Reduction of paraquat toxicity in maize leaves by benzyladenine

Durmus, Nuran; Kadıoğlu, Asim

doi:10.1556/abiol.56.2005.1-2.10

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…This suggested that the reduced production of H 2 O 2 in the presence of BA supplementation might be the result of increased peroxidase activity more than catalase. Such a protective effect of BA against oxidative stress has been reported previously and explained as BA affecting the activity of POX (Durmuş and Kadioğlu 2005). The presence of AgNPs in the seed culture medium at a low concentration (1 mg L −1 ) improved the impact of BA on the activities of microshoot antioxidant enzymes, but higher doses of AgNPs (2.5 and 5 mg L −1 ) considerably reduced POX activity in propagated shoots.…”

Section: Resultssupporting

confidence: 72%

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Bernard

Moghadam

Mirzajani

2015

In Vitro Cell.Dev.Biol.-Plant

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Hyperhydricity syndrome (HHS) occurs when plants are stressed resulting in sharp changes in the ethylene concentration in the in vitro culture. One of the characteristics of HHS is the reduction of cell wall lignification. The aim of this study was to examine the effects of silver nanoparticles (AgNPs) on HHS in micropropagated Thymus daenensis shoots. AgNPs (0, 1, 2.5, or 5 mg L −1 ) were mixed with Murashige and Skoog (MS) culture medium and the antioxidant response and variations in lignin content were measured on micropropagated shoots derived from seedlings cultured with AgNPs. In addition, the different forms of microshoot polyamines (PAs) were investigated through High Performance Thin Layer Chromatography (HPTLC). The results indicated that seedling pretreatment with AgNPs at concentrations of 1 and 2.5 mg L −1 led to a decrease in the activity of the microshoot antioxidant system resulting in an increase of H 2 O 2 . Furthermore, the increased lignin content in response to the 2.5 mg L −1 AgNPs treatment was accompanied by an increase in the concentration of the bonded form of putrescine (Put) and spermine (Spm). There was a direct correlation between the lignin content and the concentration of bonded PAs. We hypothesize that the higher level of bonded PAs reduced the concentration of the free forms, leading to increased lignification. This may have led to the decreased HHS observed in shoot cultures.

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Section: Resultssupporting

confidence: 72%

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Bernard

Moghadam

Mirzajani

2015

In Vitro Cell.Dev.Biol.-Plant

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“…in the present study, sod activity significantly increased in the leaves treated with diuron for, respectively, 48 and 72 h. similarly, increases in sod activity have been observed in response to treatment with herbicides paraquat, diquat, diuron, atrazine and prometryne [17,18,24,33]. In the present study, the effects of polyamines on sod activity were different according to the polyamine tested and time after diuron treatment.…”

Section: Discussionsupporting

confidence: 76%

Pretreatment with polyamines alleviate the deleterious effects of diuron in maize leaves

Durmus¹,

Bekircan²

2015

Acta Biologica Hungarica

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the effects of diuron, a photosystem ii inhibiting herbicide, on lipid peroxidation, photosynthetic pigments, soluble protein, proline contents and some antioxidant enzymes in maize leaves were studied and protective effects of polyamines against diuron toxicity were investigated. Diuron significantly increased lipid peroxidation, suggesting oxidative damage in the plants and proline content, while it decreased total chlorophyll, carotenoid and soluble protein levels in the leaves during the experiments. Pretreatment with polyamines statistically decreased lipid peroxidation induced by diuron and spermine (sPm) proved to be the most effective polyamine. Also, pretreatment with polyamines significantly prevented the losses of total chlorophyll, carotenoid and soluble protein induced by diuron. on the other hand, pretreatment with polyamines significantly increased proline contents of the leaves in comparison with the leaves treated with diuron. superoxide dismutase (sod), guaiacol peroxidase (GPX) and glutathione reductase (Gr) activities increased in the leaves treated with diuron while catalase (cat) activity decreased. Pretreatment with spermidine (SPD) did not change significantly SOD activity at 24 and 72 hrs of diuron treatment but prevented the increase in sod activity induced by diuron at 48 h. however, pretreatment with sPd increased GPX activity at 24 h and GR activity at 48 and 72 hrs. CAT activity in the leaves pretreated with SPD was similar to that of the leaves treated with diuron. Pretreatment with sPm prevented the increase in sod activity induced by diuron at 48 h but significantly increased it at 72 h of diuron treatment. However, pretreatment with SPM did not significantly change GPX and GR activities during the experiments but reversed the decrease in CAT activity induced by diuron at 72 h. Pretreatment with putrescine (PUT) prevented the increase in SOD activity induced by diuron at 48 and 72 hrs while it increased GPX and GR activities at 48 h of diuron treatment. Also, the decrease in CAT activity induced by diuron at 72 h was completely prevented by Put. it can be concluded that pretreatment of maize leaves with polyamines reduced the damage produced by diuron and the protective effects of polyamines against diuron toxicity were closely associated with antioxidant system.

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“…The activity of peroxidases was enhanced in tobacco transformed by the ipt gene (Schnablová et al. , 2006), and an enhancement of peroxidase activity after the exogenous application of benzyladenine to maize leaves has also been described (Durmus and Kadioglu, 2005). The results described in this paper, together with previously published data, indicate that enhanced cytokinin content in plant tissues raises the levels of peroxidases and laccases, the synthesis of electron acceptor precursor and the level of cytokinin dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones

et al. 2010

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SUMMARYHydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one (DIMBOA) was isolated from maize phloem sap as a compound enhancing the degradation of isopentenyl adenine by maize cytokinin dehydrogenase (CKX), after oxidative conversion by either laccase or peroxidase. Laccase and peroxidase catalyze oxidative cleavage of DIMBOA to 4-nitrosoresorcinol-1-monomethyl ether (coniferron), which serves as a weak electron acceptor of CKX. The oxidation of DIMBOA and coniferron generates transitional free radicals that are used by CKX as effective electron acceptors. The function of free radicals in the CKX-catalyzed reaction was also verified with a stable free radical of 2,2¢-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Application of exogenous cytokinin to maize seedlings resulted in an enhanced benzoxazinoid content in maize phloem sap. The results indicate a new function for DIMBOA in the metabolism of the cytokinin group of plant hormones.

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Reduction of paraquat toxicity in maize leaves by benzyladenine

Cited by 16 publications

References 25 publications

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines

Pretreatment with polyamines alleviate the deleterious effects of diuron in maize leaves

Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones

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