Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

Karaöz, Erdal; Kabataş, Serdar; Duruksu, Gökhan; Okçu, Alparslan; Subaşı, Cansu; Ay, Birol; Müslüman, Murat; Çivelek, Erdinç

doi:10.5137/1019-5149.jtn.5412-11.1

Cited by 26 publications

(29 citation statements)

References 19 publications

(17 reference statements)

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“…The MSCs were transfected by Neon transfection System (Invitrogen/ Life Sciences) according to the method described previously [13]. The stable cell lines were maintained by continuous culture of transfected cells in the culture media with G418 (Gibco/Life Sciences; 200 μg/mL).…”

Section: Green Fluorescent Protein (Gfp) Labeling Of Stem Cellsmentioning

confidence: 99%

Cross Effects of Resveratrol and Mesenchymal Stem Cells on Liver Regeneration and Homing in Partially Hepatectomized Rats

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Şimşek

Subaşı

et al. 2014

Stem Cell Rev and Rep

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In this study, we examined the effect of preoperatively administered resveratrol (RV) and mesenchymal stem cells (MSCs) on regeneration of partially hepatectomized rat liver. We also evaluated the effect of RV on homing of MSCs. MSCs were isolated from bone marrow and cultured in vitro. Wistar albino rats were randomly divided into four groups. In groups, rats received (1) no treatment, (2) single dose RV, (3) MSCs and (4) RV plus MSCs before partial hepatectomy (PH). Injected MSCs were traced by labeling them with green fluorescent protein, and liver regeneration was determined by comparison of liver weight gain, histological examination and immunohistochemical staining with proliferating cell nuclear antigen (PCNA) for mitotic cells. The expression levels of tumor necrosis factor -alpha (TNF-α), interleukin-6 (IL-6) and hepatocyte growth factor (HGF) were also determined in the parafin sections of liver specimens with immunohistochemical staining. Administration of RV and MSCs separately or together enhanced liver regeneration despite decreasing the TNF-α and IL-6 expression. This positive contribution was probably due to direct raising effect on HGF for RV and HGF expression for MSCs that we demonstrated with immunohistochemical staining. Additionally, RV increased the homing of MSCs in liver probably related to life prolonging effect on MSCs. These results indicate that preoperative RV as well as MSCs application enhances liver regeneration after partial hepatectomy in rats. Paying attention to RV about the effect on liver regeneration and homing of MSCs might be the goal of further investigations.

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Section: Green Fluorescent Protein (Gfp) Labeling Of Stem Cellsmentioning

confidence: 99%

Cross Effects of Resveratrol and Mesenchymal Stem Cells on Liver Regeneration and Homing in Partially Hepatectomized Rats

Okay

Şimşek

Subaşı

et al. 2014

Stem Cell Rev and Rep

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“…During the acute phase, neuronal necrosis, axonal disruption, blood supply insufficiency, edema and excitotoxic neurotransmitters are triggered. These changes continue into the subacute phase, during which ischemia, oxidative stress, lipid peroxidation and inflammation predominate (9,15,17,25). In this study, the effects of transplanting rAT-MSCs immediately (acute) and 9 days (subacute) p.i.…”

Section: Groupsmentioning

confidence: 99%

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

Aras¹,

Sabancı²,

Kabataş³

et al. 2015

Turkish Neurosurgery

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AIM:To investigate the effectiveness of rat adipose tissue-derived (rAT) mesenchymal stem cell (MSC) transplantation on the functional restoration and regeneration of spinal cord injury (SCI). MATERIAl and METhODS:Six of 48 Wistar albino rats were sacrificed to obtain MSCs, and the remaining rats were divided randomly into six groups. SCI was performed using the clip compression method. The control and transplantation groups were injected with physiological saline and a rAT-MSC suspension at the injury sites, respectively. Each animal was evaluated using the Basso, Beattie and Bresnahan (BBB) rating system and sacrificed at 28 days post-injury period (p.i.). The regeneration process was evaluated based on immunostaining against β3-tubulin, BDNF, CNTF, and CNPase.RESulTS: rAT-MSC transplantation into the SCI site substantially improved the tissue regeneration and functional recovery (p < 0.05). However, the rAT-MSC transplantation at 9 days p.i. was not more efficient on functional recovery than the transplantation immediately after injury. The expression of β3-tubulin, BDNF and CNTF at the injury site indicated the potential for functional regeneration. COnCluSIOn:The adaptive nature of rat-MSCs enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of SCI.

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“…In order to determine the multipotency of isolated bone marrow mesenchymal stem cells, rBMSCs were harvested in the third passage and divided into three groups: adipogenic, osteogenic and neurogenic differentiation protocols were performed as described previously [26]. DMEM supplemented with 10% FBS, 1% Penicillin/Streptomycin were added to each group and incubated for 2 days for confluency.…”

Section: In Vitro Differentiation Of Rbmscsmentioning

confidence: 99%

Effects of Mesenchymal Stromal Cells on the Neuropathic Pain Induced by Chronic Constriction Injury in Rats

Genç¹,

Zibandeh²,

Yıldız³

et al. 2017

J Pain Relief

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Background: Neuropathic pain remains a persistent clinical problem and characterized by mechanical allodynia and heat hyperalgesia. Chronic pain conditions are among the major health problems which are difficult to treat. Bone marrow-derived mesenchymal stromal cells (BMSCs) have generated great interest as an option for cell-based therapy. BMSCs are easy to isolate and expand ex vivo. Clinical studies show that direct injection of BMSCs does not produce side effects as rejection and is well tolerated by the immune system.

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Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

Cited by 26 publications

References 19 publications

Cross Effects of Resveratrol and Mesenchymal Stem Cells on Liver Regeneration and Homing in Partially Hepatectomized Rats

Cross Effects of Resveratrol and Mesenchymal Stem Cells on Liver Regeneration and Homing in Partially Hepatectomized Rats

The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

Effects of Mesenchymal Stromal Cells on the Neuropathic Pain Induced by Chronic Constriction Injury in Rats

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