Reduction of nitrate prior to kjeldahl digestion

Pruden, G.; Kalembasa, S.; Jenkinson, D. S.

doi:10.1002/jsfa.2740360203

Cited by 53 publications

(16 citation statements)

References 7 publications

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“…Soil was fumigated and extracted with 0.5 M K 2 SO 4 in the same manner as for microbial-biomass-C estimation. Total N in the K 2 SO 4 extracts from fumigated and unfumigated soils was determined according to Pruden et al (1985). Microbial-biomass N (MBN) was calculated using the equation: MBN = 2.22 EN where EN is total N extracted from fumigated soil minus total N extracted from unfumigated soil.…”

Section: Measurement Of Microbial Biomassmentioning

confidence: 99%

Impact of organic manures with and without mineral fertilizers on soil chemical and biological properties under tropical conditions

Kaur

Kapoor²,

Gupta

2005

Z. Pflanzenernähr. Bodenk.

195

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Soils receiving organic manures with and without chemical fertilizers for the last 7 yr with pearlmillet–wheat cropping sequence were compared for soil chemical and biological properties. The application of farmyard manure, poultry manure, and sugarcane filter cake alone or in combination with chemical fertilizers improved the soil organic C, total N, P, and K status. The increase in soil microbial‐biomass C and N was observed in soils receiving organic manures only or with the combined application of organic manures and chemical fertilizers compared to soils receiving chemical fertilizers only. Basal and glucose‐induced respiration, potentially mineralizable N, and arginine ammonification were higher in soils amended with organic manures with or without chemical fertilizers, indicating that more active microflora is associated with organic and integrated system using organic manures and chemical fertilizers together which is important for nutrient cycling.

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Section: Measurement Of Microbial Biomassmentioning

confidence: 99%

Impact of organic manures with and without mineral fertilizers on soil chemical and biological properties under tropical conditions

Kaur

Kapoor²,

Gupta

2005

Z. Pflanzenernähr. Bodenk.

195

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“…Bolting stem N content was determined titrimetrically using the Kjeldahl procedure with salicylic acid, sodium thiosulfate, and zinc as catalysts (Pruden et al, 1985). For measuring the S content, 0.1 g aliquot of the ground materials was digested with HNO 3 and HClO 4 .…”

Section: Analyses Of Bolting Stem Carbon Nitrogen and Sulfur Contentsmentioning

confidence: 99%

Effect of CO2 enrichment on the glucosinolate contents under different nitrogen levels in bolting stem of Chinese kale (Brassica alboglabra L.)

Fang

Teng

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:The effects of CO 2 enrichment on the growth and glucosinolate (GS) concentrations in the bolting stem of Chinese kale (Brassica alboglabra L.) treated with three nitrogen (N) concentrations (5, 10, and 20 mmol/L) were investigated. Height, stem thickness, and dry weights of the total aerial parts, bolting stems, and roots, as well as the root to shoot ratio, significantly increased as CO 2 concentration was elevated from 350 to 800 μl/L at each N concentration. In the edible part of the bolting stem, 11 individual GSs were identified, including 7 aliphatic and 4 indolyl GSs. GS concentration was affected by the elevated CO 2 concentration, N concentration, and CO 2 ×N interaction. At 5 and 10 mmol N/L, the concentrations of aliphatic GSs and total GSs significantly increased, whereas those of indolyl GSs were not affected, by elevated atmospheric CO 2 . However, at 20 mmol N/L, elevated CO 2 had no significant effects on the concentrations of total GSs and total indolyl GSs, but the concentrations of total aliphatic GSs significantly increased. Moreover, the bolting stem carbon (C) content increased, whereas the N and sulfur (S) contents decreased under elevated CO 2 concentration in the three N treatments, resulting in changes in the C/N and N/S ratios. Also the C/N ratio is not a reliable predictor of change of GS concentration, while the changes in N and S contents and the N/S ratio at the elevated CO 2 concentration may influence the GS concentration in Chinese kale bolting stems. The results demonstrate that high nitrogen supply is beneficial for the growth of Chinese kale, but not for the GS concentration in bolting stems, under elevated CO 2 condition.

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“…The remaining roots were collected by direct visual examination of subsamples of the bulked and well-mixed lysimeter organic material using a wet sieving technique and a 2-mm sieve. The vegetation was dried at 70 "C and ground to be analysed for total N with prior reduction of nitrate (Pruden et al 1985). The organic material in the lysimeters was weighed and samples were taken for KC1 extractions pre-and post-chloroform fumigation and dry mass determinations.…”

Section: Combinationsmentioning

confidence: 99%

“…The dried and ground material was digested after reducing oxidised nitrogen compounds using the method of Pruden et al (1985). Aliquots of 0.4 g of material were weighed into Kjeldahl flaks and 300 mg Zn powder placed on top of each sample.…”

Section: Total Nitrogen Determinations In Plant and Organic Horizonsmentioning

confidence: 99%

The effect of harvesting intensity on the fate of applied 15N-ammonium to the organic horizons of a coniferous forest in N. Wales

Emmett¹,

Quarmby

1991

Biogeochemistry

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Abstract. '5N-ammonium sulphate equivalent to 0.5 kg N/ha was added as a tracer to lysimeters containing the organic horizons of an acid forest soil. The effect of logging debris (brash), vegetation and second rotation Picea sitchensis seedlings on the amount of the 15N found in various soil, vegetation and leachate pools was followed over a period of 60 days. Transformation of '5N-ammonium to nitrate occurred within 24 hours. Although total nitrate leachate losses were high, tracer-derived nitrate represented only 0.4%-4.2% of the applied '"N-ammonium.The atom % excess of the KCI-extractable organic-N pool was initially lower than for the inorganic species but due to the large pool size, consistently represented 3-6% of the applied '"N-ammonium. The similarity of the atom % excess of the ammonium and nitrate pools indicated an autotrophic nitrification pathway.

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Reduction of nitrate prior to kjeldahl digestion

Cited by 53 publications

References 7 publications

Impact of organic manures with and without mineral fertilizers on soil chemical and biological properties under tropical conditions

Impact of organic manures with and without mineral fertilizers on soil chemical and biological properties under tropical conditions

Effect of CO2 enrichment on the glucosinolate contents under different nitrogen levels in bolting stem of Chinese kale (Brassica alboglabra L.)

The effect of harvesting intensity on the fate of applied 15N-ammonium to the organic horizons of a coniferous forest in N. Wales

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