Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis

Amer, Mariano; Elverdín, Juan C.; Fernández‐Solari, Javier; Medina, Vanina Araceli; Chiarenza, A.P.; Vacas, María I.

doi:10.1016/j.archoralbio.2010.11.004

Cited by 20 publications

(28 citation statements)

References 39 publications

(64 reference statements)

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“…In the present study we demonstrated that 15 days of EP induced in rats produced a considerable decrease in SMG salivary secretion, indicating that periodontitis severely altered the SMG functionality. In agreement with these results, we have recently reported that 7 days of EP caused a drastic reduction in the capacity of SMG to secrete saliva in response to cholinergic stimulation [15]. The inhibitory effect on salivation induced by EP was partially prevented by treatment with a low concentration of histamine administered subcutaneously.…”

Section: Discussionsupporting

confidence: 76%

“…** P \ 0.01, *** P \ 0.001 vs. control. ## P \ 0.01, ### P \ 0.001 vs. experimental periodontitis data in which EP produced a two-fold increase in alveolar bone loss [15,53,55]. Interestingly, this detrimental effect was diminished by histamine treatment.…”

Section: Discussionmentioning

confidence: 83%

“…Measurement of prostaglandin E (PGE) content was performed as previously described [15]. Briefly, gingiva were homogenized in 1 ml ice-cold ethanol (100%), centrifuged at 10,000g for 15 min at 4°C, and the supernatants were collected and evaporated in a Speed-Vac.…”

Section: Determination Of Pge Content By Specific Radioimmunoassaymentioning

confidence: 99%

“…Recently, we have reported that experimental periodontitis (EP) reduced methacholine-induced SMG salivary secretion while increasing prostaglandin E content [15]. Furthermore, treatment with lipopolysaccharide, a microbial component of the plaque biofilm, reduced SMG salivation [16,17].…”

Section: Introductionmentioning

confidence: 99%

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Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Prestifilippo

Carabajal

Croci³

et al. 2012

Inflamm. Res.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 83%

Section: Determination Of Pge Content By Specific Radioimmunoassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Prestifilippo

Carabajal

Croci³

et al. 2012

Inflamm. Res.

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“…Research in rats showed that hypoxia (HX) plays an important role in the pathogenesis of periodontitis (Xiao et al, 2012). In an experimental periodontitis model, a close association between damage to the submandibular gland (SMG) and changes in inflammatory mediators such as inducible nitric oxide synthase (iNOS) and prostaglandin E 2 (PGE 2 ) has been found (Amer et al, 2011). Nitric oxide (NO) produced by iNOS during inflammatory events plays an important role in the host immune system, inducing vasodilatation and cell recruitment during periodontitis (Ossola et al, 2012).…”

mentioning

confidence: 99%

Lead Intoxication Under Environmental Hypoxia Impairs Oral Health

Terrizzi

Fernández‐Solari

Lee

et al. 2014

Journal of Toxicology and Environmental Health, Part A

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We have reported that chronic lead intoxication under hypoxic environment induces alveolar bone loss that can lead to periodontal damage with the subsequent loss of teeth. The aim of the present study was to assess the modification of oral inflammatory parameters involved in the pathogenesis of periodontitis in the same experimental model. In gingival tissue, hypoxia increased inducible nitric oxid synthase (iNOS) activity (p < .01) and meanwhile lead decreased prostaglandin E2 (PGE2) content (p < .05). In submandibular gland (SMG), iNOS activity was enhanced by lead and PGE2 content was increased by both lead and hypoxia (p < .01) and even more by combined treatments (p < .001). In the SMG, hypoxia stimulated angiogenesis (p < .01) with blood extravasation. Adrenal glands were 22% bigger in those animals exposed to lead under hypoxic conditions. Results suggest a wide participation of inflammatory markers that mediate alveolar bone loss induced by these environmental conditions. The lack of information regarding oral health in lead-contaminated populations that coexist with hypoxia induced us to evaluate the alteration of inflammatory parameters in rat oral tissues to elucidate the link between periodontal damage and these environmental conditions.

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A new target to ameliorate the damage of periodontal disease: The role of transient receptor potential vanilloid type‐1 in contrast to that of specific cannabinoid receptors in rats

Ossola

Balcarcel

Astrauskas

et al. 2019

Journal of Periodontology

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Background Transient receptor potential vanilloid type‐1 (TRPV1) is expressed in oral tissues cells and its activity can be regulated by inflammation products and anandamide. The aim of the present study was to evaluate the effects of blocking TRPV1 or specific cannabinoid receptors 1 (CB1r) and 2 (CB2r) on periodontal status of rats subjected to experimental periodontitis (EP). Methods Male rats were distributed in groups 1) control, 2) lipopolysaccharide‐induced EP (LPS), and 3) LPS plus capsazepine (Capz, TRPV1 antagonist) application (LPS+Capz). EP was induced by injections of LPS (1 mg/mL) around first molars and treatment was performed with Capz (2 µg/mL) applied locally during 6 weeks. Additional experiment was performed by applying CB1r and CB2r antagonists (AM251 and AM630) to rats with EP. Results Capz prevented alveolar bone loss (ABL) on the external crests and in the interradicular bone of the first molars (periodontal space height: LPS, 270.7 ± 33.5µm versus LPS+Capz, 216.4 ± 19.9 µm; P <0.01). Inflammatory mediators, like tumor necrosis factor‐alpha and prostaglandin E2, increased by LPS‐induced EP, were diminished in gingival tissue of rats treated with Capz. In contrast, application of AM251 and AM630 exacerbated ABL and gingival inflammatory mediators, increased by LPS, altering also biomechanical properties. Conclusions TRPV1 blockade attenuates periodontal impairment in EP rats, since it reduces local inflammation, unlike CB1r and CB2r blockade. This work lays the foundation for developing therapeutics in humans based on the pharmacological manipulation of these receptors to treat periodontal disease.

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Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis

Cited by 20 publications

References 39 publications

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Lead Intoxication Under Environmental Hypoxia Impairs Oral Health

A new target to ameliorate the damage of periodontal disease: The role of transient receptor potential vanilloid type‐1 in contrast to that of specific cannabinoid receptors in rats

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