Reduction by Phytate-reduced Soybean β-Conglycinin of Plasma Triglyceride Level of Young and Adult Rats

Aoyama, Toyohiko; Kohno, M.; Saito, Tsutomu; Fukui, Kensuke; Takamatsu, Kiyoharu; Yamamoto, Takashi; Hashimoto, Yukio; Hirotsuka, Motohiko; Kito, Makoto

doi:10.1271/bbb.65.1071

Cited by 73 publications

(70 citation statements)

References 18 publications

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“…3,7,8,[22][23][24] In our study the isoflavones were not determined in the b-conglycinin fraction; however, McVeigh et al 25 and Adams et al 26 showed in humans and monkeys that a soy protein diet low in isoflavones produces the same effect on cholesterol levels as a soy protein diet high in isoflavones. These products may be vastly different when comparing studies and=or distinct sources.…”

Section: B-conglycinin In Hypercholesterolemic Ratscontrasting

confidence: 54%

“…27 It can also be observed that in the vast majority of these studies the authors used these proteins as the only dietary protein source and employed various animal models, similar to those in experiments of nutritional evaluation. 5,8,10,11,27 In our study, however, the isolated bconglycinin was given in a single oral daily dose, after an extensive process of isolation (Fig. 1).…”

Section: B-conglycinin In Hypercholesterolemic Ratsmentioning

confidence: 81%

“…Fukui et al 7 and Aoyama et al 8 observed a significant reduction in TG in the plasma of rats, but the former did not observe any change in the level of TC, while the latter recorded a significant reduction. Moriyama et al 10 showed in normal and genetically obese mice that ingestion of b-conglycinin lowered the rate of fatty acid synthetic activity and increased that of b-oxidation enzymes in the liver; furthermore, the fecal excretion of TG was increased with respect to the controls.…”

Section: B-conglycinin In Hypercholesterolemic Ratsmentioning

confidence: 92%

“…These proteins constitute up to 90% of all the protein in the seed. The 7S fraction has been studied as the only source of dietary protein in various experimental designs, using various animals, such as rats, [7][8][9] normal and genetically obese mice, 10 and monkeys.…”

Section: Introduction Lmentioning

confidence: 99%

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Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Ferreira

Silva

Demonte

et al. 2011

Journal of Medicinal Food

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The aim of this study was to examine the comparative hypocholesterolemic effect of soybean 7S fraction in rats fed a high-cholesterol diet. Soybean 7S globulin (b-conglycinin) was administered orally once a day to rats, and the effects were measured after 28 days. Wistar rats were divided into four groups: standard diet (STD) (casein alone), hypercholesterolemic (HC) diet (STD plus 1 g=100 g cholesterol and 0.5 g=100 g cholic acid), HCþ7S 1 diet (HC diet plus 200 mg of 7S=kg of body weight=day), and HCþ7S 2 diet (HC diet plus 300 mg of 7S=kg of body weight=day). Food intake, weight gain, animals' growth, and feeding efficiency ratio were similar among the STD and three HC groups, indicating that these parameters were not affected by treatments. Animals that had received different doses of soybean 7S globulin had lower total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL)=high-density lipoprotein (HDL) ratio in serum and lower levels of hepatic TC and TG than those fed only the HC diet. The atherogenic indexes of HCþ7S 1 and HCþ7S 2 groups were 40% and 55% lower than that of the HC group, respectively. The results showed that the oral daily administration of bconglycinin in the diet to HC rats, at between 1.85% and 2.75% of total ingested protein, promotes the reduction of TC, LDLcholesterol, and TG and an increase in HDL-cholesterol in the plasma, besides a small but significant reduction in cholesterol and TG levels in the liver of the animals as well as a reduced atherogenic index.

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Section: B-conglycinin In Hypercholesterolemic Ratscontrasting

confidence: 54%

Section: B-conglycinin In Hypercholesterolemic Ratsmentioning

confidence: 81%

Section: B-conglycinin In Hypercholesterolemic Ratsmentioning

confidence: 92%

Section: Introduction Lmentioning

confidence: 99%

See 2 more Smart Citations

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Ferreira

Silva

Demonte

et al. 2011

Journal of Medicinal Food

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“…13) Furthermore, -conglycinin was found to have hypocholesterolemic activity in rats. 14) It will be interesting to find to what extent the hypocholesterolemic activity of -conglycinin in vivo is potentiated by this genetic modification. This will need to be tested after production of modifiedconglycinin in plants, since the yield in E.coli is too small for an in vivo study.…”

Section: Introduction Of Dpr An Enterostatin Fragment Peptide Into mentioning

confidence: 99%

Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α′ Subunit by Site-directed Mutagenesis

Takenaka

Doyama

Maruyama

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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High hydrostatic pressure treatment reduces the potential antigenicity of β‐conglycinin by changing the protein structure during in vitro digestion

2022

J Sci Food Agric

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BACKGROUND High hydrostatic pressure (HHP) treatment has been used to alleviate the allergenicity of soybeans, but there are little data about the potential antigenicity of β‐conglycinin after HHP treatment. RESULTS We examined the effects of HHP treatment on the antigenicity and structure of β‐conglycinin. When the pressure was 300 and 400 MPa, HHP treatment reduced the immunoglobulin (Ig)G binding capacity of β‐conglycinin, while its IgE binding capacity did not change significantly. After in vitro digestion, both the IgE and IgG binding of β‐conglycinin was obviously inhibited after HHP treatment at 400 MPa and 60 °C, although its binding capacity with linear epitope antibodies increased. Moreover, HHP treatment changed the secondary structure of β‐conglycinin, the content of α‐helix and random coils increased, while the β‐sheet and β‐turn decreased. After HHP treatment, the conformational structure was unfolded so that a large number of hydrophobic regions were exposed. CONCLUSION HHP treatment alleviated the potential antigenicity of β‐conglycinin by modifying its structure, which facilitated in vitro digestion and destroyed epitopes. This research provides a new insight into the mechanism of HHP treatment that affects the sensitization of soy protein allergens. © 2022 Society of Chemical Industry.

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Reduction by Phytate-reduced Soybean β-Conglycinin of Plasma Triglyceride Level of Young and Adult Rats

Cited by 73 publications

References 18 publications

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α′ Subunit by Site-directed Mutagenesis

High hydrostatic pressure treatment reduces the potential antigenicity of β‐conglycinin by changing the protein structure during in vitro digestion

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