Reduction of nitric oxide level results in maturation promoting factor destabilization during spontaneous meiotic exit from diplotene arrest in rat cumulus oocytes complexes cultured <i>in vitro</i>

Tiwari, Meenakshi; Chaube, Shail K.

doi:10.1111/dgd.12390

Cited by 9 publications

(7 citation statements)

References 57 publications

(83 reference statements)

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“…Similar levels of cAMP were also detected in the group of COCs treated with 1.0 mM L-NAME in subsequent hours. These alterations in nucleotide concentrations are different from those observed in rodents (Tiwari and Chaube, 2017). However, we and others (Schwarz et al, 2014;Botigelli et al, 2017;Dubeibe et al, 2017) have also observed the lack of correlation between cGMP and cAMP concentration during meiosis resumption in bovines.…”

Section: Nos Inhibition In Bovine Ivmcontrasting

confidence: 72%

“…NO stimulates the production of cGMP via activation of the soluble guanylate cyclase enzyme (Denninger and Marletta, 1999). Therefore, the reduction in NO production associated with the addition of L-NAME may have induced low cGMP synthesis and may represent one of the possible pathways for meiotic resumption and progression as described in rodents (Pandey et al, 2010;Tiwari and Chaube, 2017).…”

Section: Nos Inhibition In Bovine Ivmmentioning

confidence: 99%

“…This would justify the fact that we did not observe a temporal relationship between the two nucleotides in the resumption of meiosis. More studies are needed to confirm the reproducibility of this model in bovines, as described in rodents (Pandey et al, 2010;Tiwari and Chaube, 2017) to better understand the role of these nucleotides in the two compartments, somatic and germinal, during bovine nuclear maturation in vitro.…”

Section: Nos Inhibition In Bovine Ivmmentioning

confidence: 99%

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Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Marin

Caldas-Bussiere

Maciel

et al. 2020

Zygote

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Summary This study aimed to assess the effects of the inhibition of nitric oxide synthase (NOS) on events that modulate bovine in vitro oocyte maturation. Cumulus–oocyte complexes (COCs) were cultured with hemisections (HSs) of the follicular walls in a maturation medium supplemented with different concentrations (0.1–10.0 mM) of Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME). Controls consisted of COCs cultured in the presence (+HSs) or absence of HSs (–HSs) with no additional l-NAME supplementation. The following parameters were assessed: oocyte nuclear maturation stage; cumulus cell (CC) membrane integrity; nitrate/nitrite, progesterone, and estradiol concentrations in the culture medium at 22 h of cultivation; and the concentrations of cGMP and cAMP in COCs during the first hour of maturation. The addition of 1.0 mM l-NAME increased the percentage of oocytes that reached metaphase II (MII) and the percentage of intact CCs (P < 0.05). All l-NAME concentrations reduced the nitrate/nitrite concentrations (P < 0.05), but none affected steroid concentrations compared with control +HSs (P > 0.05). The addition of 1.0 mM l-NAME reduced cGMP concentrations at 3 h and increased cAMP concentrations in the first hour of culture (P < 0.05). Our findings suggest that the NOS/NO/cGMP pathway participates in meiosis progression (MI to MII) of the bovine oocytes matured in vitro in the presence of hemisections of the follicular walls. Lastly, the mechanisms that lead to the progression of meiosis after NOS inhibition do not involve changes in steroid production.

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Section: Nos Inhibition In Bovine Ivmcontrasting

confidence: 72%

Section: Nos Inhibition In Bovine Ivmmentioning

confidence: 99%

Section: Nos Inhibition In Bovine Ivmmentioning

confidence: 99%

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Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Marin

Caldas-Bussiere

Maciel

et al. 2020

Zygote

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“… 4 , 28–30 Studies from our laboratory using NO donor and iNOS inhibitor suggest that the reduction in NO level results in spontaneous EDA in rat COCs cultured in vitro. 49 Our results suggest that the increase of total ROS level (almost two fold increase) was associated with meiotic exit from diplotene, as well as M-II arrest. The increase of ROS, decrease of iNOS, and thereby NO levels after 7 h of hCG surge could be due to increased cell proliferation and cellular metabolism within the follicle.…”

Section: Discussionmentioning

confidence: 54%

Human Chorionic Gonadotropin Mediated Generation of Reactive Oxygen Species Is Sufficient to Induce Meiotic Exit but Not Apoptosis in Rat Oocytes

Tiwari

Chaube

2017

BioResearch Open Access

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Generation of reactive oxygen species (ROS) is associated with final stages of follicular development and ovulation in mammals. The human chorionic gonadotropin (hCG) mimics the action of luteinizing hormone and triggers follicular development and ovulation. However, it remains unclear whether hCG induces generation of ROS, if yes, whether hCG-mediated increased level of ROS could induce meiotic exit and/or apoptosis in rat oocytes. For this purpose, cumulus–oocyte complexes (COCs) were collected from ovary of experimental rats injected with 20 IU pregnant mare's serum gonadotropin for 48 h followed by 20 IU hCG for 0, 7, 14, and 21 h. The morphological changes in COCs, meiotic status of oocyte, total ROS, hydrogen peroxide (H2O2), inducible nitric oxide synthase (iNOS), nitric oxide (NO), Bax, Bcl-2, cytochrome c, telomerase reverse transcriptase (TERT) expression levels, and DNA fragmentation were analyzed in COCs. Our data suggest that hCG surge increased total ROS as well as H2O2 levels but decreased iNOS expression and total NO level in oocytes. The hCG-mediated increased level of ROS was sufficient to induce meiotic cell cycle resumption in majority of oocytes as evidenced by meiotic exit from diplotene as well as metaphase-II (M-II) arrest and their meiotic status. However, increase of ROS level due to hCG surge was not sufficient to trigger Bax and cytochrome c expression levels and DNA fragmentation in COCs. In addition, increased TERT activity was observed in oocytes collected 21 h post-hCG surge showing onset of oocyte aging. Taken together, these results suggest that hCG induces generation of ROS sufficient to trigger meiotic exit from diplotene, as well as M-II arrest, but not good enough to induce apoptosis in rat oocytes.

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“…cGMP is produced by the granulosa cells and transported into the oocyte via gap junctions and plays an important role in maintaining meiotic arrest of oocytes 49 . Nitric oxide also inhibits germinal vesicle breakdown (GVBD) in oocytes of preovulatory follicles 50 . Germinal vesicle breakdown (GVB) is the dissolution of the nucleus of an oocyte that is arrested in the prophase of meiosis I, a process that enables the oocyte to resume meiosis.…”

Section: Seeds Ofmentioning

confidence: 99%

Untitled

2021

IJPSR

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Aframomum melegueta (Alligator pepper) is a tropical plant that belongs to the Zingiberaceae (Ginger) family, consumed for its culinary and health benefits. This study investigated the therapeutic efficacy of ethanolic seed extract of Aframomum melegueta at ameliorating paraquat-induced ovarian toxicity in rat models. Thirty female Wistar rats were divided into control and five test groups (n=5). Group 1 (Control) received feed and water, while other groups (2-6) were treated orally and daily for 4 weeks as follows; group 2 (400 mg/kg b.w. of Aframomum meleguate seed extract); group 3 (200 mg/kg b.w. of Aframomum meleguate seed extract); group 4 (20 mg/kg b.w. of paraquat); group 5 (co-administration of 20 mg/kg b.w. of paraquat and 400 mg/kg b.w. of Aframomum meleguate seed extract); group 6 (co-administration of 20 mg/kg b.w. of paraquat and 200 mg/kg b.w. of Aframomum meleguate seed extract). At the end of the experiment, oxidative stress markers (malondialdehyde (MDA) and superoxide dismutase (SOD)), estradiol levels, and histology of the ovaries were assessed. Data obtained were analyzed using SPSS version 23 software. Oral gavage of paraquat caused ovarian degeneration, development of ovarian cysts, reduced sera estradiol levels, and higher MDA levels, while SOD levels were reduced (p<0.05) when compared to Control. Co-administration of paraquat with ethanolic seed extract of Aframomum melegueta however, improved all these parameters (ovarian histoarchitecture, MDA, SOD, and sera estradiol levels). Aframomum melegueta thus has a strong antioxidant capability, acting in a dose-dependent manner to ameliorate ovarian toxicity induced by paraquat in Wistar rat models. INTRODUCTION:Background of Study: Paraquat (1, 1'-Dimethyl-4, 4'-bipyridinium dichloride) is a potent, rapidly absorbed herbicide 1 that destroys plant tissue by disrupting photosynthesis and rupturing the cell

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Reduction of nitric oxide level results in maturation promoting factor destabilization during spontaneous meiotic exit from diplotene arrest in rat cumulus oocytes complexes cultured in vitro

Cited by 9 publications

References 57 publications

Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Partial inhibition of nitric oxide synthase activity stimulates the nuclear maturation progression of bovine cumulus–oocyte complex in vitro in the presence of hemisections of the follicular walls

Human Chorionic Gonadotropin Mediated Generation of Reactive Oxygen Species Is Sufficient to Induce Meiotic Exit but Not Apoptosis in Rat Oocytes

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