Reduction of Salmonella on chicken meat and chicken skin by combined or sequential application of lytic bacteriophage with chemical antimicrobials

Sukumaran, Anuraj T.; Nannapaneni, Ramakrishna; Kiess, Aaron S.; Sharma, Chander Shekhar

doi:10.1016/j.ijfoodmicro.2015.04.025

Cited by 102 publications

(71 citation statements)

References 35 publications

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“…S. Rissen was processed as reported by Sukumaran et al 53 For the test, 250 g of chicken minced meat was divided into five equal parts. Four sections were infected with S. Rissen (10 3 CFU/mL) and incubated at room temperature according to ISO 16140-2: 2016; 54 while the last one was treated only with PBS (negative control).…”

Section: Bacterial Reduction Assay On Chicken Minced Meatmentioning

confidence: 99%

Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

Fulgione

Ianniello

Papaianni

et al. 2019

IJN

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The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bacteriophages. The final goal of this study was to demonstrate that biomimetic HA nanocrystals can be used for bacteriophage delivery in the context of bacterial infections, and contribute-at the same time-to enhance some of the biological properties of the same bacteriophages such as stability, preservation, antimicrobial activity, and so on. Materials and methods: Phage isolation and characterization were carried out by using Mitomycin C and following double-layer agar technique. The biomimetic HA water suspension was synthesized in order to obtain nanocrystals with plate-like morphology and nanometric dimensions. The interaction of phages with the HA was investigated by dynamic light scattering and Zeta potential analyses. The cytotoxicity and intracellular killing activities of the phage-HA complex were evaluated in human hepatocellular carcinoma HepG2 cells. The bacterial inhibition capacity of the complex was assessed on chicken minced meat samples infected with Salmonella Rissen. Results: Our data highlighted that the biomimetic HA nanocrystal-bacteriophage complex was more stable and more effective than phages alone in all tested experimental conditions. Conclusion: Our results evidenced the important contribution of biomimetic HA nanocrystals: they act as an excellent carrier for bacteriophage delivery and enhance its biological characteristics. This study confirmed the significant role of the mineral HA when it is complexed with biological entities like bacteriophages, as it has been shown for molecules such as lactoferrin.

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Section: Bacterial Reduction Assay On Chicken Minced Meatmentioning

confidence: 99%

Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

Fulgione

Ianniello

Papaianni

et al. 2019

IJN

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“…Chibeu et al (2013) demonstrated that the combination of the bacteriophage preparation Listex TM P100 with the chemical compounds potassium lactate and sodium diacetate resulted in an improvement in their action against bacteria present on the surface of Ready to eat (RTE) roast beef and cooked turkey when compared with each agent alone (Chibeu et al 2013). Also, Sukumaran et al (2015) showed that the combined or sequential application of the bacteriophage preparation SalmoFresh TM with lauric arginate or cetylpyridinum chloride was beneficial in reducing Salmonella on chicken meat and chicken skin.…”

Section: Discussionmentioning

confidence: 99%

Control ofSalmonellaEnteritidis on food contact surfaces with bacteriophage PVP-SE2

et al. 2018

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Salmonella is one of the worldwide leading foodborne pathogens responsible for illnesses and hospitalizations, and its capacity to form biofilms is one of its many virulence factors. This work evaluated (bacterio)phage control of adhered and biofilm cells of Salmonella Enteritidis on three different substrata at refrigerated and room temperatures, and also a preventive approach in poultry skin. PVP-SE2 phage was efficient in reducing both 24-and 48-h old Salmonella biofilms from polystyrene and stainless steel causing 2 to 5 log CFU cm À2 reductions with a higher killing efficiency at room temperature. PVP-SE2 phage application on poultry skins reduced levels of Salmonella. Freezing phage-pretreated poultry skin samples had no influence on the viability of phage PVP-SE2 and their in vitro contamination with S. Enteritidis provided evidence that phages prevented their further growth. Although not all conditions favor phage treatment, this study endorses their use to prevent and control foodborne pathogen colonization of surfaces.

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“…Interventions using bacteriophages can be performed at different or even multiple points in the food processing facility or throughout the food chain to enhance the control process. It has been suggested that Salmonella bacteriophages can be added by dipping or spraying or as a liquid to different food matrices (Spricigo et al, 2013;Zinno et al, 2014;Bao et al, 2015;Sukumaran et al, 2015).…”

Section: As Bio-control Agents In Food Industrymentioning

confidence: 99%

Bacteriophages and their applications

Thung¹,

Lee²,

Premarathne³

et al. 2018

Food Res.

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Bacteriophages are ubiquitous in our world, mainly in the oceans, soil, the water and food we consume. They can be used efficiently in modern biotechnology, as well as alternatives to antibiotics for many antibiotic resistant bacterial strains. Phages can be used as vehicles for vaccines both DNA and protein, for the detection of pathogenic bacterial strain, as biocontrol agents in agriculture and food industry. This review outlines the properties as well as the influence of different external physical and chemical factors like temperature and acidity on phage persistence. A better understanding of the complex problem of phage sensitivity to external factors may be useful for other researchers working with phages. Furthermore, the applications of bacteriophages were described in this paper as well.

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Reduction of Salmonella on chicken meat and chicken skin by combined or sequential application of lytic bacteriophage with chemical antimicrobials

Cited by 102 publications

References 35 publications

<p>Biomimetic hydroxyapatite nanocrystals are an active carrier for <em>Salmonella</em> bacteriophages</p>

<p>Biomimetic hydroxyapatite nanocrystals are an active carrier for <em>Salmonella</em> bacteriophages</p>

Control ofSalmonellaEnteritidis on food contact surfaces with bacteriophage PVP-SE2

Bacteriophages and their applications

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