Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Lee, Kwang-Sik; Kim, Bo Yeon; Kim, Doh Hoon; Jin, Byung Rae

doi:10.1016/j.aspen.2014.10.005

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Silk fibroin proteins from silkworms and spiders elicit very low levels of macrophage stimulation and are non-cytotoxicity, non-inflammatory, and exhibit low antigenicity when consumed by mammals [ 19 , 20 , 21 ]. They are well-known to have pharmacological activities, including anti-diabetic [ 10 , 11 , 12 , 13 , 14 , 22 ] and anti-obesity [ 23 , 24 , 25 , 26 ] properties. Silkworm and spider silk fibroin proteins enhance insulin secretion and reduce blood glucose levels [ 12 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…The supplementation of the transgenic rice seeds reduced the liver weights and blood glucose levels in the mice to levels similar to those observed in the control group. Glycine was previously reported to affect insulin secretion and reduce blood glucose levels [ 14 , 37 , 38 , 39 , 40 ]. Therefore, the high glycine content of the fibroin silk protein may be a key factor for this reduction of blood glucose levels in mice.…”

Section: Discussionmentioning

confidence: 99%

“…Silk fibroin proteins have also been reported to have therapeutic effects when consumed by mammals, including the enhancement of glucose [ 9 , 10 , 11 , 12 , 13 , 14 ] and lipid [ 12 ] metabolism, anti-HIV activity [ 15 ], DNA damage protection [ 11 ], and the reduction of blood pressure [ 16 ]. Diabetes and hypercholesterolemia are key causes of death in the global adult population.…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported the cloning and expression of partial cDNAs encoding the C-terminus of the spider ( Araneus ventricosus ) major ampullate silk protein (AvMaSp), which was found to enhance insulin secretion and reduce blood glucose levels when consumed by diabetic mice [ 7 , 14 ]. We found that the recombinant AvMaSp spider silk fibroin protein exhibits a non-cytotoxic and non-inflammatory response [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice

Yang

Lee

Hur

et al. 2020

Plants

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Silk fibroin proteins are biomaterials with diverse applications. These spider and silkworm proteins have specific biological effects when consumed by mammals; in addition to reducing blood pressure and blood glucose and cholesterol levels, they have anti-human immunodeficiency virus activity. In the present study, rice (Oryza sativa) was engineered to produce the C-terminus of the major ampullate spidroin protein from the spider Araneus ventricosus under the control of a Prolamin promoter. Homozygous transgenic rice lines were identified, and the therapeutic effect of this spider silk fibroin protein on the lipid and glucose metabolism was analyzed in a mouse model. Feeding fat-fed mice, the transgenic rice seeds for four weeks reduced serum concentrations of triglycerides, total cholesterol, low-density lipoprotein cholesterol, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase, and lowered blood glucose levels. This is the first study to investigate the effects of consumption of rice seeds heterologously expressing spider silk fibroin protein in a mammalian model. Our findings suggest that functional foods containing spider silk fibroin protein might be useful as potential pharmaceutical materials for preventing and treating diabetes, hyperlipidemia, and hypercholesterolemia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice

Yang

Lee

Hur

et al. 2020

Plants

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“…The biological effects of SPF include the enhancement of glucose (Hyun et al, 2004; Do et al, 2012) and lipid metabolism (Jung et al, 2010), anti-viral activity (Gotoh et al, 2000), DNA damage protection (Park et al, 2002), and blood pressure-depressing activity (Igarashi et al, 2006). Recombinant spider SPF exhibits non-cytotoxic and non-inflammatory effects in NIH 3T3 cells (Lee et al, 2016) and also reduces blood glucose levels in diabetic mice (Lee et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Dietary Exposure to Transgenic Rice Expressing the Spider Silk Protein Fibroin Reduces Blood Glucose Levels in Diabetic Mice: The Potential Role of Insulin Receptor Substrate-1 Phosphorylation in Adipocytes

Park¹,

Jeong²,

Park³

et al. 2019

Dev. Reprod.

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Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance (IR). T2DM is correlated with obesity and most T2DM medications have been developed for enhancing insulin sensitivity. Silk protein fibroin (SPF) from spiders has been suggested as an attractive biomaterial for medical purposes. We generated transgenic rice (TR) expressing SPF and fed it to diabetic BKS.Cg-m+/+Leprdb mice to monitor the changes in blood glucose levels and adipose tissue proteins associated with energy metabolism and insulin signaling. In the present study, the adipocyte size in abdominal fat in TR-SPF-fed mice was remarkably smaller than that of the control. Whereas the adenosine monophosphate-activated protein kinase (AMPK)-activated protein kinase and insulin receptor substrate 1 (IRS1) protein levels were increased in abdominal adipose tissues after TR-SPF feeding, levels of six-transmembrane protein of prostate 2 (STAMP2) proteins decreased. Phosphorylation of AMPK at threonine 172 and IRS1 at serine 307 and tyrosine 632 were both increased in adipose tissues from TR-SPF-fed mice. Increased expression and phosphorylation of IRS1 at both serine 307 and tyrosine 632 in adipose tissues indicated that adipocytes obtained from abdominal fat in TR-SPF-fed mice were more susceptible to insulin signaling than that of the control. STAMP2 protein levels decreased in adipose tissues from TR-SPF-fed mice, indicating that STAMP2 proteins were reducing adipocytes that were undergoing lipolysis. Taken together, this study showed that TR-SPF was effective in reducing blood glucose levels in diabetic mice and that concurrent lipolysis in abdominal adipocytes was associated with alterations of AMPK, IRS1, and STAMP2. Increased IRS1 expression and its phosphorylation by TR-SFP were considered to be particularly important in the induction of lipolysis in adipocytes, as well as in reducing blood glucose levels in this animal model.

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Spider silk fibers

Shehata

Hassounah

Sobolciak

et al. 2019

Materials for Biomedical Engineering

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Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

Cited by 8 publications

References 21 publications

Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice

Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice

Dietary Exposure to Transgenic Rice Expressing the Spider Silk Protein Fibroin Reduces Blood Glucose Levels in Diabetic Mice: The Potential Role of Insulin Receptor Substrate-1 Phosphorylation in Adipocytes

Spider silk fibers

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