Reduction of molybdate to molybdenum blue by <i>Klebsiella</i> sp. strain hkeem

Lim, Hyun‐Tae; Syed, Mohd Arif; Shukor, Mohd Yunus

doi:10.1002/jobm.201100121

Cited by 41 publications

(103 citation statements)

References 30 publications

(21 reference statements)

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“…Therefore, K. oxytoca strain Saw-5 is a potential candidate for soil bioremediation of sites containing elevated levels of molybdenum either locally or in other tropical countries. The optimum temperature observed in this work falls within the optimal temperature range reported in the majority of the molybdenum reducers isolated to date, which exhibit optimal temperature of between 25 and 37ºC (Shukor and Syed, 2010;Lim et al, 2012;AboShakeer et al, 2013;Othman et al, 2013;Halmi et al, 2013;Khan et al, 2014; with the only psychrotolerant reducer isolated from Antartica showing an optimal temperature supporting reduction of between 15 and 20 ºC .…”

Section: Mo-blue Production As a Function Of Ph And Temperaturesupporting

confidence: 75%

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ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

et al. 2016

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Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 o C. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.

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Section: Mo-blue Production As a Function Of Ph And Temperaturesupporting

confidence: 75%

“…However, for now the bacterium is tentatively identified as K. oxytoca strain Saw-5. Previously, two molybdenumreducing bacterium from this genus; K. oxytoca strain Dr.Y14 and K. oxytoca strain hkeem (Lim et al, 2012) have been isolated.…”

Section: Identification Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

et al. 2016

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“…High phosphate concentrations inhibit the stability of phosphomolybdate, of which upon reduction, it is converted to Mo-blue Glenn & Crane 1956;Sims 1961;Shukor et al 2000). Molybdenum-reducing bacteria isolated previously are also strongly inhibited by phosphate concentration higher than 5 mM Lim et al 2012;Ahmad et al 2013;Halmi et al 2013;Othman et al 2013;AboShakeer et al 2013;Khan et al 2014;.…”

Section: Effect Of Phosphate and Molybdate Concentrations To Molybdatmentioning

confidence: 91%

“…Molybdate reduction to Mo-blue in many of the previously isolated Molybdenum-reducing bacteria were inhibited by similar toxic heavy metals Lim et al 2012;Othman et al 2013;. In hexavalent chromate reduction to the trivalent state by Bacillus sp.…”

Section: Effect Of Heavy Metalsmentioning

confidence: 96%

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ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND PHENOLIC- AND CATECHOL-DEGRADING Enterobacter sp. STRAIN SAW-2

Sabullah

Rahman²,

Ahmad³

et al. 2017

BIOTROPIA

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Molybdenum is an emerging pollutant worldwide. The objective of this study is to isolate molybdenum-reducing bacterium with the ability to grow on phenolic compounds (phenol and catechol). The screening process was carried out on a microplate. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). The bacterium required a narrow pH range for optimal reduction of molybdenum, i.e. between pH 6.3 and o 6.8, with temperature between 34 and 37 C. Molybdate reduction to Mo-blue was best supported by glucose as the carbon source. However, both phenol and catechol could not support molybdate reduction. Other requirements for molybdate reduction included sodium molybdate concentrations between 15 and 30 mM, and phosphate concentration of 5.0 mM. The bacterium exhibited a Mo-blue absorption spectrum with a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. strain Saw-2. The capability of this bacterium to grow on toxic phenolic compounds and to detoxify molybdenum made it a significant agent for bioremediation.

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Production of allitol from D‐psicose by a novel isolated strain of Klebsiella oxytoca G4A4

Han

Zhu

Men

et al. 2014

J. Basic Microbiol.

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A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 °C with a density of 40 (OD600 nm ). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy.

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Reduction of molybdate to molybdenum blue by Klebsiella sp. strain hkeem

Cited by 41 publications

References 30 publications

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND PHENOLIC- AND CATECHOL-DEGRADING Enterobacter sp. STRAIN SAW-2

Production of allitol from D‐psicose by a novel isolated strain of Klebsiella oxytoca G4A4

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