Reductions of Vibrio parahaemolyticus in Pacific oysters (Crassostrea gigas) by depuration at various temperatures

Phuvasate, Sureerat; Chen, Ming‐Hui; Su, Yi‐Cheng

doi:10.1016/j.fm.2012.02.004

Cited by 42 publications

(32 citation statements)

References 32 publications

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“…However, none of these depuration treatments achieved FDA's targeted 3.52 (MPN/g) log reduction of V. parahaemolyticus for post‐harvest shellfish processing (US Food Drug Administration, ). This agrees with our previous study which reported that depuration at 12.5 °C in 25 L/min did not achieve >3.52 log MPN/g reduction (3.33 log MPN/g; Phuvasate et al, ). Multiple depuration studies have demonstrated significant oyster‐to‐oyster variability, which makes validation of the efficacy of a biologically driven process especially challenging.…”

Section: Resultssupporting

confidence: 93%

Flow rate of depuration system has minimal impact on Vibrio parahaemolyticus decontamination in Pacific oysters (Crassostrea gigas)

Ming

DeWitt

et al. 2018

Journal of Food Safety

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Vibrio parahaemolyticus infections in the United States have been linked to consumption of raw oysters. Depuration has the potential to reduce contamination in live oysters after harvest. This study investigated the impact of depuration flow rate to reduce V. parahaemolyticus in raw oysters. Pacific oysters (n = 35 per trial) were inoculated with a cocktail of V. parahaemolyticus (10290, 10292, 10293, BE 98‐2029, and 027‐1c1) in freshly prepared artificial seawater (70 L). The inoculated oysters were depurated with flow rates of 15, 20, 25, and 35 L/min at 12.5 °C for up to 5 days and V. parahaemolyticus contamination was determined using a three‐tube most probable number (MPN) method. V. parahaemolyticus reductions were as flow rate moderately increased from 15 L/min (2.39 log MPN/g reduction in 5 days) to 35 L/min (3.39 log MPN/g reduction). These results suggest that depuration efficacy can be enhanced by increasing depuration flow rate to 35 L/min. Practical applications Vibrio parahaemolyticus can contaminate raw shellfish, including oysters, during their production and lead to outbreaks of foodborne illness. Depuration, a post‐harvest process, may be used by the shellfish industry to reduce the persistence of V. parahaemolyticus. Previous studies have demonstrated that the depuration process can reduce V. parahaemolyticus in oysters; however, further optimization of the process is necessary to achieve US Food Drug Administration's targeted reduction goal (>3.52 log MPN/g). This study evaluated the impact of depuration flow rate on the reduction of V. parahaemolyticus in Pacific oysters. Increasing flow rates (15–35 L/min) during depuration enhanced the clearance of V. parahaemolyticus in these oysters; however, these conditions were unable to consistently achieve the target of >3.52 log MPN/g reduction. This study provides a reference for the industry on the variability of V. parahaemolyticus in individual oysters and demonstrates that practical modifications (i.e., flow rate) can be implemented in depuration systems to maximize bacterial clearance.

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Section: Resultssupporting

confidence: 93%

Flow rate of depuration system has minimal impact on Vibrio parahaemolyticus decontamination in Pacific oysters (Crassostrea gigas)

Ming

DeWitt

et al. 2018

Journal of Food Safety

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“…However, in the case of bottom held oysters, 48 h was needed to reduce fecal streptococci and E. coli to less than the threshold. Earlier studies have not examined the depuration time with respect to density of animals in the tank (Chae et al 2009, Love et al 2010, Kasai et al 2011, Phuvasate et al 2012). The siphoning activity and filtration capacity of oyster is size dependant and this, too, can affect the time taken for depuration (Jones et al 1991, Oliveira et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Depuration of Bacterial Populations in the Indian Backwater OysterCrassostrea madrasensis(Preston, 1916): Effects on Surface and Bottom Held Oysters

Chinnadurai

Mohamed

Venkatesan

et al. 2014

Journal of Shellfish Research

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“…Depuration is a very effective process for the elimination of faecal bacteria, such as E. coli, but is less effective for naturally occurring Vibrio species [2].Low temperature depuration is recommended by several reports to reduce Vibrio in shellfishes [2,37,38]. Several studies reported that depuration process at ambient temperatures of above 20°C is not effective for eliminating Vibrio spp.…”

Section: Reduction Of Vibrio In Oystersmentioning

confidence: 99%

Evaluation of a Small Scale UV-treated Recirculating Depuration System for Oysters (<i>Crassostrea iredalei</i>)

Sorio¹,

Peralta²

2017

AJFST

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Oysters are filter-feeding organisms that tend to concentrate any suspended materials in its surrounding water including pathogenic bacteria. Since most oysters are eaten as raw or slightly cooked, they can act as vectors for pathogenic microorganisms and thus impose health risks to consumers. Depuration is one of the methods to reduce pathogenic bacteria in oysters to make it safe for sale and consumption. This study was designed to evaluate the effectiveness of the small scale UV-treated recirculating depuration system manipulating different parameters such as water flow rate and tank density. It aims to determine the effect of this UV-treated recirculating depuration system in reducing pathogenic bacteria in oysters such as E. coli, Salmonella, Vibrio cholera and Vibrio parahaemolyticus, and with the survival rate and meat yield of oysters. The experimental results showed that the depuration system was effective in reducing E. coli at different water flow rate (15L/min, 10L/min and 5L/min), in all density level (2 oysters/L, 4 oysters/L and 6 oysters/L). However, for Vibrio parahaemolyticus and Vibrio cholera reduction, only water flow rate of 15L/min at density level of 2 oysters/L and 4 oysters/L revealed to be effective. The survival of the oysters was high in treatments with a density of 2 oysters/ L. The meat yield revealed to have no significant difference (P<0.01) between treatments with water flow rates. In general, treatment with water flow rate of 15 L/min in combination with 2 oysters/L density showed most promise results on all analysis.

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Reductions of Vibrio parahaemolyticus in Pacific oysters (Crassostrea gigas) by depuration at various temperatures

Cited by 42 publications

References 32 publications

Flow rate of depuration system has minimal impact on Vibrio parahaemolyticus decontamination in Pacific oysters (Crassostrea gigas)

Flow rate of depuration system has minimal impact on Vibrio parahaemolyticus decontamination in Pacific oysters (Crassostrea gigas)

Depuration of Bacterial Populations in the Indian Backwater OysterCrassostrea madrasensis(Preston, 1916): Effects on Surface and Bottom Held Oysters

Evaluation of a Small Scale UV-treated Recirculating Depuration System for Oysters (<i>Crassostrea iredalei</i>)

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