Reduction of Postincisional Allodynia by Subcutaneous Bupivacaine

Duarte, Adriana M.; Pospíšilová, Eva; Reilly, Erin; Mujenda, Florence H.; Hamaya, Yoshihiro; Strichartz, Gary R.

doi:10.1097/00000542-200507000-00018

Cited by 88 publications

(25 citation statements)

References 35 publications

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“…Incisions on the back increased the local responses to tactile punctate stimulation with von Frey filaments, as reported previously (Duarte et al, 2005). Primary and secondary hyperesthesia were apparent at the first testing, 0.5 hour after incision, (Figs.…”

Section: Tactile Sensitivity and Post-incisional Hyperesthesia Of Haisupporting

confidence: 86%

“…We and others have previously shown that central sensitization appears after incision in rat hairy skin, as evidenced by the actions of systemic local anesthetics on post-incisional pain (Duarte et al, 2005) and by recordings from high threshold and low threshold afferents as well as wide dynamic range (WDR) spinal cord neurons (Kawamata et al, 2005). During incision and suturation an increase in activity was recorded in all three classes of spinal neurons, but spontaneous activity and that evoked by light tactile stimulation, as is used to assess allodynia, for several hours after surgery was only prominent in the WDR neurons (Kawamata et al, 2005).…”

Section: Discussionmentioning

confidence: 81%

“…For each force, at the specified distances, the normalized scores of all rats were averaged at each time point, beginning with the pre-treatment baseline taken over 5-6 days before incision (intact skin), and at 0.5, 1, 1.5, 2, 3, 4, 8 and 24 hours after treatment (Duarte et al, 2005). Post-incisional and dose-dependent BQ-123 effects were statistically evaluated with the Mann-Whitney U-Test (Statview, Inc., Cary, N.C.).…”

Section: Data Normalization and Analysismentioning

confidence: 99%

“…BQ-123 was diluted in phosphate buffered saline (PBS, pH=6.8) to match the conditions we had described in an earlier description of this incision model (Duarte et al, 2005). To minimize local skin irritations by the time of surgery, all rats were clipped on the day preceding the treatment.…”

Section: Drugs and Surgerymentioning

confidence: 99%

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Cutaneous endothelin-A receptors elevate post-incisional pain

Mujenda

Duarte

Reilly

et al. 2007

Pain

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The contribution of endothelin-1 (ET-1), acting via endothelin-A receptors (ET A ), on post-incisional pain was examined in a rat model of incision through the hairy skin of the lumbar dorsum. Postincisional mechanical hyperesthesia was evaluated by cutaneous trunci muscle reflexes (CTMR) of subcutaneous muscles responding to stimulation with von Frey filaments near the wound (primary responses) and at a distance, especially on the contralateral dorsum (secondary responses, involving spinal circuits). The role of ET A was determined by pre-incisional, subcutaneous injection of the selective receptor antagonist BQ-123 at the incision site, 15 min or 24 hr before surgery. Control incisions showed both primary tactile allodynia and hyperalgesia, and a weaker secondary hyperesthesia, peaking 3-4 h after surgery and lasting at least 24h. Primary allodynia, but not hyperalgesia, was dose-dependently suppressed by 15 min pre-incisional BQ-123. In contrast, both secondary allodynia and hyperalgesia were inhibited by local BQ-123. The suppression of primary allodynia by local antagonist disappeared in 24 h, but that of secondary hyperesthesia remained strong for at least 24 h. Systemically delivered BQ-123 was without effect on any post-incisional hyperesthesia, and if the antagonist was locally injected 24 h before surgery there was no difference on hyperesthesia compared to vehicle injected at that time. We conclude that ET-1, released from skin by incision, activates nociceptors to cause primary allodynia and to sensitize spinal circuits through central sensitization. Blockade of ET A in the immediate peri-operative period prevents the later development of central sensitization.

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Section: Tactile Sensitivity and Post-incisional Hyperesthesia Of Haisupporting

confidence: 86%

Section: Discussionmentioning

confidence: 81%

Section: Data Normalization and Analysismentioning

confidence: 99%

Section: Drugs and Surgerymentioning

confidence: 99%

See 2 more Smart Citations

Cutaneous endothelin-A receptors elevate post-incisional pain

Mujenda

Duarte

Reilly

et al. 2007

Pain

View full text Add to dashboard Cite

show abstract

“…Second, despite the fundamental differences in mechanism of action, basic science investigations suggest that both local anesthetic agents and Cox inhibitors decrease peripheral and central sensitization via a direct central nervous system effect [12,13]. When administered systemically to decerebrate animals, local anesthetics decrease dorsal horn neuronal excitability [12,14]. In addition, it is possible that repeated local anesthetic wound instillation decreases injury-induced C-fiber activity with a resultant decrease in peripheral and central sensitization [13].…”

Section: Discussionmentioning

confidence: 99%