Reduction of Phenolindo-2,6-dichlorophenol in Dark and Light by the Blue-Green Alga, Anabaena variabilis

1. The biliproteins C-phycocyanin and allophycocyanin were purified from the blue-green alga Anabaena variabilis by ammonium sulphate fractionation and gel filtration. 2. An assay procedure that enabled the proportion of the two pigments, present as a mixture, to be determined was devised by using the data provided by spectrophotometric analysis of the purified biliproteins. 3. The degree of association and relative proportions of the two pigments were analysed by the application of this procedure to the components separated by thin-layer gel filtration. 4. The C-phycocyanin/allophycocyanin ratio remained essentially constant in algal extracts prepared at various stages throughout the growth cycle or after growth under conditions of reduced illumination. 5. The behaviour of the C-phycocyanin aggregate species from Anacystis nidulans suggested that they were of appreciably lower molecular weight than those observed in extracts of Anabaena variabilis.

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“…Organisms. The blue-green algae used (Anabaena variabilis Kutzing and Anacy8tis nidulans) were maintained as described by Carr & Hallaway (1965).…”

Section: Methodsmentioning

confidence: 99%

C-phycocyanin and allophycocyanin in two species of blue-green algae

Craig

Carr

1968

Biochemical Journal

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“…Growth of Anabaena variabilis. The organism was grown photosynthetically on the mineral salt medium C of Kratz & Myers (1955) as previously described (Carr & Hallaway, 1965) and growth was monitored by measuring the extinction of the culture at 650 nm in a Unicam SP600 spectrophotometer. Under the condition employed the extinction was proportional to cell dry weight and an extinction of 1.0 at 650 nm wavelength corresponded to 0.36 g dry wt of organism/l of culture.…”

Section: E T H O D Smentioning

confidence: 99%

In vitro Protein Synthesis and Measurement of the Stability of Messenger RNA in the Blue-green Alga, Anabaena variabilis

Leach

Carr

2000

Microbiology

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A cell-free preparation of the blue-green alga, Anabaena variabilis, that incorporates W-labelled amino acids into protein has been prepared and characterized. The activation of amino acids to amino acid transfer RNAs was characterized, and assembly of these into peptides has been described with respect to cofactor requirements and antibiotic sensitivity. The properties of these systems and the effect of antibiotics on them are similar to those of bacteria. Both natural and synthetic messenger RNA were effective in peptide formation. The kinetics of incorporation of [Wluracil into RNA has been examined and the stability of labelled RNA from A . variabilis measured by radioactivity loss and by its role in directing peptide synthesis. The half-life of messenger RNA from this organism is approximately 12 min, and as such is comparable to that of bacteria when based upon the mean generation time of the respective organisms.

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“…The blue-green alga Anacystis nidulans has also have observed endogenous {dark) dye been used extensively for photosynthetic reduction in blue-green algae (3,6), but the measurements using the light-dependent re-rate and extent of reduction of DPIP in these duction of various dyes, including the electro-reports, as well as for other blue-greens tested positive compound 2,6-dichlorophenolindophe-in our laboratory are severalfold less than no1 (DPIP) (E,' = 0.224 V). Although intact that exhibited by Anacystis.…”

mentioning

confidence: 99%

The non-light-dependent reduction of 2,6-dichlorophenolindophenol by cells of the blue-green alga Anacystis nidulans

Ward¹

1975

Can. J. Microbiol.

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Whole cells of the blue-green alga Anacystis nidulans reduced, in the dark, the oxidation-reduction dye, 2,6-dichlorophenolindophenol at rates severalfold higher than those of the other algae tested. Under anaerobiosis, the endogenous reductant was depleted after up to 80 nmol of dye were reduced per microliter of cells. Cells held in darkness for several hours exhibited lowered dark reduction rates relative to cells held in light. Treatment with lysozyme and ethylenediaminetetraacetic acid yielded cells that would photoreduce the dye, whereas untreated cells would not. Comparisons of photoreduction and dark reduction revealed that the dark reduction proceeded independently of the photoreduction. It was concluded that the dark reduction represents a pool of endogenous reductant of sufficiently low oxidation-reduction potenital to reduce completely 2,6-dichlorophenolindophenol. Additionally, untreated cells were shown to be permeable to the dye although they did not photoreduce it; thus lysozyme/ethylenediaminetetraacetic acid treatment was considered to make the oxidant accessible to the photosynthetic machinery.

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Reduction of Phenolindo-2,6-dichlorophenol in Dark and Light by the Blue-Green Alga, Anabaena variabilis

Cited by 20 publications

References 10 publications

C-phycocyanin and allophycocyanin in two species of blue-green algae

C-phycocyanin and allophycocyanin in two species of blue-green algae

In vitro Protein Synthesis and Measurement of the Stability of Messenger RNA in the Blue-green Alga, Anabaena variabilis

The non-light-dependent reduction of 2,6-dichlorophenolindophenol by cells of the blue-green alga Anacystis nidulans

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