Reduction in trabecular meshwork stem cell content in donor eyes with primary open angle glaucoma

Abstract

We previously identified and characterized human trabecular meshwork stem cells (TMSCs) based on high expression of ABCG2/p75 positivity and high nucleus to cytoplasmic ratio. These TMSCs expressing high ABCG2 and p75 were located to the insert region of the human TM. Additionally, we demonstrated an age-related reduction in the TMSC content which was significantly associated with TM cell loss. In continuation, this study was aimed to determine the TMSC content in glaucomatous donor eyes wherein a drastic reduction in TM cellularity has already been reported. Anterior segments from known glaucomatous (n = 6) and age-matched normal (n = 8) donors were dissected into four quadrants. A minimum of three sections from each quadrant were used for histopathological analysis as well as immunostaining. Analysis of hematoxylin and eosin-stained sections from glaucomatous tissues revealed a decrease in total TM cellularity, thickening of trabecular beams, fusion of trabeculae, absence of patent Schlemm’s canal compared to age-matched controls. In addition, the TM thickness at various positions of the meshwork and the coronal as well as the meridional diameters of the Schlemm’s canal were observed to be significantly reduced in glaucomatous eyes. Further, sections from both the groups were immunostained for universal stem cell marker ABCG2 and neural crest derived stem cell marker p75. The images were acquired using Leica SP8 confocal microscope. Quantification of total TM cellularity based on nuclear counterstain (mean ± SD) using ImageJ identified 69.33 ± 12.77 cells/section in control eyes. In glaucomatous donors, the TM cellularity was found to be reduced significantly to 41.83 ± 9.0 (p = 0.0007). In addition, a reduction in the percentage of TMSCs (cells with high ABCG2 expression and p75 positivity) was evident in glaucomatous donors (0.14 ± 0.17%) compared to age-matched controls (4.73 ± 5.46%) (p = 0.064). Thus, the present study confirmed the significant decline in TM cellularity and a reducing trend in the TMSC content, though this reduction was non-significant in glaucomatous donor eyes. Further studies are essential to elucidate the role of TMSCs in the pathogenesis of primary open angle glaucoma.

Aging, Cellular Senescence, and Glaucoma

“…Aging results in a reduction in the number of cells in the outflow system of the normal eye, which is associated with some of the structural changes that occur as we age, such as trabecular thickening and fusion [ 9 , 10 ]. Compared with age-matched controls, patients with POAG have been found to lose a greater number of TM cells as they age [ 11 , 12 ]. Moreover, it has been suggested that the absence of TM cells, followed by their replacement with extracellular matrix (ECM) leads to increased resistance to fluid outflow in these patients, thus triggering an increase in IOP [ 13 ].…”

Aging, Cellular Senescence, and Glaucoma

“…Aging results in a reduction in the number of cells in the outflow system of the normal eye, which is associated with some of the structural changes that occur as we age, such as trabecular thickening and fusion [9,10]. Compared with agematched controls, patients with POAG have been found to lose a greater number of TM cells as they age [11,12]. Moreover, it has been suggested that the absence of TM cells, followed by their replacement with extracellular matrix (ECM) leads to increased resistance to fluid outflow in these patients, thus triggering an increase in IOP [13].…”