Reduction of Clostridium sporogenes spore outgrowth in natural sausage casings using nisin

Wijnker, Joris J.; Weerts, E.A.W.S.; Breukink, Eefjan; Houben, J.H.; Lipman, L.J.A.

doi:10.1016/j.fm.2011.01.009

Cited by 35 publications

(24 citation statements)

References 31 publications

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“…In contrast, the CD27L C-terminal domain reduced the efficacy of the CS74L catalytic domain when tested against C. tyrobutyricum. The addition of the CD27L C-terminal domain did not enable the lysin to target C. difficile or other CD27L-sensitive bacteria.C lostridium sporogenes is environmental bacterium which has been associated with a range of food and silage spoilage problems (13,30,39,46). In cheese spoilage, it can contribute to gas formation and can also compound the damage caused by Clostridium tyrobutyricum, the major cause of cheese blowing (24,30).…”

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confidence: 99%

“…It is also physiologically comparable to a nontoxigenic form of proteolytic Clostridium botulinum (29) and as such is often used as a model organism for investigating treatments to reduce C. botulinum contamination. Investigations of methods of control have focused on reducing spore numbers or outgrowth by protocols such as centrifugation and brining (43), polyphosphates and heat treatment (7), acid-blanching and chelation (39), administration of lantibiotics (13,46), a combination of heat, acidification, and nisin (35), or pulsed pressurization (1). Nitrosyl complexes have also been shown to inhibit the growing cells (20).…”

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Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes

Mayer

Gasson

Narbad

2012

Appl Environ Microbiol

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ABSTRACTLytic bacteriophage ATCC 8074-B1 produces large plaques on its hostClostridium sporogenes. Sequencing of the 47,595-bp genome allowed the identification of 82 putative open reading frames, including those encoding proteins for head and tail morphogenesis and lysis. However, sequences commonly associated with lysogeny were absent. ORF 22 encodes an endolysin, CS74L, that shows homology toN-acetylmuramoyl-l-alanine amidases, and when expressed inEscherichia coli, the protein causes effective lysis ofC. sporogenescells when added externally. CS74L was also active onClostridium tyrobutyricumandClostridium acetobutylicum. The catalytic domain expressed alone (CS74L1–177) exhibited a similar activity and the same host range as the full-length endolysin. A chimeric endolysin consisting of the CS74L catalytic domain fused to the C-terminal domain of endolysin CD27L, derived fromClostridium difficilebacteriophage ΦCD27, was produced. This chimera (CSCD) lysedC. sporogenescells with an activity equivalent to that of the catalytic domain alone. In contrast, the CD27L C-terminal domain reduced the efficacy of the CS74L catalytic domain when tested againstC. tyrobutyricum. The addition of the CD27L C-terminal domain did not enable the lysin to targetC. difficileor other CD27L-sensitive bacteria.

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confidence: 99%

Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes

Mayer

Gasson

Narbad

2012

Appl Environ Microbiol

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“…La importancia de este microorganismo radica en los problemas de intoxicación oral por la toxina botulínica, la cual afecta el sistema nervioso, y produce náuseas, vómito y, en los casos más severos, parálisis muscular. Los estudios demuestran que bacteriocinas como la nisina tienen efecto antagónico sobre el microorganismo (27). Al respecto, Jurado-Gámez y colaboradores (28) encontraron inhibición de L. plantarum sobre Clostrium perfringen, lo cual muestra efectividad de la cepa contra el microorganismo patógeno.…”

Section: Discussionunclassified

Efecto bioconservante del sobrenadante de Lactobacillus plantarum y Lactobacillus lactis en lomo de cerdo (Longisimus dorsi)

Jurado-Gámez¹,

Jarrín-Jarrín²,

Bustamante-Melo³

2017

Rev. Med. Vet.

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Jurado-Gámez H, Jarrín-Jarrín V, Bustamante-Melo J. Efecto bioconservante del sobrenadante de Lactobacillus plantarum y Lactobacillus lactis en lomo de cerdo (Longisimus dorsi Palabras clave: bioconservante, bacteria ácido-láctica, inhibición, lomo de cerdo, sobrenadante. Bioconservant effect of the supernatant of Lactobacillus plantarum and Lactobacillus lactis on pork loin (Longisimus dorsi)Abstract Bioconservation extends the useful life of food and improves its quality. This paper aimed to determine the effect of the supernatant of Lactobacillus plantarum and Lactobacillus lactis on pork loin. The supernatant (500 μl) was used in 50 g of loin, and as controls, lactic acid (2%) and physiological saline (without additive). Each treatment was evaluated at 4 and 20 °C for 15 days. On days 0, 7 and 15, microbiological, physicalchemical and sensorial measurements were performed; the first two were evaluated with a 4×2 factorial block design. The block consisted of measurement days, and the factors were additive and temperature. Sensory evaluation was done by analysis of variance. There was no interaction between factors (p > 0.05). Total coliforms, sulfite-reducing clostridium, and pH showed no difference in the additive factor (p > 0.05), but fecal coliforms, acidity and water retention capacity did (p < 0.05). Refrigeration (4 °C) had

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“…Casings derived from animal intestines can be one possible route for transmission of C. perfringens spores and other sulphite-reducing anaerobic spores, since the brining process of intestines does not inactivate bacterial endospores. In one study, it was shown that nisin was partly reversibly bound to casings and can reduce the outgrowth of Clostridium sporogenes spores in the model used by approximately 1 log cycle (Wijnker et al 2011 ). This could open new possibilities to combat the entry of pathogens in the food chain.…”

Section: Semi-processed and Cooked Meatsmentioning

confidence: 99%

Biopreservation of Meats and Meat Products

Gálvez¹,

López²,

Pulido³

et al. 2014

Food Biopreservation

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Reduction of Clostridium sporogenes spore outgrowth in natural sausage casings using nisin

Cited by 35 publications

References 31 publications

Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes

Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes

Efecto bioconservante del sobrenadante de Lactobacillus plantarum y Lactobacillus lactis en lomo de cerdo (Longisimus dorsi)

Biopreservation of Meats and Meat Products

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