Reduction in Matrix-Related Signal Suppression Effects in Electrospray Ionization Mass Spectrometry Using On-Line Two-Dimensional Liquid Chromatography

Pascoe, R.; Foley, Joe P.; Gusev, Arkady I.

doi:10.1021/ac0106694

Cited by 125 publications

(92 citation statements)

References 30 publications

(43 reference statements)

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“…Given the well-established susceptibility of LC-MS/MS techniques to matrix effects, 5,7,8 the impact of reconstituted water, sediments, and biota on the MS/MS ionization responses of the four PPCPs was carefully assessed in each extracted matrix as follows. Spiked samples were frozen at -20 8C and analyzed within 1 week.…”

Section: Matrix Effectsmentioning

confidence: 99%

“…However, a complication associated with LC-MS analysis is the potential for interactions with other matrix co-eluents, believed to arise from competition between the analyte ions and co-eluents for gas-phase emission in the electrospray ionization source. 5,6 As the number of co-eluents increases, their interactions with the target analytes interferes with adequate quantification, thereby hindering investigations. 5,7,8 Several approaches have been suggested for the resolution of matrix effects.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 As the number of co-eluents increases, their interactions with the target analytes interferes with adequate quantification, thereby hindering investigations. 5,7,8 Several approaches have been suggested for the resolution of matrix effects. Quantification via matrix-matched calibration has been suggested, but the availability of non-contaminated material is problematic when studying contaminated sites.…”

Section: Introductionmentioning

confidence: 99%

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Matrix effects on mass spectrometric determinations of four pharmaceuticals and personal care products in water, sediments, and biota

et al. 2009

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Simple analytical methods were developed for the extraction and determination of four pharmaceuticals and personal care products (PPCPs) from water, sediments, and biota. PPCPs were determined using tandem LC-MS in electrospray ionization mode, and interactions with matrix co-eluents were investigated. Extractions of water samples were performed using solid-phase extraction (SPE), sediments were extracted by pressurized liquid extraction (PLE), and biota was extracted by liquid extraction. The selected analytical methods yielded recoveries 61% in all matrixes. Matrix interactions were investigated throughout the linear range of quantification of each compound, revealing that dissolved salts had relatively minor effects on ionization (between 14% suppression to 12% enhancement), but that sediment and biota extracts caused significant matrix effects (ranging from 56% suppression to 25% enhancement). The direction and magnitude of matrix interactions reflected the physico-chemical properties of each analyte, particularly their pKa. Among the compounds analyzed in electrospray positive mode, carbamazepine was insensitive to matrix interactions, because it is a strong proton acceptor (pK a = 14.0). In contrast, atorvastatin (pK a = 4.5), a weaker proton acceptor, was particularly sensitive to matrix effects. For those compounds analyzed in negative-ion mode, sample alkalinity was found to be important. With a pK a of 10.4, 17a-ethinylestradiol generally exhibited matrix enhancement with increased sample alkalinity. However, the presence of acidic co-eluents contributed to matrix suppression. Lastly, TCS was particularly sensitive to matrix suppression, as its circumneutral pK a (7.9) caused even slight changes in sample pH to considerably impact ionization. We conclude that while different matrixes have clear impacts on ionization of these PPCPs, matrix effects can be quantified and overcome.Key words: pharmaceuticals and personal care products, matrix effects, LC-MS/MS, sediments, biota. Résumé :On a développé des méthodes analytiques simples pour l'extraction et la détermination de quatre produits pharmaceutiques et de soins personnels (PPSP) à partir de solutions aqueuses, de sédiments et du biote. On a déterminé les PPSP en faisant appel à la chromatographie liquide opérant en tandem avec spectrométrie de masse en mode d'ionisation par électronébulisation (CL-SM) et on a étudié les interactions avec les coéluants de la matrice. Les extractions à partir de solutions aqueuses ont été effectuées en utilisant l'extraction en phase solide (EPS) alors que les sédiments ont été soumis à une extraction avec un liquide pressurisé (ELP) et que le biote a été soumis à une extraction liquide. Dans l'ensemble des matrices, les méthodes analytiques choisies ont permis d'obtenir des taux de récupération d'au moins 61 %. On a étu-dié les interactions avec la matrice pour la plage entière de quantification de chaque composé et ces études ont révélé que les sels dissous n'ont que des effets mineurs sur l'ionisation (alla...

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Section: Matrix Effectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Matrix effects on mass spectrometric determinations of four pharmaceuticals and personal care products in water, sediments, and biota

et al. 2009

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“…This reduces the likelihood that an interfering peak, whether known or unknown, will be overlapped with the peak for a target analyte that is being quantified, which would lead to an overestimation of the concentration of the target analyte (Bailey et al, 2012). On the other hand, even when highly selective detection is used (e.g., high-resolution MS or tandem MS) the potential for additional separation and/or selectivity provided by a 2D method can improve accuracy by reducing matrix effects Groskreutz et al, 2012b;Pascoe et al, 2001). This is especially valuable in cases where isotope-labeled standards are either not available or prohibitively expensive, as the elimination of matrix effects enables achievement of good accuracy when using external standards for signal calibration .…”

Section: Data Analysis Software and Quantitation 275mentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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“…However, with the ever-increasing pace in drug discovery study, minimal sample pretreatment and automation are desired. Consequently, on-line 2-D chromatography with valve switching technology has seen more and more use lately (Mullett, 2007;Pascoe et al, 2001;Pol and Hyotylainen, 2008). Two-dimensional chromatography involves the transfer of a fraction or fractions of sample from a primary column to a secondary column for further separation.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%