Reduction of the hydraulic retention time at constant high organic loading rate to reach the microbial limits of anaerobic digestion in various reactor systems

Ziganshin, Ayrat M.; Schmidt, Thomas; Z, Lv; Liebetrau, Jan; Richnow, Hans H.; Kleinsteuber, Sabine; Nikolausz, Marcell

doi:10.1016/j.biortech.2016.01.096

Cited by 63 publications

(37 citation statements)

References 33 publications

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“…DNA was checked for integrity by agarose gel electrophoresis and quantified with a NanoDrop ND-1000 UV-Vis spectrophotometer (Thermo Fisher Scientific). Amplification of the mcrA genes was carried out with the primers mlas (5′-GGT GGT GTM GGD TTC ACM CAR TA-3′) and mcrA-rev (5′-CGT TCA TBG CGT AGT TVG GRT AGT-3′) using the PCR protocol described previously [16]. …”

Section: Methodsmentioning

confidence: 99%

“…An R script (R version 2.12.2; http://www.r-project.org/) with a cut-off value of six times the standard deviation was used to remove background noise [28]. Multivariate statistical analysis using the vegan package of R (version 3.0.1) was performed on the T-RFLP profiles applying the Bray-Curtis dissimilarity index [29] as described previously [16, 22]. Correlations between the abundance of different T-RF and various reactor parameters were analyzed with the R Hmisc package (based on Spearman's rank correlation coefficient).…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, an increased activity of Methanosaeta spp. was discovered during shortening the hydraulic retention time (HRT) despite the high levels of organic acids, a result which was additionally supported by stable isotope fingerprinting of the biogas [15, 16]. Different hydrogenotrophic methanogens can form close associations with syntrophic acetate-oxidizing bacteria (SAOB; e.g., Clostridium ultunense [17], Syntrophaceticus schinkii [18], and Tepidanaerobacter acetatoxydans [19]) at elevated temperatures or high ammonia levels.…”

Section: Introductionmentioning

confidence: 99%

“…However, the rRNA approach applied in our previous work does not completely reflect the actual community composition of methanogenic archaea due to its lower phylogenetic resolution [15, 16, 23]. Moreover, the relative abundance data obtained by 16S rRNA gene analysis is biased due to the different copy numbers of rRNA operons in various methanogenic taxa (e.g., Methanoculleus : 1 copy; Methanosaeta : 1-2 copies; Methanococcus : 2–4 copies; Methanosarcina : 3 copies; Methanospirillum : 4 copies according to https://rrndb.umms.med.umich.edu/) [24], while mcrA is a single-copy gene in most methanogens (in rare cases, an additional mrt gene encoding an isoenzyme is found in members of Methanobacteriales and Methanococcales).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Ziganshin

Ziganshina

Kleinsteuber

et al. 2016

Archaea

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Comparative analysis of methanogenic archaea compositions and dynamics in 11 laboratory-scale continuous stirred tank reactors fed with different agricultural materials (chicken manure, cattle manure, maize straw, maize silage, distillers grains, and Jatropha press cake) was carried out by analysis of the methyl coenzyme-M reductase α-subunit (mcrA) gene. Various taxa within Methanomicrobiales, Methanobacteriaceae, Methanosarcinaceae, Methanosaetaceae, and Methanomassiliicoccales were detected in the biogas reactors but in different proportions depending on the substrate type utilized as well as various process parameters. Improved coverage and higher taxonomic resolution of methanogens were obtained compared to a previous 16S rRNA gene based study of the same reactors. Some members of the genus Methanoculleus positively correlated with the relative methane content, whereas opposite correlations were found for Methanobacterium. Specific biogas production was found to be significantly correlating with Methanosarcinaceae. Statistical analysis also disclosed that some members of the genus Methanoculleus positively correlated with the ammonia level, whereas the prevalence of Methanocorpusculum, Methanobacterium, and Methanosaeta was negatively correlated with this parameter. These results suggest that the application of methanogenic archaea adapted to specific feedstock might enhance the anaerobic digestion of such waste materials in full-scale biogas reactors.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Ziganshin

Ziganshina

Kleinsteuber

et al. 2016

Archaea

Self Cite

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“…Some organic residues, such as industrial, municipal or agricultural wastewaters, liquid manures and side-streams of other biological conversion or down-streaming processes, as well as alternative feedstocks such as harvested suspensions from microalgae production, are characterized by high water and low total solids content. In continuously stirred tank reactors this leads to either high reactor volume requirements or low hydraulic retention times which can result in wash-out of slowly growing microbes, and induce process instabilities [41,167]. An effective solution to prevent microbial wash-out is the retention and enrichment of active biomass within the reactor by sedimentation, retention of self-aggregated granules, or immobilization through biofilm growth on carrier materials [104].…”

Section: Engineering For An Increased Use Of Residues and Novel Feedsmentioning

confidence: 99%

The Future Agricultural Biogas Plant in Germany: A Vision

et al. 2019

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After nearly two decades of subsidized and energy crop-oriented development, agricultural biogas production in Germany is standing at a crossroads. Fundamental challenges need to be met. In this article we sketch a vision of a future agricultural biogas plant that is an integral part of the circular bioeconomy and works mainly on the base of residues. It is flexible with regard to feedstocks, digester operation, microbial communities and biogas output. It is modular in design and its operation is knowledge-based, information-driven and largely automated. It will be competitive with fossil energies and other renewable energies, profitable for farmers and plant operators and favorable for the national economy. In this paper we discuss the required contribution of research to achieve these aims.

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Microbial Interactions in Anaerobic Wastewater Treatment

Wang

2019

Encyclopedia of Water

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Anaerobic treatment technology, represented by anaerobic digestion, is an important microbial process for wastewater treatment. Anaerobic treatment is superior to aerobic processes with reduced energy demand, lower excess sludge production, and the generation of methane as a source of renewable energy. In anaerobic treatment, organic waste is converted into methane and carbon dioxide via four successive steps – hydrolysis, acidogenesis, acetogenesis, and methanogenesis. The interactions between microbial populations have major impacts on the performance of anaerobic treatment processes. The competition between populations of Firmicutes and Bacteroidetes is closely correlated to process stability. Similarly, the competition between two acetoclastic methanogens with distinct kinetics characteristics, i.e. Methanosaeta and Methanosarcina , is linked to changes in the level of acetate as a metabolic intermediate associated with process perturbations. Further, syntrophic interactions between the Bacteria and Archaea play central roles in maintaining process balance. Indeed, syntrophic interactions are required for the conversion of diverse intermediates and substrates, including organic acids, alcohols, and aromatics, in anaerobic treatment. The microbial interactions underlying anaerobic treatment are critical to process performance. The availability of new tools, such as high‐throughput sequencing technologies, makes it possible to gain further understanding of population interactions at the microbiome level in order to develop more robust anaerobic treatment processes for broader applications as sustainable alternatives.

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Reduction of the hydraulic retention time at constant high organic loading rate to reach the microbial limits of anaerobic digestion in various reactor systems

Cited by 63 publications

References 33 publications

Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

The Future Agricultural Biogas Plant in Germany: A Vision

Microbial Interactions in Anaerobic Wastewater Treatment

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