Reduced levels of both circulating CD4+CD25+CD127low/neg and CD4+CD8neg invariant natural killer regulatory T cells in stable heart transplant recipients

Abstract

SummaryA cross-regulation between two regulatory T cell (Treg) subsets [CD4 + CD25 + and invariant natural killer (NK) T -iNK T] has been described to be important for allograft tolerance induction. However, few studies have evaluated these cellular subsets in stable recipients as correlates of favourable clinical outcome after heart transplantation. Treg and iNK T cell levels were assayed by flow cytometry in peripheral blood samples from 44 heart transplant recipients at a 2-year interval in 38 patients, and related to clinical outcome. Multiparameter flow cytometry used CD4/CD25/CD127 labelling to best identify Treg, and a standard CD3/CD4/CD8/Va24/Vb11 labelling strategy to appreciate the proportions of iNK T cells. Both subtypes of potentially tolerogenic cells were found to be decreased in stable heart transplant recipients, with similar or further decreased levels after 2 years. Interestingly, the patient who presented with several rejection-suggesting incidents over this period displayed a greater than twofold increase of both cell subsets. These results suggest that CD4 + CD25 + CD127 low/neg Treg and iNK T cells could be involved in the local control of organ rejection, by modulating immune responses in situ, in clinically stable patients. The measurement of these cell subsets in peripheral blood could be useful for non-invasive monitoring of heart transplant recipients, especially in the growing context of tolerance-induction trials.

Comparison of postoperative lymphocytes and interleukins between laparoscopy-assisted and open radical gastrectomy for early gastric cancer

“…Notably, a low CD4þ/CD8þ ratio is also generally considered as a predictor for the severity of disease or a poor prognosis. 16,17 Accordingly, our results indicated that less trauma and effects on immune function resulting from laparoscopic surgery might be good for recovery postoperatively and have potential benefits on long-term survival.…”

Circulating lymphocyte subsets in different clinical situations after renal transplantation

“…, 26,27 showing reduced numbers of circulating CD4 + CD25 high Foxp3 + regulatory T cells in chronically rejecting patients compared with patients with stable graft function. Reduced percentages of circulating CD25 + CD127 low CD4 + T cells were also found in stable heart transplant recipients at 2 years after transplantation 28 . This might reflect a sequestration of these cells in the allograft.…”

“…Reduced percentages of circulating CD25 + CD127 low CD4 + T cells were also found in stable heart transplant recipients at 2 years after transplantation. 28 This might reflect a sequestration of these cells in the allograft. Indeed, in a murine kidney allograft model, a higher and sustained level of Foxp3 + regulatory T cells has been demonstrated in tolerated compared with rejecting allografts.…”

Effects of laparoscopic radical gastrectomy and the influence on immune function and inflammatory factors

“…CD4 + /CD8 can reflect the immune balance of an organism, if the ratio is low, it means that body immune function is decreasing and may increase tumor proliferation. Of note, a low ratio is commonly regarded as the index for severe disease or a poor prognosis (21)(22)(23). In the present study, CD3 + , CD4…”