Reduced graphene oxide-embedded nerve conduits loaded with bone marrow mesenchymal stem cell-derived extracellular vesicles promote peripheral nerve regeneration

Zhang, Wei; Fang, Xingxing; Li, Qi-Cheng; Pi, Wei; Han, Na

doi:10.4103/1673-5374.343889

Cited by 23 publications

(14 citation statements)

References 72 publications

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“…GelMA hydrogels can be loaded with active factors or drugs to improve the immune microenvironment and stimulate nerve repair and regeneration [141,[146][147][148][149]. Zhang et al [150] prepared an rGO-GelMA-PCL nerve conduit system by combining reduced graphene oxide (rGO) with GelMA and polycaprolactone (PCL), which was loaded with extracellular vesicles of bone marrow mesenchymal stem cells (BMSCs) and found to significantly repair peripheral nerves and promote vascular regeneration at the site of injury.…”

Section: Gelma For Peripheral Nerve Injury (Pni)mentioning

confidence: 99%

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Lv¹,

Li²,

Hu³

et al. 2023

Theranostics

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Increasing data reveals that gelatin that has been methacrylated is involved in a variety of physiologic processes that are important for therapeutic interventions. Gelatin methacryloyl (GelMA) hydrogel is a highly attractive hydrogels-based bioink because of its good biocompatibility, low cost, and photo-cross-linking structure that is useful for cell survivability and cell monitoring. Methacrylated gelatin (GelMA) has established itself as a typical hydrogel composition with extensive biomedical applications. Recent advances in GelMA have focused on integrating them with bioactive and functional nanomaterials, with the goal of improving GelMA's physical, chemical, and biological properties. GelMA's ability to modify characteristics due to the synthesis technique also makes it a good choice for soft and hard tissues. GelMA has been established to become an independent or supplementary technology for musculoskeletal problems. Here, we systematically review mechanism-of-action, therapeutic uses, and challenges and future direction of GelMA in musculoskeletal disorders. We give an overview of GelMA nanocomposite for different applications in musculoskeletal disorders, such as osteoarthritis, intervertebral disc degeneration, bone regeneration, tendon disorders and so on.

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Section: Gelma For Peripheral Nerve Injury (Pni)mentioning

confidence: 99%

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Lv¹,

Li²,

Hu³

et al. 2023

Theranostics

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“…From the tissue engineering perspective, the neural conduits require both, high conductivity and biocompatibility. 55 Moreover, conductive biomaterials can be used to promote the biological function of cells, in particular differentiation of stem cells. 56 In addition, conduits have to mimic the complex topographical structure of the nerve fibers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The sinusoidal current flow was caused by the nanometer-sized electron wells distributed at nanometer distances (gaps between 2D MXene sheets). From the tissue engineering perspective, the neural conduits require both, high conductivity and biocompatibility . Moreover, conductive biomaterials can be used to promote the biological function of cells, in particular differentiation of stem cells .…”

Section: Resultsmentioning

confidence: 99%

Polycaprolactone–MXene Nanofibrous Scaffolds for Tissue Engineering

Diedkova

Pogrebnjak

Kyrylenko

et al. 2023

ACS Appl. Mater. Interfaces

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New conductive materials for tissue engineering are needed for the development of regenerative strategies for nervous, muscular, and heart tissues. Polycaprolactone (PCL) is used to obtain biocompatible and biodegradable nanofiber scaffolds by electrospinning. MXenes, a large class of biocompatible 2D nanomaterials, can make polymer scaffolds conductive and hydrophilic. However, an understanding of how their physical properties affect potential biomedical applications is still lacking. We immobilized Ti 3 C 2 T x MXene in several layers on the electrospun PCL membranes and used positron annihilation analysis combined with other techniques to elucidate the defect structure and porosity of nanofiber scaffolds. The polymer base was characterized by the presence of nanopores. The MXene surface layers had abundant vacancies at temperatures of 305− 355 K, and a voltage resonance at 8 × 10 4 Hz with the relaxation time of 6.5 × 10 6 s was found in the 20−355 K temperature interval. The appearance of a long-lived component of the positron lifetime was observed, which was dependent on the annealing temperature. The study of conductivity of the composite scaffolds in a wide temperature range, including its inductive and capacity components, showed the possibility of the use of MXene-coated PCL membranes as conductive biomaterials. The electronic structure of MXene and the defects formed in its layers were correlated with the biological properties of the scaffolds in vitro and in bacterial adhesion tests. Double and triple MXene coatings formed an appropriate environment for cell attachment and proliferation with mild antibacterial effects. A combination of structural, chemical, electrical, and biological properties of the PCL−MXene composite demonstrated its advantage over the existing conductive scaffolds for tissue engineering.

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“…110,111 All these graphene forms have been extensively used to realize scaffolds, showing improved nerve regeneration and neurite sprouting and outgrowth. 43,[112][113][114][115][116] In contrast, the effect of monolayer CVD graphene on MSCs is still little explored. CVD graphene has been demonstrated to promote the neurogenesis of hMSCs as well as the neurite outgrowths by enhancing MSC-substrate and cell-cell interactions.…”

Section: Discussionmentioning

confidence: 99%

Interaction of graphene and WS₂ with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Convertino,

Nencioni,

Russo

et al. 2024

Nanoscale

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Reduced graphene oxide-embedded nerve conduits loaded with bone marrow mesenchymal stem cell-derived extracellular vesicles promote peripheral nerve regeneration

Cited by 23 publications

References 72 publications

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Polycaprolactone–MXene Nanofibrous Scaffolds for Tissue Engineering

Interaction of graphene and WS₂ with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

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Reduced graphene oxide-embedded nerve conduits loaded with bone marrow mesenchymal stem cell-derived extracellular vesicles promote peripheral nerve regeneration

Cited by 23 publications

References 72 publications

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Polycaprolactone–MXene Nanofibrous Scaffolds for Tissue Engineering

Interaction of graphene and WS2 with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

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Interaction of graphene and WS₂ with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration