Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI

Hasegawa, Junichi; Yanaihara, Atsushi; Iwasaki, Shinji; Mitsukawa, Kaori; Negishi, Momoko; Okai, Takashi

doi:10.1007/s10815-007-9155-4

Cited by 41 publications

(41 citation statements)

References 29 publications

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“…7) [79].These connexin-43-rich GJC were ruptured or obstructed after 6 h of culture in the bovine (GVBD), and substituted by GJB1 [80]. Consistent with our results which showed a significant decrease of its expression, the GJA1 underexpression was suggested as a good marker of oocyte quality and successful pregnancy [57]. Interestingly, the CCs transzonal projections (TZP) structures based on actin filaments were recently shown to be intact in mouse CCs in vivo [73].…”

Section: Discussionsupporting

confidence: 88%

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Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Assidi

Montag

Ven

et al. 2010

J Assist Reprod Genet

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Purpose To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. Methods Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intrapatient analysis were performed on 17 selected candidates. Results Seven genes were differentially (p≤0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. Conclusions These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection.Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency.

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Section: Discussionsupporting

confidence: 88%

“…We believe that CCs are the site of a two-way dialog between the oocyte and the follicular environment, and could therefore respond by the expression of several useful markers [27,49,57]. Biomarkers of oocyte incompetence could therefore constitute additional and important indicators of oocyte quality.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Assidi

Montag

Ven

et al. 2010

J Assist Reprod Genet

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show abstract

“…[32]. In that study as in ours, Cx43 level was measured in relation to GAPDH in cumulus cells from patients undergoing IVF by ICSI.…”

Section: Discussionmentioning

confidence: 81%

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality

Wang

Tong

El-Gehani

et al. 2009

J Cellular Molecular Medi

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Gap junctional coupling among cumulus cells is important for oogenesis since its deficiency in mice leads to impaired folliculogenesis. Multiple connexins (Cx), the subunits of gap junction channels, have been found within ovarian follicles in several species but little is known about the connexins in human follicles. The aim of this study was to determine which connexins contribute to gap junctions in human cumulus cells and to explore the possible relationship between connexin expression and pregnancy outcome from in vitro fertilization (IVF). Cumulus cells were obtained from IVF patients undergoing intra-cytoplasmic sperm injection (ICSI). Connexin expression was examined by RT-PCR and confocal microscopy. Cx43 was quantified by immunoblotting and gap junctional coupling was measured by patch-clamp electrophysiology. All but 5 of 20 connexin mRNAs were detected. Of the connexin proteins detected, Cx43 forms numerous gap junction-like plaques but Cx26, Cx30, Cx30.3, Cx32 and Cx40 appeared to be restricted to the cytoplasm. The strength of gap junctional conductance varied between patients and was significantly and positively correlated with Cx43 level, but neither was correlated with patient age. Interestingly, Cx43 level and intercellular conductance were positively correlated with embryo quality as judged by cleavage rate and morphology, and were significantly higher in patients who became pregnant than in those who did not. Thus, despite the presence of multiple connexins, Cx43 is a major contributor to gap junctions in human cumulus cells and its expression level may influence pregnancy outcome after ICSI.

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“…Hasegava et al (47) measured the mRNA expression of connexin 43 in cumulus cells during IVF-ICSI cycles. They did not find any significant relationship between expression of connexin 43 and fertilization or cleavage rate.…”

Section: Connexin 43mentioning

confidence: 99%

Standard human chorionic gonadotropin versus double trigger for final oocyte maturation results in different granulosa cells gene expressions: a pilot study

Haas

Ophir

Machtinger

et al. 2016

Fertility and Sterility

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Objective: To investigate the messenger RNA (mRNA) expression of reproduction-related genes in granulosa cells (GCs) of patients triggered with hCG compared with patients triggered with GnRH agonist and hCG (double trigger) for final oocyte maturation. Design: Granulosa cells were obtained at the time of oocyte retrieval, and gene expression was analyzed using quantitative real-time polymerase chain reaction. Setting: Referral center. Patient(s): Fifteen women undergoing controlled ovarian hyperstimulation for IVF who received hCG for final follicular maturation and in a subsequent IVF cycle received double trigger. Intervention(s): Granulosa cells collection. Main Outcome Measure(s): The expression of genes related to ovarian hyperstimulation syndrome, gap junction, and epidermal-like growth factor in GCs. Result(s): The mRNA expressions of amphiregulin (2.1 vs.

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Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI

Abstract: These results suggest that full reduction of Cx43 expression on cumulus cells at the time of oocyte collection during ICSI is essential for developmental competence of human oocytes.

Cited by 41 publications

References 29 publications

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality

Standard human chorionic gonadotropin versus double trigger for final oocyte maturation results in different granulosa cells gene expressions: a pilot study

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