Reduction of expression of IL‐18, IL‐1β genes in the articular joint by sumac fruit extract (Rhus coriaria L.)

Abstract

Background Osteoarthritis is the most common malignant disease in the world. The disease is caused by changes in the metabolism, the structure and function of multiple joints, and joint tissues. Sumac is one of the indigenous plants of Iran and has traditionally been used as a spice in Iran. The aim of this study was to investigate the reduction of expression of IL‐18, IL‐1β genes in the articular joint by sumac fruit extract (Rhus coriaria L.). Methods The alcoholic extract of sumac fruit (E.E.R.C.L) was prepared from the Genetic Reserve Center. Bleeding was used to provide synoviocyte cells from the joint and fluid of the anatomical metacarpal limb of the 8‐month‐old Holstein healthy calf without any signs of inflammation. Using cell‐hemocytometer count, their viability was evaluated by trypan blue and after lipopolysaccharide (LPS) proliferation and injection to enhance the level of cytokines. After isolating the RNA and preparing the cDNA, RT‐PCR and PCR were performed and then, using the real‐time PCR method, the expression of the desired genes was investigated. Results In this study, after the expression of IL‐18 cytokines, IL‐1β increased to 100%, and following the treatment with alcoholic extract, the reduction of expression of these cytokines was 33.61% and 29.01%, respectively. The results of anti‐inflammatory effects showed that the alcoholic extract of sumac reduced the IL‐1β, IL‐18 expression in LPS‐stimulated cells. Conclusion Sumac fruit extract can be an effective medication for reducing pain.

Pharmacological and Antioxidant Activities of Rhus coriaria L. (Sumac)

“…Momeni et al were among the first to show a potential anti-inflammatory activity of Rhus coriaria . The authors demonstrated that sumac fruit alcoholic extract significantly reduced the level of mRNA of the pro-inflammatory cytokines IL-18 and IL-1β, in lipopolysaccharide-stimulated synoviocyte extracted from joint and fluid of limb of the 8-month-old healthy calf [ 122 ]. In another study, Gabr et al .…”

Phytochemical Diversity and Pharmacological Properties of Rhus coriaria

“…Momeni et al. (2019) have reported that R. coriaria fruit extract reduced the expression of IL‐18, IL‐1 genes in the articular joint in healthy calf, using the real‐time PCR method. Results indicates that after the expression of IL‐18 cytokines, IL‐1 β increased to 100 %, and following the treatment with the alcoholic extract, the reduction of expression of these cytokines was 33.61 % and 29.01 %, respectively.…”

Evaluation of Anti-inflammatory Activity of Methanol Extract of Rhus coriaria L. in Diabetic Rats

“…It has been reported that alcoholic extract of R. coriaria fruit significantly reduced the level of mRNA of the pro-inflammatory cytokines IL-18 and IL-1β, in lipopolysaccharide-stimulated synoviocytes extracted from joint and fluid of limb of the 8-month-old healthy calf. 49 Previous studies showed similar results in terms of its anti-inflammatory activity using the carrageenan-induced foot oedema model. An example is the use of Rhus toxicodendron and the methanol extract of Rhus tripartita, which showed significant inhibition of inflammation in the carrageenan-induced foot oedema model.…”