Reduction of Fusarium wilt symptoms in tomato seedlings following seed treatment with Origanum vulgare L. essential oil and carvacrol

Gonçalves, Débora Cristina; Queiroz, Vagner Tebaldi de; Costa, Adílson Vidal; Lima, Wilker Pinheiro; Belan, Leônidas Leoni; Moraes, Willian Bucker; Iório, Natalia Lopes Pontes; Póvoa, Helvécio Cardoso Corrêa

doi:10.1016/j.cropro.2020.105487

Cited by 19 publications

(9 citation statements)

References 38 publications

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“…Therefore, carvacrol did not adversely affect seedling germination at all tested doses. It is thus fitting that our results agree with those reported by Gonçalves et al, who confirmed the absence of phytotoxicity of carvacrol on tomato seeds [49]. Regarding the preventive effect of carvacrol investigated in the present work, the result obtained showed that in the absence of carvacrol, all fungal strains seriously inhibited seed germination whether individually or combined.…”

Section: Discussionsupporting

confidence: 94%

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

et al. 2021

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Background: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to evaluate the preventive potential of carvacrol on germination of Fusarium oxysporum, Neocosmospora solani, and Microdochium nivale spores as responsible agents for Lolium perenne seeds damping-off disease. Material and methods: Macrodilution method in agar medium, spore germination, spore destruction, and preventive treatment bioassays were used to achieve this goal. Results: The minimum inhibitory concentration (MIC) of carvacrol vs. tested strains existed in the range of 0.25–0.5 mg/mL. Carvacrol used in concentrations ranging from 0.2 to 0.4 mg/mL inhibited the germination of all fungal spores in a dose-dependent manner. Carvacrol showed a very strong sporicidal effect against all studied fungal strains, and this effect was well confirmed by microscopic observations. The percentage of growth inhibition was found to be strictly correlated to carvacrol dose up vs. all strains. Carvacrol increased the emergence of L. perenne seeds when compared to both uninfested and infested seeds. Conclusion: Based on the results obtained, carvacrol fulfills the requirement for being a natural alternative agent to fight Gramineae seedlings’ damping-off caused by fungal species without adverse effects on the plants.

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Section: Discussionsupporting

confidence: 94%

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

et al. 2021

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“…It is desirable that the assays also determine the potential fungicidal effect of the extracts and/or compounds. Many plant extracts have demonstrated potent antifungal activity (with MIC values below 1.0 mg/mL) using in vitro assays (Table 2), but only a few were tested in vivo [130,[222][223][224][225][226][227]. The potent in vitro antifungal activity of Melia azedarach, Combretum erythrophyllum and Quercus acutissima leaf extracts [130] were confirmed in vivo.…”

Section: And Future Perspectivesmentioning

confidence: 99%

Natural Products from Medicinal Plants against Phytopathogenic Fusarium Species: Current Research Endeavours, Challenges and Prospects

2021

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Many Fusarium species are pathogenic, causing crop diseases during crop production and spoilage of agricultural products in both commercial and smallholder farming. Fusarium attack often results into food contamination, yield loss and increases in food insecurity and food prices. Synthetic fungicides have been used as a control strategy for the management of crop diseases caused by Fusarium pathogens. The negative effects associated with application of many synthetic pesticides has necessitated the need to search for alternative control strategies that are affordable and environmentally safe. Research on medicinal plants as control agents for Fusarium pathogens has received attention since plants are readily available and they contain wide variety of secondary metabolites that are biodegradable. The activities of solvent extracts, essential oils and compounds from medicinal plants have been tested against Fusarium phytopathogenic species. A summary of recent information on antifungal activity of plants against Fusarium species is valuable for the development of biopesticides. This paper reviews the antifungal research conducted on medicinal plants against Fusarium pathogens, over a 10-year period, from January 2012 to May 2021. We also highlight the challenges and opportunities of using natural products from medicinal plants in crop protection. Several databases (Science Direct and Web of Science) were used to obtain information on botanical products used to control Fusarium diseases on crops. Keywords search used included natural products, antifungal, Fusarium, crops diseases, phytopathogenic, natural compounds and essential oil.

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“…These effects were not detected in untreated seeds, where callose and ROS production increased during germination and decreased in the absence of the fungus. There are previous results describing effects of coated seed priming on tomato ( Król et al, 2015 ; Gonçalves et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Essential Oils Prime Epigenetic and Metabolomic Changes in Tomato Defense Against Fusarium oxysporum

et al. 2022

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In this work, we studied the direct and indirect plant protection effects of an Artemisia absinthium essential oil (AEO) on tomato seedlings against Fusarium oxysporum sp. oxysporum radicis lycopersici (Fol). AEO exhibited a toxic effect in vitro against Fol. Additionally, tomato seedlings germinated from seeds pretreated with AEO and grown hydroponically were protected against Fol. Plant disease symptoms, including, water and fresh weight loss, tissue necrosis, and chlorosis were less pronounced in AEO-treated seedlings. AEO also contributed to plant defenses by increasing callose deposition and the production of reactive oxygen species (ROS) on seed surfaces without affecting seed germination or plant development. The essential oil seed coating also primed a durable tomato seedling defense against the fungus at later stages of plant development. RNA-seq and metabolomic analysis performed on seedlings after 12 days showed that the AEO treatment on seeds induced transcriptomic and metabolic changes. The metabolomic analysis showed an induction of vanillic acid, coumarin, lycopene, oleamide, and an unknown metabolite of m/z 529 in the presence of Fol. The StNRPD2 gene, the second largest component of RNA polymerases IV and V directly involved in de novo cytosine methylation by RNA-directed DNA methylation (RdDM), was highly induced in the presence of AEO. The host methionine cycle (MTC) controlling trans-methylation reactions, was also altered by AEO through the high induction of S-adenosyl methionine transferases (SAMts). Our results suggest that AEO treatment could induce de novo epigenetic changes in tomato, modulating the speed and extent of its immune response to Fol. The EO-seed coating could be a new strategy to prime durable tomato resistance, compatible with other environmentally friendly biopesticides.

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Reduction of Fusarium wilt symptoms in tomato seedlings following seed treatment with Origanum vulgare L. essential oil and carvacrol

Cited by 19 publications

References 38 publications

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

Natural Products from Medicinal Plants against Phytopathogenic Fusarium Species: Current Research Endeavours, Challenges and Prospects

Essential Oils Prime Epigenetic and Metabolomic Changes in Tomato Defense Against Fusarium oxysporum

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