Reduced lysis at high concentrations of lysozyme

Wilcox, F. H.; Daniel, Louise J.

doi:10.1016/0003-9861(54)90128-9

Cited by 24 publications

(9 citation statements)

References 6 publications

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“…In this study we tried to find out whether lysozyme activity can be determined by a turbidimetric method for clinical use. As was previously stated by WiLcox & DANIEL (1954), PARRY, GHANDAN & SHAHANI (1965) and HARRI-SON & SwiNGLER (1971), autoinhibition occurs in high lysozyme concentrations. This inhibition can be reduced by increasing the sodium ion concentration and by making the necessary dilutions to bring the samples within the range of the method.…”

Section: T-valuessupporting

confidence: 70%

“…Processing of saliva -It has been shown that high lysozyme concentrations, more than 20 [ig/ ml (hen's egg white), may inhibit lysozyme activity (HARRISON & SWINGLER 1971); this inhibition can be minimized by measuring the initial reaction time and by adding saline and working with dilutions of samples (WILCOX & DANIEL 1954). Mixed saliva samples here were diluted yk with saline (0.9%).…”

Section: Methodsmentioning

confidence: 99%

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Lysozyme activity and flow rate of mixed saliva in children, adolescents and adults

Raeste

Tuompo

1976

European J Oral Sciences

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– Reports of lysozyme activity in mixed saliva differ greatly from lysozyme determination in pure form. In this study lysozyme activity of mixed saliva was determined by turbidimetric method in healthy children, adolescents, and adults, 111 subjects altogether. The salivary flow rate was estimated. The lysozyme activity of mixed saliva in 24‐ children was 129.4 ± 42.4, in 21 adolescents 160.9 ± 58.2, and in 66 adults 165.7 ± 36.7 (|ig/ml hen's egg white lysozyme). The flow rate of mixed saliva was similar in different groups. No statistically significant linear dependence between saliva lysozyme and flow rate was found. The lysozyme activities obtained correspond with the results of saliva lysozyme in pure form.

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Section: T-valuessupporting

confidence: 70%

Section: Methodsmentioning

confidence: 99%

Lysozyme activity and flow rate of mixed saliva in children, adolescents and adults

Raeste

Tuompo

1976

European J Oral Sciences

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“…Thus, the slow dialysis component might correspond to the monomeric form of lysozyme, while the fast dialysis components may be associated products. The apparently contradictory dialysis behavior of monomers (Cs), which have smaller Stokes radii than the associated species, can be explained by the adsorption of monomers on the cellulose membrane surface, as has been reported for several basic peptides and proteins (8,17,(19)(20)(21)(22)(23).…”

Section: Resultsmentioning

confidence: 79%

A biophysical model of lysozyme self-association

Hampe¹,

Tondo²,

A³

1982

Biophysical Journal

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The concentration dependence of the self-association of hen egg-white lysozyme was studied spectrophotometrically at pH 6, 25 degrees C, and low ionic strength within a concentration range of 2.5-50 micrograms/ml. Of several possible mathematical models, an ideal or nearly ideal two-stage model representing an equilibrium between monomers and dimers and between dimers and trimers best describes the data. The dimerization and trimerization constants were found to be 2.5 x 10(-2) and 38 x 10(-2). Dialysis experiments confirmed that the mechanism involves three associating species. A "head-to-tail" contact between the associating sites was inferred from dialysis studies of the effect of indole and imidazole derivatives on lysozyme self-association.

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“…Consequently, even in undersaturated solutions, increasing the concentration of lysozyme should increase aggregate formation leading to decreases in its enzymatic activity. Experimental measurements have shown that the activity does indeed decrease with the concentration (Wilcox & Daniel, 1954;Hampe et al, 1982). Other studies of the aggregation process have further indicated that the dimers formed are not the 'tail-to-tail' or 'sideto-side' dimers (i.e.…”

Section: Formation Of the Cr3'stallizing Unitsmentioning

confidence: 96%

Growth Mechanism and Morphology of Tetragonal Lysozyme Crystals

Nadarajah¹,

Pusey²

1996

Acta Cryst D

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The tetragonal form of hen egg-white lysozyme is the most investigated protein crystal for growth studies, but the relationship between its surface morphology and internal structure is still not well understood. One method of determining this relationship for inorganic crystals is by employing the periodic bond chain (PBC) theory of Hartman & Perdok [Hartman & Perdok (1955).Acta Co'st. 8,[49][50][51][52][521][522][523][524][525][526][527][528][529]. However, complexities resulting from the packing arrangements and the number of intermolecular bonds in protein crystals have resulted in the use of only simplified versions of this theory so far. In this study a more complete PBC analysis of tetragonal lysozyme crystals was carried out, coupled with an approach incorporating the molecular orientations of the crystal structure. The analysis revealed the existence of a helical tetramer building block of the entire crystal structure, centered around the 43 crystallographic axes, resulting in doublelayered slices and PBC's throughout. The analysis also indicated that the crystallizing units for the faces are at least as large as this tetramer, with the experimental evidence suggesting that it is a tetramer unit for the { 101 } faces and an octamer unit for the {110} faces. The { 110} faces were shown to be molecularly smooth F faces, while the { 101 } to be essentially rough S faces. The predicted morphology and growth mechanisms were found to explain numerous experimental observations from electron and atomic force microscopy, etching studies, lysozyme aggregation studies and measurements of growth kinetics.

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Reduced lysis at high concentrations of lysozyme

Cited by 24 publications

References 6 publications

Lysozyme activity and flow rate of mixed saliva in children, adolescents and adults

Lysozyme activity and flow rate of mixed saliva in children, adolescents and adults

A biophysical model of lysozyme self-association

Growth Mechanism and Morphology of Tetragonal Lysozyme Crystals

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