Reduced-stature <i>Rosa</i> species through in vitro mutagenesis

BaigM., M. Q.; Hafizi, Akram; AbbasiN., A.; YaseenM.,; AkramZ.,; DonnellyD., J.

doi:10.4141/cjps2011-199

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Dwarfism was found by Kukimura et al (1976) in Cryptomeria and in Jasminum . Shorter internodes resulting in lower plant height were also observed in Populus and Rosa (Baig et al, 2012;Nishiguchi et al, 2012). Other variations in Rosa were restoration of fertility and changes in color (orange, pink, etc.…”

Section: Woody Trees and Shrubsmentioning

confidence: 92%

Mutation Breeding in Ornamentals

Melsen¹,

Wouw²,

Contreras³

2021

horts

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The promising possibilities of mutation breeding in ornamental plants have led to a great interest in effective mutagenic treatment protocols for various species. This review discusses mutagenic treatments of a large number of ornamental genera, the advantages and disadvantages of various techniques, and the possibilities of improving the associated protocols. A number of nontargeted mutagenesis methods are available, ranging from chemical treatment with alkylating agents to irradiation with X-rays, gamma rays, and neutron or heavy ion beams at various doses. These are all relatively inexpensive and have been proven to be effective mutagens in a large number of diverse species. Genetic engineering, however, remains mostly impractical for many ornamental breeding operations because of the high cost and lack of knowledge necessary to successfully transform and regenerate ornamental crops. Of the available nontargeted mutagens, irradiation with gamma rays is still the most popular. It provides high consistency compared with chemical mutagens, albeit at a seemingly lower mutagenic efficiency. Changes in the radiation dose rate may increase the efficiency, although chronic irradiation over a longer period causes fewer deleterious mutations than the commonly used acute irradiation protocols. Heavy ion beam irradiation may also provide highly consistent mutation induction at higher efficiencies because of the high particle energy associated with these treatments. There are also opportunities to improve chemical mutagenesis. Although the required knowledge of specific gene functions in many ornamentals is still lacking, combination mutagenesis with ethyl methanesulfontate with genetic screening in a process known as TILLING (Targeting Induced Local Lesions IN Genomes) may lead to a powerful mutation breeding tool in the future. Mutation breeding is still very useful, and many opportunities are available to improve the existing methods.

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Section: Woody Trees and Shrubsmentioning

confidence: 92%

Mutation Breeding in Ornamentals

Melsen¹,

Wouw²,

Contreras³

2021

horts

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“…For the practical application of applied mutagenesis, it is also crucial to determine the radiosensitivity of various cultivars of the same crop. Gamma radiation mutation frequency varies depending on cultivar and dose (Baig et al 2012). Certain cultivars are slightly more sensitive to mutagens than others, while others are resistant to them altogether.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effect of Different Doses of Gamma Radiation to Induce Variation in in vitro Raised Plants of Chrysanthemum

Chowdhury,

Hoque,

Sarker

2023

Plant Tissue Cult. & Biotech.

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Variation induction using gamma radiation in in vitro raised chrysanthemum is one of the most useful methods in floriculture. Different doses of gamma radiation have a negative effect on the in vitro shoot regeneration rate. Irradiation of in vitro micro shoots with highest dose 25Gy produced only 3.90% and 9.93% of BRAI Chry-1 and BARI Chry-2 micro shoots. But the lowest dose 5Gy could regenerate the highest percentage of shoots for both varieties. The LD50 obtained from the survival percentage of the irradiated in vitro raised shoots was found at 9.25 Gy for BARI Chry-1 and 11.19 for BARI Chry-2 variety. Successful mutation can be found around the LD50 doses of a specific genotype. During hardening of the M1V5 regenerants of BARI Chry-1 and BARI Chry-2 raised from 15Gy irradiated micro shoots showed highest variation regarding leaf structure, size of the internode and plant height. M1V5 regenerants developed from 10Gy of both varieties produced 20 to 50 percent variations. In future gamma radiation dose between 10-15Gy would be more effective to get variation in this flower. Plant Tissue Cult. & Biotech. 33(2): 155-165, 2023 (December)

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“…Inducing mutation in combination with in-vitro culture methods is now being considered as an effective method for plant improvement in several vegetative propagated crops such as flowers and ornamental plants. Many pieces of research on invitro mutagenesis rose by gamma-ray irradiation had been published (Smilansky et al, 1986;Datta, 1997;Arnold et al, 1998;Mohan Jain, 2006;Chakrabarty and Datta, 2010;Kahrizi et al, 2011;Baig et al, 2012;Aamir et al, 2016;Bala and Singh, 2016). Many new rose varieties were created in the world and developed for commercial production by the use of in-vitro mutation-assisted breeding techniques, typically three mutant rose varieties: Rosmarin, Yulikara, and Rosanda (Jain, 2006).…”

Section: Introductionmentioning

confidence: 99%

Creation of Variation through In-vitro Mutagenesis using Gamma radiation in Rose (Rosa hybrida L.) Variety ‘Lửa’

Le¹,

Nguyen²,

Lê³

2022

CTUJS

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Rosa hybrida L. var. Lửa has been one of the most important roses for a long time and is most often used for ornamental purposes in Sadec city, Dong Thap province. The study aimed to induce variation through in-vitro gamma-ray irradiation in the rose variety ‘Lửa’ for further improvement in rose breeding. In-vitro single node cuttings (25 in-vitro cuttings per treatment with four replications) were irradiated with different doses of γ-rays (0, 5, 10, 15, 20, 25, or 30 Gy) using a 60Co source (India) at Da Lat Nuclear Research Institute. The γ-irradiated explants were then cultured aseptically on Murashige and Skoog’s basal medium supplemented with 1.0 mg/L BAP to induce multiple shoots, shoot proliferation, and acclimatization at Division of Vegetables, Flowers and Landscaping of Southern Horticultural Research Institute (SOFRI). The LD50 dose was determined at 20 - 25 Gy treatments and these doses affected the multiplication rate, growth in-vitro, and ex-vitro survival rate. Three types of mutants with altered or novel flower color in comparison to the original flower color were isolated, such as Type 1 with orange-pink (Red 52C), Type 2 with pink (Red 54B), and Type 3 with Red 45AB with Red 54B bicolor.

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Reduced-stature Rosa species through in vitro mutagenesis

Cited by 5 publications

References 12 publications

Mutation Breeding in Ornamentals

Mutation Breeding in Ornamentals

Evaluation of the Effect of Different Doses of Gamma Radiation to Induce Variation in in vitro Raised Plants of Chrysanthemum

Creation of Variation through In-vitro Mutagenesis using Gamma radiation in Rose (Rosa hybrida L.) Variety ‘Lửa’

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