Reduction of infection risk mediated by co‐culturing <scp><i>Vibrio parahaemolyticus</i></scp> and <scp><i>Listeria monocytogenes</i></scp> in refrigerated cooked shrimp

Niu, Ben; Mu, Lili; Xiao, Lili; Zhang, Zhaohuan; Malakar, Pradeep K.; Liu, Haiquan; Pan, Yingjie; Yong, Zhao

doi:10.1002/jsfa.8969

Cited by 7 publications

(8 citation statements)

References 31 publications

(64 reference statements)

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“…The overnight culture was then diluted with 0.1% peptone water (PW; Beijing Luqiao Technology Co., Ltd., Beijing, China) to an optical density (OD) value for 1.2 ± 0.02 (about 10 9 CFU/ml) at 600 nm (OD 600). The automated turbidimetric system Bioscreen C (Oy Growth Curves Ab Ltd., Raisio, Finland) was used to test the corresponding OD values at regular intervals (Niu et al, 2018b).…”

Section: Bacterial Strains and Preparation Of Inoculummentioning

confidence: 99%

Comparison on the Growth Variability of Vibrio parahaemolyticus Coupled With Strain Sources and Genotypes Analyses in Simulated Gastric Digestion Fluids

Wang

Yong²,

Pan³

et al. 2020

Front. Microbiol.

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Vibrio parahaemolyticus is a food-borne pathogen that causes pathogenic symptoms such as diarrhea and abdominal pain. Currently no studies have shown that either pathogenic and non-pathogenic V. parahaemolyticus possess growth heterogeneity in a human environment, such as in gastric and intestinal fluids. The tlh gene is present in both pathogenic and non-pathogenic V. parahaemolyticus strains, while the tdh and trh genes are only present in pathogenic strains. This study firstly applied simulated human gastric fluids to explore growth variability of 50 strains of V. parahaemolyticus at 37 • C. The bacterial growth curves were fitted by primary modified Gompertz model, and the maximum growth rate (µ max ), lag time (LT), and their CV values were calculated to compare the stress response of pathogenic and non-pathogenic V. parahaemolyticus to simulated human gastric fluids. Results showed that the simulated human gastric fluids treatment significantly increased the µ max of pathogenic strains and shortened the lag time, while decreased the µ max of non-pathogenic strains and prolonged the lag time. Meanwhile, the CV values of genotypes (tlh + /tdh + /trh − ) evidently increased, showing that the pathogenic genotype (tlh + /tdh + /trh − ) strains had strong activity to simulated gastric fluids. All of the results indicated that the V. parahaemolyticus strains exhibited a great stress-resistant variability and growth heterogeneity to the simulated gastric fluids, which provides a novel insight to unlock the efficient control of pathogenic V. parahaemolyticus.

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Section: Bacterial Strains and Preparation Of Inoculummentioning

confidence: 99%

Comparison on the Growth Variability of Vibrio parahaemolyticus Coupled With Strain Sources and Genotypes Analyses in Simulated Gastric Digestion Fluids

Wang

Yong²,

Pan³

et al. 2020

Front. Microbiol.

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“…L. monocytogenes was streaked onto PALCAM (PALCAM; Beijing Land Bridge Technology Company, Ltd., Beijing, China) plates with selective additives and incubated at 37°C for 30 h. Individual colony was grown in 9 mL brain heart infusion (BHI, Beijing Land Bridge Technology Company, Ltd., Beijing, China) and incubated overnight at 37°C with shaking at 180 rpm. The bacterial concentrations were diluted to ∼8 Log CFU mL –1 for use (Niu et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…L. monocytogenes infection may cause listeriosis (de Grandi et al, 2018; Radoshevich and Cossart, 2018) and the mortality rate is high where almost 1/3 infected cases will be fatal (Grace et al, 2011). V. parahaemolyticus and L. monocytogenes can coexist in different seafood products and in post-harvest processing sites and these are potential sources of cross contamination (Liao et al, 2015; Zhang et al, 2015a; Niu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes

et al. 2019

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Mixed-species biofilms are the predominant form of biofilms found in nature. Research on biofilms have typically concentrated on single species biofilms and this study expands the horizon of biofilm research, where the characterization and dynamic changes of mono and mixed-species biofilms formed by the pathogens, Vibrio parahaemolyticus and Listeria monocytogenes were investigated. Compared to mono-species biofilm, the biomass, bio-volume, and thickness of mixed-species biofilms were significantly lower, which were confirmed using crystal violet staining, confocal laser scanning microscopy and scanning electron microscopy. Further experimental analysis showed these variations might result from the reduction of bacterial numbers, the down-regulation of biofilm-regulated genes and loss of metabolic activity in mixed-species biofilm. In addition, V. parahaemolyticus was located primarily on the surface layers of the mixed-species biofilms thus accruing competitive advantage. This competitive advantage was evidenced in a higher V. parahaemolyticus population density in the mixed-species biofilms. The adhesion to surfaces of the mixed-species biofilms were also reduced due to lower concentrations of extracellular polysaccharide and protein when the structure of the mixed-species was examined using Raman spectral analysis, phenol-sulfuric acid method and Lowry method. Furthermore, the minimum biofilm inhibitory concentration to antibiotics obviously decreased when V. parahaemolyticus co-exited with L. monocytogenes. This study firstly elucidated the interactive behavior in biofilm development of two foodborne pathogens, and future studies for biofilm control and antibiotic therapy should take into account interactions in mixed-species biofilms.

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“…Afterward, the density of the bacterial suspensions was measured by BioTek Synergy2 (Winooski, VT, United States) at 600 nm (OD 600 nm ). When the OD 600nm values of V. parahaemolyticus are in the interval (1.2, 1.3), the bacteria concentrations are 9 Log 10 CFU/g ( Niu et al, 2018 ). Dead cells were obtained by heating suspensions at 85°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Rapid detection technique can improve the management of microbial food safety and lots of detection methods were developed for V. parahaemolyticus ( Nordin et al, 2017 ; Wu et al, 2018 ; Yang et al, 2018 ). Standard detection for V. parahaemolyticus , which is time and labor intensive, consists of isolation, purification and identification based on thiosulfate-citrate-bile-salts-sucrose (TCBS) agar and Polymerase Chain Reaction (PCR) ( Niu et al, 2018 ). This method, however, fails to detect viable but non-culturable (VBNC) cells and V. parahaemolyticus has been shown to easily enter VNBC state as a response to adversity ( Coutard et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+, tdh+, and ureR+)

et al. 2018

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Pathogenic and non-pathogenic Vibrio parahaemolyticus strains were simultaneously detected and quantified using a novel viable multiplex real-time PCR (novel qPCR). We used a new PCR primer and probe, ureR, as a surrogate for detection of the toxin trh gene as the primer was better at identifying variant V. parahaemolyticus trh strains. The specificity of all primers and probes used in this study were validated on three standard strains of V. parahaemolyticus, 42 clinical strains, 12 wild strains, 4 strains of Vibrio spp., and 4 strains of other bacteria. Then, propidium monoazide (PMA) was applied to inhibit DNA of dead cell, and the results of PMA optimized treatments were 15 μM concentration, 5 min incubation periods, 15 min light exposure periods and 30 RPM rotational speed, which resulted in time and cost savings. Pathogenic and non-pathogenic strains were quantified using a two-reaction tube method where the tlh, tdh, and ureR genes were amplified. Additionally, standard curves with a 7-log dynamic range were generated for quantifying viable V. parahaemolyticus and the amplification efficiencies were 108.68, 105.17, and 115.61% for tlh+, tdh+, and ureR+. This novel qPCR accurately monitored V. parahaemolyticus contamination rates in shrimps (Penaeus vannamei) and clams (Ruditapes philippinarum) sampled from retail stores located in a major district in Shanghai. In conclusion, our assay can prioritize the detection and quantification of viable pathogenic V. parahaemolyticus and can prove to be a more effective tool for reducing infection risks from consumption of seafood in Shanghai.

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Reduction of infection risk mediated by co‐culturing Vibrio parahaemolyticus and Listeria monocytogenes in refrigerated cooked shrimp

Abstract: This study has revealed that interaction of V. parahaemolyticus and L. monocytogenes should be considered when quantifying risks posed by these pathogens during consumption of seafood products. © 2018 Society of Chemical Industry.

Cited by 7 publications

References 31 publications

Comparison on the Growth Variability of Vibrio parahaemolyticus Coupled With Strain Sources and Genotypes Analyses in Simulated Gastric Digestion Fluids

Comparison on the Growth Variability of Vibrio parahaemolyticus Coupled With Strain Sources and Genotypes Analyses in Simulated Gastric Digestion Fluids

Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes

A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+, tdh+, and ureR+)

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