Reduction of Newcastle Disease Vaccine Dose Using a Novel Adjuvant for Cellular Immune Response in Poultry

Arous, Juliette Ben; Deville, S.; Pal, J. K.; Baksi, Surajit; Boyer, François; Dupuis, Laurent

doi:10.1016/j.provac.2013.06.006

Cited by 15 publications

(11 citation statements)

References 5 publications

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“…In general, one of the important roles of adjuvants is to reduce the necessary amount of antigen in each vaccine dose (Luke and Subbarao 2006). Vogel and Powell had analyzed more than 100 adjuvants in the year 1995 (Arous et al 2013). Because most of these adjuvants are hard to produce, expensive, and have poisonous effects, their usage is limited, which were explained in the year 2000 by Stewart-Tull (Atmar and Keitel 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Alum is one of the first and oldest adjuvants, which is used in producing vaccines for humans and is approved by the Food and Drug Administration (FDA) (Arous et al 2013, Arthanari et al 2014, Luke and Subbarao 2006, Vasievich and Huang 2011. In the year 2000, Gupta and Rost reported that aluminum compounds, especially aluminum phosphate and alum, were used as adjuvants in the production of human vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…Influenza (AI) and Newcastle (ND) diseases are known to have a significant economic effect on the poultry industry (Arous et al 2013, Brugh et al 1979. Type A Influenza, subtype H9N2, belongs to the enveloped viruses of Orthomyxoviridae family with negative single-stranded and segmented RNA, which usually causes respiratory infections, decrease in egg production, and death in poultry (Lee et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Comparative assessment of humoral immune responses of aluminum hydroxide and oil-emulsion adjuvants in Influenza (H9N2) and Newcastle inactive vaccines to chickens

Jafari

Pour

Taghizadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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Context Adjuvants are compounds used in the preparation of inactive vaccines to enhance the immune response. Aluminum hydroxide (alum) is one of the first compounds approved by the Food and Drug Administration, which is used as adjuvants in vaccine products for humans. Montanide ISA 70 is an oil-emulsion adjuvant and is used in poultry inactive vaccines. Objective In this study, the effects of alum adjuvant on the efficiency and induction of immune response in inactive vaccines of Influenza and Newcastle are compared with those of ISA 70. Materials and methods Six groups of 7-d-old specific-pathogen-free chickens were inoculated with 0.3 ml of the prepared vaccines via the subcutaneous route in the neck. Immune response in each group after 7, 14, 21, 31, 41, and 45 d was evaluated using the technique of hemagglutination inhibition. Results The results were compared using SPSS software. Results showed that vaccines containing adjuvant ISA 70 depicted a higher increase in the immune response and adjuvant of 20% alum is similar to adjuvant of ISA 70 in boosting the immune system. There was no statistically significant difference between 10% and 20% alum, but these adjuvants are visibly different from ISA 70. Conclusion In conclusion, alum can be used as an easily accessible, harmless, and effective adjuvant; however, to increase the immune period using the inactive vaccines for poultry, more research would be necessary.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative assessment of humoral immune responses of aluminum hydroxide and oil-emulsion adjuvants in Influenza (H9N2) and Newcastle inactive vaccines to chickens

Jafari

Pour

Taghizadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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“…The use of LPS to reduce the vaccine dosage as well to incorporate the cellular immunity proved out to be a dual advantage. The results obtained by our study were favored by a similar study which used Monatanide™ for cellular immune response in poultry and also emphasized on its capability to increase the cellular immune response that could help to extend the cross-protection against different viral strains or serotypes with reduction of injection doses of inactivated ND poultry vaccines [ 13 ].…”

Section: Discussionmentioning

confidence: 86%

Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken

et al. 2018

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Background and AimNewcastle disease (ND) is considered one of the most important poultry diseases with chicken morbidity and mortality rates up to 100%. Current vaccination programs allow the use of live attenuated vaccines in the field to protect against the disease, which alone is inefficient and requires repeat booster doses. Toll-like receptor agonists (e.g., lipopolysaccharide [LPS]) as adjuvants are the ones, most extensively studied and have shown to be very promising in delivering a robust balanced immune response. In the present study, we have evaluated the potential of LPS to elicit a strong immune response with respect to the elicitation of both Th1 (cell-mediated) and Th2 (humoral) immune arms.Materials and MethodsA total of 72 apparently healthy 1-day-old indigenous unvaccinated chicks were randomly divided into six experimental Groups A to F (n=12). At 8-week of age chicks in Group A, C, and E were vaccinated with live attenuated La Sota strain ND vaccine along with LPS, bovine serum albumin, and normal saline solution, respectively, and those in Group B, D, and E were kept separately without vaccination. Sampling was done on days 0, 1, 3, 7, 14, 21, 35, and 60 after vaccination. After vaccination and respective adjuvant application, Th1 and Th2 cytokine expression were measured in mRNA of both blood and tissue samples.ResultsThe results were validated by, hemagglutination inhibition and enzyme-linked immunosorbent assay tests, to check for the humoral as well as cell-mediated immune response in blood serum levels. The results showed an increase in mRNA expression of the Th1 biased cytokines in Group A (LPS+NDV) as compared to the control groups. Similar mRNA expression pattern was seen in blood as well as tissue samples. Validation of results also indicates an increase in Cell-mediated Immunity as well as a humoral immune response in Group A (LPS+NDV).ConclusionThe results of the study provided enough evidence to consider LPS as a potential vaccine adjuvants candidate against ND in chicken.

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“…To improve the immunization, inactivated NDV vaccine is usually supplemented with adjuvants, and the vaccine is usually made from emulsification of an aqueous phase containing antigen with an adjuvant oil phase. Generally, the adjuvant used for NDV vaccine originates from the mineral oil ( Ben Arous et al., 2013 ; Lone et al., 2017 ). In China, immunization of NDV vaccine is compulsory for fowl ( Yu et al., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Sunflower seed oil containing ginseng stem–leaf saponins (E515-D) is a safe adjuvant for Newcastle disease vaccine

Wang

et al. 2020

Poultry Science

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Vaccination is an effective method to prevent Newcastle disease ( ND ) in chickens. Marcol 52 and #10 white oil are mineral-based adjuvants and can be found in commercial inactivated ND virus vaccines. The present study demonstrated that a vegetable origin oil E515-D had lower polycyclic aromatic hydrocarbons and higher flash point than the commercial products Marcol 52 and #10 white oil. E515-D could be mixed with an aqueous phase containing ND virus antigen to form a stable water-in-oil vaccine emulsion and exhibited more potent adjuvant effects on the immune response than Marcol 52 and #10 white oil. Moreover, the absorption of E515-D–adjuvanted vaccine was faster than absorption of Marcol 52- and #10 white oil-adjuvanted vaccines when ND virus vaccines were injected in broilers. Therefore, E515-D was safe and could be a suitable adjuvant used in vaccines for food animals. In addition，E515-D is not easy to be flammable during shipping and storage owing to its higher flash point.

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Reduction of Newcastle Disease Vaccine Dose Using a Novel Adjuvant for Cellular Immune Response in Poultry

Cited by 15 publications

References 5 publications

Comparative assessment of humoral immune responses of aluminum hydroxide and oil-emulsion adjuvants in Influenza (H9N2) and Newcastle inactive vaccines to chickens

Comparative assessment of humoral immune responses of aluminum hydroxide and oil-emulsion adjuvants in Influenza (H9N2) and Newcastle inactive vaccines to chickens

Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken

Sunflower seed oil containing ginseng stem–leaf saponins (E515-D) is a safe adjuvant for Newcastle disease vaccine

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