Reduction of periodontal pathogens adhesion by antagonistic strains

Hoogmoed, van Christianus; Geertsema-Doornbusch, Geessien; Teughels, Wim; Quirynen, Marc; Busscher, Henk J.; Mei, van der Henny C.

doi:10.1111/j.1399-302x.2007.00388.x

Cited by 66 publications

(65 citation statements)

References 31 publications

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“…There was no difference in the number of cultivated or as-yet-uncultivated organisms between the two groups (p > 0.05, two-sample t test on transformed data) (AYS, HNN, PSK) Shiloah et al, 2000;Haffajee and Socransky, 2001;van Winkelhoff et al, 2001;Van der Velden et al, 2003;. …”

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confidence: 79%

Subgingival Microbial Profiles of Smokers with Periodontitis

2010

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The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.

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confidence: 79%

Subgingival Microbial Profiles of Smokers with Periodontitis

2010

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“…S. sanguinis is commonly found in the oral cavity, is not considered pathogenic, and may be beneficial to the host. This bacterium is thought to demonstrate a competitive inhibition of S. mutans, 24,25 whereas S. mutans is closely related to the pathogenesis of caries. 22 F. nucleatum, a gram-negative anaerobic rod, is an important link between the primary and secondary colonizers in the dental plaque and is detected in chronic periodontitis lesions and frequently recovered from patients with necrotizing-ulcerative gingivitis and/or periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Efficacy of 3 Oral Antiseptics Containing Octenidine, Polyhexamethylene Biguanide, or Citroxx: Can Chlorhexidine Be Replaced?

Rohrer

Widmer

Waltimo

et al. 2010

Infect. Control Hosp. Epidemiol.

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Background.Use of oral antiseptics decreases the bacterial load in the oral cavity.Objective.To compare the antimicrobial activity of 3 novel oral antiseptics with that of Chlorhexidine, which is considered the “gold standard” of oral hygiene.Design.Comparative in vitro study.Methods.Four common oral microorganisms (Streptococcus sanguinis, Streptococcus mutans, Candida albicans, andFusobacterium nucleatum) were tested under standard conditions and at different concentrations, by use of a broth dilution assay and an agar diffusion assay and by calculating the log10reduction factor (RF). The antimicrobial activity of each antiseptic was assessed by counting the difference in bacterial densities (ie, the log10number of colony-forming units of bacteria) before and after the disinfection process.Results.The oral antiseptics containing octenidine (with an RF in the range of 7.1–8.24 CFU/mL) and polyhexamethylene biguanide (with an RF in the range of 7.1–8.24 CFU/mL) demonstrated antimicrobial activity comparable to that of Chlorhexidine (with an RF in the range of 1.03–8.24 CFU/mL), whereas the mouth rinse containing Citroxx (Citroxx Biosciences; with an RF in the range of 0.22–1.36 CFU/mL) showed significantly weaker antimicrobial efficacy. Overall, octenidine and polyhexamethylene biguanide were more active at lower concentrations.Conclusion.Oral antiseptics containing the antimicrobial agent octenidine or polyhexamethylene biguanide may be considered as potent alternatives to chlorhexidine-based preparations.

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“…The microbial communities of the oral cavity harbor hundreds of bacterial phylotypes and intense interspecies competition exists between bacteria that depend on the limited nutrients available in these biofilms (8,35,38,52). The interactions between oral commensals and oral patho- FIG.…”

Section: Discussionmentioning

confidence: 99%

The EIIAB^ManPhosphotransferase System Permease Regulates Carbohydrate Catabolite Repression inStreptococcus gordonii

Tong

Zeng

Burne

2011

Appl Environ Microbiol

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Commensal oral streptococci play critical roles in oral biofilm formation and promote dental health by competing with, and antagonizing the growth of, pathogenic organisms, such as Streptococcus mutans. Efficient utilization of the spectrum of carbohydrates in the oral cavity by commensal streptococci is essential for their persistence, and yet very little is known about the regulation of carbohydrate catabolism by these organisms. Carbohydrate catabolite repression (CCR) in the abundant oral commensal Streptococcus gordonii strain DL-1 was investigated using the exo-␤-D-fructosidase gene (fruA) and a fructose/mannose sugar:phosphotransferase (PTS) enzyme II operon (levDEFG) as model systems. Functional studies confirmed the predicted roles of FruA and LevD in S. gordonii. ManL, the AB domain of a fructose/mannose-type enzyme II PTS permease, contributed to utilization of glucose, mannose, galactose, and fructose and exerted primary control over CCR of the fruA and levD operons. Unlike in S. mutans, ManL-dependent CCR was not sugar specific, and galactose was very effective at eliciting CCR in S. gordonii. Inactivation of the apparent ccpA homologue of S. gordonii actually enhanced CCR of fruA and levD, an effect likely due to its demonstrated role in repression of manL expression. Thus, there are some similarities and fundamental differences in CCR control mechanisms between the oral pathogen S. mutans and the oral commensal S. gordonii that may eventually be exploited to enhance the competitiveness of health-associated commensals in oral biofilms.

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Reduction of periodontal pathogens adhesion by antagonistic strains

Abstract: The adhesion of P. gingivalis was inhibited best by antagonistic strains, while S. mitis BMS appeared to be the most successful antagonist.

Cited by 66 publications

References 31 publications

Subgingival Microbial Profiles of Smokers with Periodontitis

Subgingival Microbial Profiles of Smokers with Periodontitis

Antimicrobial Efficacy of 3 Oral Antiseptics Containing Octenidine, Polyhexamethylene Biguanide, or Citroxx: Can Chlorhexidine Be Replaced?

The EIIAB^ManPhosphotransferase System Permease Regulates Carbohydrate Catabolite Repression inStreptococcus gordonii

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Reduction of periodontal pathogens adhesion by antagonistic strains

Abstract: The adhesion of P. gingivalis was inhibited best by antagonistic strains, while S. mitis BMS appeared to be the most successful antagonist.

Cited by 66 publications

References 31 publications

Subgingival Microbial Profiles of Smokers with Periodontitis

Subgingival Microbial Profiles of Smokers with Periodontitis

Antimicrobial Efficacy of 3 Oral Antiseptics Containing Octenidine, Polyhexamethylene Biguanide, or Citroxx: Can Chlorhexidine Be Replaced?

The EIIABManPhosphotransferase System Permease Regulates Carbohydrate Catabolite Repression inStreptococcus gordonii

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The EIIAB^ManPhosphotransferase System Permease Regulates Carbohydrate Catabolite Repression inStreptococcus gordonii