iPSCs-Based Therapy for Trabecular Meshwork

iPSC‐derived cells stimulate ABCG2+/NES+ endogenous trabecular meshwork cell proliferation and tissue regeneration

“…As described previously, 22 the cultured medium conditioned by human trabecular meshwork cells was pooled and sterilised by filtration through mixed cellulose ester membrane filters (0.2 μm pore size; Millipore, Danvers, MA) and applied to induced mouse iPSCs differentiation for 10 days. Differentiated iPSCs, named iPSC‐TM, were separated from undifferentiated cells using a magnetic bead‐based approach 22 and characterised as TM‐like cells since the expressions of TM biomarkers and dexamethasone‐induced myocilin expression and cross‐linked actin network formation 7,21–25 . iPSC‐TM generated following this protocol was used in this study.…”

“…Abnormalities in these structures have been linked to the pathogenesis of glaucoma and are considered the primary cause of irreversible blindness worldwide 2–6 . Recent evidence has increasingly revealled that pharmacological interventions and gene/stem cell‐based therapies targeting the TM and SC can efficiently enhance AH outflow and maintain the eye's intraocular pressure (IOP) homeostasis 7–11 …”

“…Moreover, our previous studies have revealled that endogenous cells can transit from a quiescent state to an active status immediately after interacting with induced pluripotent stem cell‐derived TM (iPSC‐TM) cells. This initial activation at the time of transplantation enables the TM to regenerate efficiently for an extended period, even though iPSC‐TM cells diminished after transplantation 7,21–25 . Thus, it is of particular interest to investigate the features of these highly responsive cells.…”

“… 2 , 3 , 4 , 5 , 6 Recent evidence has increasingly revealled that pharmacological interventions and gene/stem cell‐based therapies targeting the TM and SC can efficiently enhance AH outflow and maintain the eye's intraocular pressure (IOP) homeostasis. 7 , 8 , 9 , 10 , 11 …”

“…This initial activation at the time of transplantation enables the TM to regenerate efficiently for an extended period, even though iPSC‐TM cells diminished after transplantation. 7 , 21 , 22 , 23 , 24 , 25 Thus, it is of particular interest to investigate the features of these highly responsive cells. Nevertheless, our understanding of these cells remains limited.…”