caused by influenza virus, parainfluenza virus, and respiratory syncytial virus, ect in China. It was reported that CHA reduced serum hepatitis B virus level and death rate of influenza virus-infected mice. However, the underlying mechanisms of CHA against the influenza A virus have not been fully elucidated. Here, the antiviral effects and potential mechanisms of CHA against influenza A virus were investigated. CHA revealed inhibitory against A/PuertoRico/8/1934(H1N1) (EC50 = 44.87 μM), A/Beijing/32/92(H3N2) (EC50 = 62.33 μM), and oseltamivir-resistant strains. Time-course analysis showed CHA inhibited influenza virus during the late stage of infectious cycle. Indirect immunofluorescence assay indicated CHA down-regulated the NP protein expression. The inhibition of neuraminidase activity confirmed CHA blocked release of newly formed virus particles from infected cells. Intravenous injection of 100 mg/kg/d CHA possessed effective antiviral activity in mice, conferring 60% and 50% protection from death against H1N1 and H3N2, reducing virus titres and alleviating inflammation in the lungs effectively. These results demonstrate that CHA acts as a neuraminidase blocker to inhibit influenza A virus both in cellular and animal models. Thus, CHA has potential utility in the treatment of the influenza virus infection.

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Section: Discussionsupporting

confidence: 92%

Antiviral activity of chlorogenic acid against influenza A (H1N1/H3N2) virus and its inhibition of neuraminidase

Ding

Cao

et al. 2017

Sci Rep

Self Cite

151

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“…The remaining groups received oral administration and intraperitoneal injection of water only. On the 1st day of administration, except for normal and virus groups, mice were physically restrained in a 50 mL polypropylene restraint tube with holes for 18 h. After recovering for three days, except for normal group, mice were anesthetized by inhalation of ether vapor and then an approximate 2x LD 50 amount of virus (35 μ L) was instilled into the nares [ 19 , 20 ]. The survival experiments were conducted on 10 mice from each group to observe daily changes in body weight, survival, and several typical symptoms of illness.…”

Section: Methodsmentioning

confidence: 99%

“…Excessive GC caused by increased HPA activity can induce reactive oxygen species (ROS) production and result in inhibition of the susceptibility factors in the initiation of the innate antiviral immune response [ 17 , 18 ]. Previous work from our laboratory has demonstrated that restraint stress increased susceptibility to viral or bacterial pathogens and influenced the severity of infectious disease [ 19 , 20 ]. Moreover, influenza infection was reported to trigger a generalized stress response, leading to a sustained increase in serum GC level and a systemic suppression of host immune responses [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

A New Mechanism of Vitamin C Effects on A/FM/1/47(H1N1) Virus-Induced Pneumonia in Restraint-Stressed Mice

Cai

Tang

et al. 2015

BioMed Research International

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It is well known that vitamin C could protect against influenza infection, but little is known about the mechanisms. This study aimed to investigate the influence and possible mechanisms of vitamin C on pneumonia induced by influenza virus in stressed mice. Results showed that restraint stress significantly increased the mortality and the severity of pneumonia in mice caused by A/FM/1/47(H1N1) virus infection, which was attenuated by oral administration of vitamin C (125 and 250 mg/kg). Moreover, vitamin C administration significantly decreased expression of susceptibility genes, including mitochondrial antiviral signaling (MAVS) and interferon regulatory factor 3 (IRF3), and increased expression of NF-κB. These work in conjunction to induce type I interferons (IFNs) and elicit innate antiviral response as key factors in RIG-I-mediated signal transduction pathway. The above effects of vitamin C were further found to relate with inhibition of excess CORT synthesis by regulating steroid hydroxylating enzymes in adrenal gland. In conclusion, the protective effects of vitamin C on influenza virus-caused pneumonia might be related to its inhibition of CORT synthesis, which reduces the susceptibility to influenza viral infection in restraint-stressed mice. These findings provide a new mechanism for the effects of vitamin C on influenza virus-induced pneumonia in restraint-stressed mice.

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“…It also showed effects in the clinical therapy of influenza and pneumonia. 195,196 Tang and coworkers investigated the protective effects of RDN against influenza and found significant reductions in mortality, mean dead day, and NP gene levels in the lung. Thus, RDN decreased the susceptibility and severity of influenza virus infection in restraint-stressed mice.…”

Section: Anti-influenza Study On Rdnmentioning

confidence: 99%

Development of anti‐influenza agents from natural products

Zhang

Morris‐Natschke

Cheng

et al. 2020

Medicinal Research Reviews

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ReDuNing, a patented Chinese medicine, reduces the susceptibility to H1N1 influenza of mice loaded with restraint stress

Cited by 22 publications

References 30 publications

Antiviral activity of chlorogenic acid against influenza A (H1N1/H3N2) virus and its inhibition of neuraminidase

Antiviral activity of chlorogenic acid against influenza A (H1N1/H3N2) virus and its inhibition of neuraminidase

A New Mechanism of Vitamin C Effects on A/FM/1/47(H1N1) Virus-Induced Pneumonia in Restraint-Stressed Mice

Development of anti‐influenza agents from natural products

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