Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms

Liang, Cong; Cao, Guangwen; Xue, Renyu; Pan, Zhijuan; Zheng, Xiaoya; Zhou, Wenlin; Gong, Chengliang

doi:10.1016/j.bbrc.2011.05.157

Cited by 13 publications

(16 citation statements)

References 17 publications

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“…The fibroin heavy chain silk of silkworms forms 2377 repeats of a GX dipeptide motif in which the X residues are Ala in 64%, Ser in 22%, Tyr in 10%, Val in 3%, and Thr in 1.3% of the repeats (Zhou et al, 2000). Interestingly, the biological effects of silkworm silk fibroin proteins reportedly include the enhancement of glucose (Hyun et al, 2004;Park et al, 2002;Jung et al, 2010;Cong et al, 2011;Do et al, 2012) and lipid metabolism (Jung et al, 2010), anti-human immunodeficiency virus activity (Gotoh et al, 2000), DNA damage protection (Park et al, 2002), and blood pressuredepressing activity (Igarashi et al, 2006).…”

Section: Introductionmentioning

confidence: 95%

Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Lee

Kim

et al. 2014

Journal of Asia-Pacific Entomology

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Section: Introductionmentioning

confidence: 95%

Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Lee

Kim

et al. 2014

Journal of Asia-Pacific Entomology

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“…The silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), is a powerful model for research and also an economically important insect to the silk industry (Park et al, 2010;Cong et al, 2011). B. mori synthesizes large amounts of silk proteins in its silk glands (Cong et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…B. mori synthesizes large amounts of silk proteins in its silk glands (Cong et al, 2011). Compared to the traditional value of B. mori as source of silk, its major role in the near future will be as a biomedical insect for producing biomaterials (Park et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Compared to the traditional value of B. mori as source of silk, its major role in the near future will be as a biomedical insect for producing biomaterials (Park et al, 2010). Therefore, the silkworm might be suited as a host for the mass production of recombinant proteins compared to mammalian and non-mammalian cultured cells (Cong et al, 2011). The expressions of target genes in transgenic insects have to regulate in a time-or tissue-specific manner the effective production of recombinant proteins.…”

Section: Introductionmentioning

confidence: 99%

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Hemocyte-specific Promoter for the Development of Transgenic Silkworm, Bombyx mori

Park¹,

Goo²,

Kim³

et al. 2012

International Journal of Industrial Entomology

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In previous studies we have shown that a sw17255 gene was expressed in hemocyte-specific tissues of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). It was verified that the sw17255 core promoter region contains elements that regulate the expression of this gene in hemocyte tissue; the selected promoter region spans nucleotides -1 to -2,112 upstream of the start codon. Each of the luciferase reporter gene expression vectors under the control of 4 different kinds of promoter candidates, (-2,112/-1), (-1,640/-1), (-1,169/-1) and (-579/-1), and the control reporter plasmid DNA, were introduced into B. mori larval coelom by direct injection using a syringe. The promoter candidate [E] (-579/-1) showed more than 1.67 fold transcriptional activity compared to control promoter activity. Higher productivity of an expressed gene in the transgenic silkworm by this promoter combination could be achieved in the near future. The foreign recombinant protein could be easily harvested from the blood of the transgenic silkworm.

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“…Since 1989 successful in vitro uptake of exogenous DNA by sperm cells of different animal species followed in most cases by post-fertilization transfer and maintenance of the transgene have been reported ( 9 ) . The SMGT procedure has been optimized in large mammals ( 8,20,21 ) and applied to several animal species from silkworm ( 22,23 ) to fi sh ( 24,25 ) , from chicken ( 26 ) to rabbit ( 27 ) , and from goat ( 28,29 ) to bovine ( 30 ) .…”

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confidence: 99%

Methods for Sperm-Mediated Gene Transfer

Lavitrano

Giovannoni

Cerrito

2012

Methods in Molecular Biology

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The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applications. Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.

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Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms

Cited by 13 publications

References 17 publications

Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Hemocyte-specific Promoter for the Development of Transgenic Silkworm, Bombyx mori

Methods for Sperm-Mediated Gene Transfer

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